INCREASED LEVELS OF GLYCOSAMINOGLYCANS DURING SEPTIC SHOCK: RELATION TO MORTALITY AND THE ANTIBACTERIAL ACTIONS OF PLASMA.
(2008) In Shock 30. p.623-627- Abstract
- Glycosaminoglycans (GAGs) are structurally heterogeneous negatively charged polysaccharides. Endothelial GAGs, also known as glycocalyx, are involved in capillary permeability. In rat venules stimulated with proinflammatory substances ex vivo, the GAG-containing proteoglycan, syndecan-1, is shed from the endothelium. We wanted to investigate if we could trace the same response during septic shock as reflected in the circulating GAG levels. Arterial plasma samples were collected from 18 consecutive septic shock patients admitted to our intensive care unit. Plasma GAGs were measured with an Alcian blue slot binding assay, and syndecan-1 levels were measured with enzyme-linked immunosorbent assay. Effects of GAGs on the antibacterial activity... (More)
- Glycosaminoglycans (GAGs) are structurally heterogeneous negatively charged polysaccharides. Endothelial GAGs, also known as glycocalyx, are involved in capillary permeability. In rat venules stimulated with proinflammatory substances ex vivo, the GAG-containing proteoglycan, syndecan-1, is shed from the endothelium. We wanted to investigate if we could trace the same response during septic shock as reflected in the circulating GAG levels. Arterial plasma samples were collected from 18 consecutive septic shock patients admitted to our intensive care unit. Plasma GAGs were measured with an Alcian blue slot binding assay, and syndecan-1 levels were measured with enzyme-linked immunosorbent assay. Effects of GAGs on the antibacterial activity of plasma were assessed by a radial diffusion assay. The median plasma GAG level was significantly higher in the septic shock patients than in matched controls (median [interquartile range], 2.7 mug/mL [1.9 - 4.8 mug/mL] vs. 1.8 mug/mL [1.7 - 2.0 mug/mL]). Furthermore, the GAG levels were significantly higher in nonsurvivors (4.6 mug/mL [3.1 - 8.8 mug/mL], n = 8) than survivors (1.8 mug/mL [1.6 - 2.6 mug/mL], n = 10). The syndecan-1 levels were also increased in the patients compared with controls (246 ng/mL [180 - 496 ng/mL] vs. 26 ng/mL [23 - 31 ng/mL]) and correlated to the cardiovascular Sequential Organ Failure Assessment score. The GAGs inhibited the endogenous antibacterial activity of plasma as well as isolated antimicrobial peptides. The concentrations required were in the same range as the GAG levels measured in the patients. These results show that the GAG levels are increased in septic shock patients, possibly reflecting peripheral endothelial cell damage. We also found that GAGs in relevant concentrations neutralize antimicrobial peptides in plasma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1153827
- author
- Nelson, Axel ; Berkestedt, Ingrid LU ; Schmidtchen, Artur LU ; Ljunggren, Lennart and Bodelsson, Mikael LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Shock
- volume
- 30
- pages
- 623 - 627
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000261032600003
- pmid:18497712
- scopus:54849433850
- ISSN
- 1540-0514
- DOI
- 10.1097/SHK.0b013e3181777da3
- language
- English
- LU publication?
- yes
- id
- 3eb1490b-2ef0-4039-9916-1cdc380aefac (old id 1153827)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18497712?dopt=Abstract
- date added to LUP
- 2016-04-04 07:31:21
- date last changed
- 2024-02-10 21:11:31
@article{3eb1490b-2ef0-4039-9916-1cdc380aefac,
abstract = {{Glycosaminoglycans (GAGs) are structurally heterogeneous negatively charged polysaccharides. Endothelial GAGs, also known as glycocalyx, are involved in capillary permeability. In rat venules stimulated with proinflammatory substances ex vivo, the GAG-containing proteoglycan, syndecan-1, is shed from the endothelium. We wanted to investigate if we could trace the same response during septic shock as reflected in the circulating GAG levels. Arterial plasma samples were collected from 18 consecutive septic shock patients admitted to our intensive care unit. Plasma GAGs were measured with an Alcian blue slot binding assay, and syndecan-1 levels were measured with enzyme-linked immunosorbent assay. Effects of GAGs on the antibacterial activity of plasma were assessed by a radial diffusion assay. The median plasma GAG level was significantly higher in the septic shock patients than in matched controls (median [interquartile range], 2.7 mug/mL [1.9 - 4.8 mug/mL] vs. 1.8 mug/mL [1.7 - 2.0 mug/mL]). Furthermore, the GAG levels were significantly higher in nonsurvivors (4.6 mug/mL [3.1 - 8.8 mug/mL], n = 8) than survivors (1.8 mug/mL [1.6 - 2.6 mug/mL], n = 10). The syndecan-1 levels were also increased in the patients compared with controls (246 ng/mL [180 - 496 ng/mL] vs. 26 ng/mL [23 - 31 ng/mL]) and correlated to the cardiovascular Sequential Organ Failure Assessment score. The GAGs inhibited the endogenous antibacterial activity of plasma as well as isolated antimicrobial peptides. The concentrations required were in the same range as the GAG levels measured in the patients. These results show that the GAG levels are increased in septic shock patients, possibly reflecting peripheral endothelial cell damage. We also found that GAGs in relevant concentrations neutralize antimicrobial peptides in plasma.}},
author = {{Nelson, Axel and Berkestedt, Ingrid and Schmidtchen, Artur and Ljunggren, Lennart and Bodelsson, Mikael}},
issn = {{1540-0514}},
language = {{eng}},
pages = {{623--627}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Shock}},
title = {{INCREASED LEVELS OF GLYCOSAMINOGLYCANS DURING SEPTIC SHOCK: RELATION TO MORTALITY AND THE ANTIBACTERIAL ACTIONS OF PLASMA.}},
url = {{http://dx.doi.org/10.1097/SHK.0b013e3181777da3}},
doi = {{10.1097/SHK.0b013e3181777da3}},
volume = {{30}},
year = {{2008}},
}