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Comprehensive evaluation of genetic variation in S100A7 suggests an association with the occurrence of allergic rhinitis.

Bryborn, Malin LU ; Halldén, Christer LU ; Säll, Torbjörn LU ; Adner, Mikael LU and Cardell, Lars-Olaf LU (2008) In Respiratory Research 9(Mar 28).
Abstract
BACKGROUND: S100A7 is a calcium-binding protein with chemotactic and antimicrobial properties. S100A7 protein levels are decreased in nasal lavage fluid from individuals with ongoing allergic rhinitis, suggesting a role for S100A7 in allergic airway inflammation. The aims of this study were to describe genetic variation in S100A7 and search for associations between this variation and allergic rhinitis. METHODS: Peripheral blood was collected from 184 atopic patients with a history of pollen-induced allergic rhinitis and 378 non-atopic individuals, all of Swedish origin. DNA was extracted and the S100A7 gene was resequenced in a subset of 47 randomly selected atopic individuals. Nine polymorphisms were genotyped in 184 atopic and 378... (More)
BACKGROUND: S100A7 is a calcium-binding protein with chemotactic and antimicrobial properties. S100A7 protein levels are decreased in nasal lavage fluid from individuals with ongoing allergic rhinitis, suggesting a role for S100A7 in allergic airway inflammation. The aims of this study were to describe genetic variation in S100A7 and search for associations between this variation and allergic rhinitis. METHODS: Peripheral blood was collected from 184 atopic patients with a history of pollen-induced allergic rhinitis and 378 non-atopic individuals, all of Swedish origin. DNA was extracted and the S100A7 gene was resequenced in a subset of 47 randomly selected atopic individuals. Nine polymorphisms were genotyped in 184 atopic and 378 non-atopic individuals and subsequently investigated for associations with allergic rhinitis as well as skin prick test results. Haplotypes were estimated and compared in the two groups. RESULTS: Thirteen polymorphisms were identified in S100A7, of which 7 were previously undescribed. rs3014837 (G/C), which gives rise to an Asp --> Glu amino acid shift, had significantly increased minor allele frequency in atopic individuals. The major haplotype, containing the major allele at all sites, was more common in non-atopic individuals, while the haplotype containing the minor allele at rs3014837 was equally more common among the atopic individuals. Additionally, heterozygotes at this site had significantly higher scores in skin prick tests for 9 out of 11 tested allergens, compared to homozygotes. CONCLUSION: This is the first study describing genetic variation, associated with allergy, in S100A7. The results indicate that rs3014837 is linked to allergic rhinitis in our Swedish population and render S100A7 a strong candidate for further investigations regarding its role in allergic inflammation. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Respiratory Research
volume
9
issue
Mar 28
publisher
BioMed Central
external identifiers
  • wos:000255478800001
  • scopus:43549126463
ISSN
1465-9921
DOI
10.1186/1465-9921-9-29
language
English
LU publication?
yes
id
f3f2acd3-0e61-43df-a5f6-5210747d8f62 (old id 1147982)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18373864?dopt=Abstract
date added to LUP
2008-05-08 09:17:20
date last changed
2017-01-01 07:30:47
@article{f3f2acd3-0e61-43df-a5f6-5210747d8f62,
  abstract     = {BACKGROUND: S100A7 is a calcium-binding protein with chemotactic and antimicrobial properties. S100A7 protein levels are decreased in nasal lavage fluid from individuals with ongoing allergic rhinitis, suggesting a role for S100A7 in allergic airway inflammation. The aims of this study were to describe genetic variation in S100A7 and search for associations between this variation and allergic rhinitis. METHODS: Peripheral blood was collected from 184 atopic patients with a history of pollen-induced allergic rhinitis and 378 non-atopic individuals, all of Swedish origin. DNA was extracted and the S100A7 gene was resequenced in a subset of 47 randomly selected atopic individuals. Nine polymorphisms were genotyped in 184 atopic and 378 non-atopic individuals and subsequently investigated for associations with allergic rhinitis as well as skin prick test results. Haplotypes were estimated and compared in the two groups. RESULTS: Thirteen polymorphisms were identified in S100A7, of which 7 were previously undescribed. rs3014837 (G/C), which gives rise to an Asp --> Glu amino acid shift, had significantly increased minor allele frequency in atopic individuals. The major haplotype, containing the major allele at all sites, was more common in non-atopic individuals, while the haplotype containing the minor allele at rs3014837 was equally more common among the atopic individuals. Additionally, heterozygotes at this site had significantly higher scores in skin prick tests for 9 out of 11 tested allergens, compared to homozygotes. CONCLUSION: This is the first study describing genetic variation, associated with allergy, in S100A7. The results indicate that rs3014837 is linked to allergic rhinitis in our Swedish population and render S100A7 a strong candidate for further investigations regarding its role in allergic inflammation.},
  articleno    = {29},
  author       = {Bryborn, Malin and Halldén, Christer and Säll, Torbjörn and Adner, Mikael and Cardell, Lars-Olaf},
  issn         = {1465-9921},
  language     = {eng},
  number       = {Mar 28},
  publisher    = {BioMed Central},
  series       = {Respiratory Research},
  title        = {Comprehensive evaluation of genetic variation in S100A7 suggests an association with the occurrence of allergic rhinitis.},
  url          = {http://dx.doi.org/10.1186/1465-9921-9-29},
  volume       = {9},
  year         = {2008},
}