Advanced

Effects of epithelial and neutrophil CXCR2 on innate immunity and resistance to kidney infection.

Svensson, Majlis LU ; Irjala, Heikki LU ; Svanborg, Catharina LU and Godaly, Gabriela LU (2008) In Kidney International 74. p.81-90
Abstract
The murine chemokine receptor 2 (mCXCR2) controls resistance to urinary tract infection. We have previously shown that mCXCR2 knockout mice develop severe acute pyelonephritis and renal tissue damage with sub-epithelial neutrophil entrapment. In this study we examined the relative importance of neutrophil- and epithelial-specific mCXCR2 expression for bacterial clearance in bone marrow chimeric mice infected with uropathogenic Escherichia coli. Mice expressing mCXCR2 on their neutrophils responded rapidly to experimental urinary tract infection, clearing the infection from the kidneys. Mice lacking epithelial mCXCR2, however, showed delayed exit of neutrophils from the tissues. Mice lacking neutrophil mCXCR2 and mice with no mCXCR2 had no... (More)
The murine chemokine receptor 2 (mCXCR2) controls resistance to urinary tract infection. We have previously shown that mCXCR2 knockout mice develop severe acute pyelonephritis and renal tissue damage with sub-epithelial neutrophil entrapment. In this study we examined the relative importance of neutrophil- and epithelial-specific mCXCR2 expression for bacterial clearance in bone marrow chimeric mice infected with uropathogenic Escherichia coli. Mice expressing mCXCR2 on their neutrophils responded rapidly to experimental urinary tract infection, clearing the infection from the kidneys. Mice lacking epithelial mCXCR2, however, showed delayed exit of neutrophils from the tissues. Mice lacking neutrophil mCXCR2 and mice with no mCXCR2 had no neutrophil recruitment and bacterial clearance. Mice expressing mCXCR2 only in their epithelial cells had a transient epithelial chemokine response; however, neutrophil recruitment was inhibited and bacteria grew without constraint. Our study shows that the expression of mCXCR2 on hematopoietic cells was crucial for bacterial clearance, while its expression on non-bone marrow-derived cells influenced the neutrophil response. These results emphasize the importance of mCXCR2 for the innate defense against urinary tract infection.Kidney International advance online publication, 9 April 2008; doi:10.1038/ki.2008.105. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Kidney International
volume
74
pages
81 - 90
publisher
Nature Publishing Group
external identifiers
  • WOS:000256788900012
  • PMID:18401338
  • Scopus:45249087678
ISSN
1523-1755
DOI
10.1038/ki.2008.105
language
English
LU publication?
yes
id
e0c02811-5bd9-45fc-9780-eaac96722aa8 (old id 1147550)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18401338?dopt=Abstract
date added to LUP
2008-05-07 13:44:16
date last changed
2017-02-12 04:21:26
@article{e0c02811-5bd9-45fc-9780-eaac96722aa8,
  abstract     = {The murine chemokine receptor 2 (mCXCR2) controls resistance to urinary tract infection. We have previously shown that mCXCR2 knockout mice develop severe acute pyelonephritis and renal tissue damage with sub-epithelial neutrophil entrapment. In this study we examined the relative importance of neutrophil- and epithelial-specific mCXCR2 expression for bacterial clearance in bone marrow chimeric mice infected with uropathogenic Escherichia coli. Mice expressing mCXCR2 on their neutrophils responded rapidly to experimental urinary tract infection, clearing the infection from the kidneys. Mice lacking epithelial mCXCR2, however, showed delayed exit of neutrophils from the tissues. Mice lacking neutrophil mCXCR2 and mice with no mCXCR2 had no neutrophil recruitment and bacterial clearance. Mice expressing mCXCR2 only in their epithelial cells had a transient epithelial chemokine response; however, neutrophil recruitment was inhibited and bacteria grew without constraint. Our study shows that the expression of mCXCR2 on hematopoietic cells was crucial for bacterial clearance, while its expression on non-bone marrow-derived cells influenced the neutrophil response. These results emphasize the importance of mCXCR2 for the innate defense against urinary tract infection.Kidney International advance online publication, 9 April 2008; doi:10.1038/ki.2008.105.},
  author       = {Svensson, Majlis and Irjala, Heikki and Svanborg, Catharina and Godaly, Gabriela},
  issn         = {1523-1755},
  language     = {eng},
  pages        = {81--90},
  publisher    = {Nature Publishing Group},
  series       = {Kidney International},
  title        = {Effects of epithelial and neutrophil CXCR2 on innate immunity and resistance to kidney infection.},
  url          = {http://dx.doi.org/10.1038/ki.2008.105},
  volume       = {74},
  year         = {2008},
}