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High tumor mast cell density is associated with longer survival of colon cancer patients

Mehdawi, Lubna LU ; Osman, Janina LU ; Topi, Geriolda LU and Sjölander, Anita LU (2016) In Acta Oncologica 55(12). p.1434-1442
Abstract

Background: Inflammatory cells and inflammatory mediators play an important role in colorectal cancer (CRC). Previous studies have shown that CRC patients with increased expression of cysteinyl leukotriene receptor 1 (CysLTR1) have a poorer prognosis, and Cysltr1−/− mice display fewer intestinal polyps. However, the role of mast cells (MCs) in colon cancer progression remains unclear. The aim of the present study was to explore the relevance of MCs in CRC. Material and methods: A tissue microarray from 72 CRC patients was stained with MC anti-tryptase and -chymase antibodies. Mouse colon tissue was stained with MC anti-tryptase antibody. Immunohistochemistry was used to identify MCs in patients and mice. Results: Patient... (More)

Background: Inflammatory cells and inflammatory mediators play an important role in colorectal cancer (CRC). Previous studies have shown that CRC patients with increased expression of cysteinyl leukotriene receptor 1 (CysLTR1) have a poorer prognosis, and Cysltr1−/− mice display fewer intestinal polyps. However, the role of mast cells (MCs) in colon cancer progression remains unclear. The aim of the present study was to explore the relevance of MCs in CRC. Material and methods: A tissue microarray from 72 CRC patients was stained with MC anti-tryptase and -chymase antibodies. Mouse colon tissue was stained with MC anti-tryptase antibody. Immunohistochemistry was used to identify MCs in patients and mice. Results: Patient colon cancer tissue had in comparison with normal colon tissue a reduced number of MCs, predominantly of chymase-positive cells. Further analysis revealed that patients with a relative high MCD in their cancer tissues showed significantly longer overall survival compared to those with a low MCD [hazard ratio (HR) 0.539; 95% confidence interval (CI), 0.302–0.961]. Similar results were observed in subgroups of patients with either no distant metastasis (p = 0.004), or −/− mice showed significantly higher MCs in their polyp/tumor areas compared with wild-type mice. Conclusion: A high MCD in cancer tissue correlated with longer patient survival independently from other risk factors for CRC. The concept that MCs have an anti-tumor effect in CRC is further supported by the findings of a negative correlation with CysLTR1 expression in patients and a high MCD in colon polyps/tumors from CysLTR1−/− mice.

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organization
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type
Contribution to journal
publication status
published
subject
in
Acta Oncologica
volume
55
issue
12
pages
1434 - 1442
publisher
Taylor & Francis
external identifiers
  • scopus:84976338419
ISSN
0284-186X
DOI
10.1080/0284186X.2016.1198493
language
English
LU publication?
yes
id
1149c10d-4ef8-4f41-9e46-512bb27e7636
date added to LUP
2016-07-18 09:50:00
date last changed
2017-04-09 04:47:41
@article{1149c10d-4ef8-4f41-9e46-512bb27e7636,
  abstract     = {<p>Background: Inflammatory cells and inflammatory mediators play an important role in colorectal cancer (CRC). Previous studies have shown that CRC patients with increased expression of cysteinyl leukotriene receptor 1 (CysLTR1) have a poorer prognosis, and Cysltr1<sup>−/−</sup> mice display fewer intestinal polyps. However, the role of mast cells (MCs) in colon cancer progression remains unclear. The aim of the present study was to explore the relevance of MCs in CRC. Material and methods: A tissue microarray from 72 CRC patients was stained with MC anti-tryptase and -chymase antibodies. Mouse colon tissue was stained with MC anti-tryptase antibody. Immunohistochemistry was used to identify MCs in patients and mice. Results: Patient colon cancer tissue had in comparison with normal colon tissue a reduced number of MCs, predominantly of chymase-positive cells. Further analysis revealed that patients with a relative high MCD in their cancer tissues showed significantly longer overall survival compared to those with a low MCD [hazard ratio (HR) 0.539; 95% confidence interval (CI), 0.302–0.961]. Similar results were observed in subgroups of patients with either no distant metastasis (p = 0.004), or −/− mice showed significantly higher MCs in their polyp/tumor areas compared with wild-type mice. Conclusion: A high MCD in cancer tissue correlated with longer patient survival independently from other risk factors for CRC. The concept that MCs have an anti-tumor effect in CRC is further supported by the findings of a negative correlation with CysLTR1 expression in patients and a high MCD in colon polyps/tumors from CysLTR1<sup>−/−</sup> mice.</p>},
  author       = {Mehdawi, Lubna and Osman, Janina and Topi, Geriolda and Sjölander, Anita},
  issn         = {0284-186X},
  language     = {eng},
  number       = {12},
  pages        = {1434--1442},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {High tumor mast cell density is associated with longer survival of colon cancer patients},
  url          = {http://dx.doi.org/10.1080/0284186X.2016.1198493},
  volume       = {55},
  year         = {2016},
}