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Effects of inhibition of glycation and oxidative stress on the development of diabetic nephropathy in rats.

Agardh, Carl David ; Stenram, Unne LU ; Torffvit, Ole LU and Agardh, Elisabet LU (2002) In Journal of Diabetes and its Complications 16(6). p.395-400
Abstract
We investigated whether aminoguanidine (AG), an inhibitor of advanced glycated end product formation, or probucol (PB), a free radical scavenger, could influence signs of glomerular and distal tubular function and morphological changes in kidneys of male Wistar rats after 6 months of streptozocin (STZ)-induced diabetes. Diabetic rats had a higher kidney weight/body weight ratio (P<.001), but neither AG nor PB influenced the increased ratio. Diabetes caused an increased urinary albumin excretion (P<.05), which was normalized by AG, but further exaggerated by PB (P<.001). Diabetes also caused an increase in the urinary excretion of Tamm–Horsfall protein (P<.001). Both AG and PB attenuated this increase (P<.05 for both). A few... (More)
We investigated whether aminoguanidine (AG), an inhibitor of advanced glycated end product formation, or probucol (PB), a free radical scavenger, could influence signs of glomerular and distal tubular function and morphological changes in kidneys of male Wistar rats after 6 months of streptozocin (STZ)-induced diabetes. Diabetic rats had a higher kidney weight/body weight ratio (P<.001), but neither AG nor PB influenced the increased ratio. Diabetes caused an increased urinary albumin excretion (P<.05), which was normalized by AG, but further exaggerated by PB (P<.001). Diabetes also caused an increase in the urinary excretion of Tamm–Horsfall protein (P<.001). Both AG and PB attenuated this increase (P<.05 for both). A few glomeruli displayed focal thickening of varying degrees. Silver staining disclosed the glomerulopathy to be intercapillary glomerulosclerosis. Rats on PB-enriched diet displayed less pronounced changes than untreated rats (P<.01), while AG had no effect. The results suggest that oxidative stress could be involved in the development of diabetic nephropathy. (Less)
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keywords
Free Radical Scavengers: pharmacology, Diabetic Nephropathies: physiopathology, Disease Progression, Experimental: physiopathology, Probucol: pharmacology, Rats, Animal, Diabetes Mellitus, Non-U.S. Gov't, Distal: physiopathology, Male, Oxidative Stress: physiology, Support, Wistar, Kidney Tubules, Distal: pathology, Distal: drug effects, Kidney Glomerulus: physiopathology, Kidney Glomerulus: pathology, Kidney Glomerulus: drug effects, Guanidines: pharmacology, Advanced: antagonists & inhibitors, Glycosylation End Products, Glycosylation
in
Journal of Diabetes and its Complications
volume
16
issue
6
pages
395 - 400
publisher
Elsevier
external identifiers
  • wos:000179378700006
  • scopus:0036860156
ISSN
1873-460X
DOI
10.1016/S1056-8727(02)00164-2
language
English
LU publication?
yes
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The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medicine (Lund) (013230025), Ophthalmology (013242810), Pathology, (Lund) (013030000), Endocrinology (013241500)
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bb1bd286-a922-4d53-ac69-57e9c4bf82d2 (old id 114635)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12477624&dopt=Abstract
date added to LUP
2016-04-01 15:48:21
date last changed
2020-05-26 03:16:10
@article{bb1bd286-a922-4d53-ac69-57e9c4bf82d2,
  abstract     = {We investigated whether aminoguanidine (AG), an inhibitor of advanced glycated end product formation, or probucol (PB), a free radical scavenger, could influence signs of glomerular and distal tubular function and morphological changes in kidneys of male Wistar rats after 6 months of streptozocin (STZ)-induced diabetes. Diabetic rats had a higher kidney weight/body weight ratio (P&lt;.001), but neither AG nor PB influenced the increased ratio. Diabetes caused an increased urinary albumin excretion (P&lt;.05), which was normalized by AG, but further exaggerated by PB (P&lt;.001). Diabetes also caused an increase in the urinary excretion of Tamm–Horsfall protein (P&lt;.001). Both AG and PB attenuated this increase (P&lt;.05 for both). A few glomeruli displayed focal thickening of varying degrees. Silver staining disclosed the glomerulopathy to be intercapillary glomerulosclerosis. Rats on PB-enriched diet displayed less pronounced changes than untreated rats (P&lt;.01), while AG had no effect. The results suggest that oxidative stress could be involved in the development of diabetic nephropathy.},
  author       = {Agardh, Carl David and Stenram, Unne and Torffvit, Ole and Agardh, Elisabet},
  issn         = {1873-460X},
  language     = {eng},
  number       = {6},
  pages        = {395--400},
  publisher    = {Elsevier},
  series       = {Journal of Diabetes and its Complications},
  title        = {Effects of inhibition of glycation and oxidative stress on the development of diabetic nephropathy in rats.},
  url          = {http://dx.doi.org/10.1016/S1056-8727(02)00164-2},
  doi          = {10.1016/S1056-8727(02)00164-2},
  volume       = {16},
  year         = {2002},
}