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Ectomycorrhizal mycelial species composition in apatite amended and non-amended mesh bags buried in a phosphorus-poor spruce forest.

Hedh, Jenny LU ; Wallander, Håkan LU and Erland, Susanne LU (2008) In Mycological Research 112(6). p.681-688
Abstract
We studied the effect of apatite amendment on ectomycorrhizal (EM) mycelial biomass production and species composition in a phosphorus-poor spruce forest using sand-filled mesh bags. Control and apatite-amended bags were buried in pairs in the lower part of the organic horizon for one growth season. DNA extraction, PCR of the ITS region, cloning and random sequencing were used to examine the fungal species composition in each bag. Sequences were identified by comparison with the UNITE database and GenBank. Our study confirmed previous results that the major fungal ingrowth in mesh bags was of EM origin. On average 13 species were detected in each bag. Tylospora fibrillosa, Amphinema sp., Tomentellopsis submollis, and Xerocomus badius made... (More)
We studied the effect of apatite amendment on ectomycorrhizal (EM) mycelial biomass production and species composition in a phosphorus-poor spruce forest using sand-filled mesh bags. Control and apatite-amended bags were buried in pairs in the lower part of the organic horizon for one growth season. DNA extraction, PCR of the ITS region, cloning and random sequencing were used to examine the fungal species composition in each bag. Sequences were identified by comparison with the UNITE database and GenBank. Our study confirmed previous results that the major fungal ingrowth in mesh bags was of EM origin. On average 13 species were detected in each bag. Tylospora fibrillosa, Amphinema sp., Tomentellopsis submollis, and Xerocomus badius made up almost 80% of the EM sequences. High biomass was related to increased dominance of specific species. There were no statistically significant differences in biomass production estimated from PLFA 18:2omega6, 9, or between fungal communities of apatite-amended and control bags estimated from DNA after one growth season. The potential of the mesh bag method in studies of functional diversity of EM mycelia in the field is discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research
volume
112
issue
6
pages
681 - 688
publisher
Cambridge University Press
external identifiers
  • wos:000257532600006
  • scopus:44649123590
ISSN
1469-8102
DOI
10.1016/j.mycres.2007.11.008
project
Ectomycorrhizal fungi and apatite weathering
language
English
LU publication?
yes
id
9b523110-2b03-4190-8dc0-e33eb3d771da (old id 1153872)
date added to LUP
2008-11-06 12:34:50
date last changed
2017-09-10 03:48:47
@article{9b523110-2b03-4190-8dc0-e33eb3d771da,
  abstract     = {We studied the effect of apatite amendment on ectomycorrhizal (EM) mycelial biomass production and species composition in a phosphorus-poor spruce forest using sand-filled mesh bags. Control and apatite-amended bags were buried in pairs in the lower part of the organic horizon for one growth season. DNA extraction, PCR of the ITS region, cloning and random sequencing were used to examine the fungal species composition in each bag. Sequences were identified by comparison with the UNITE database and GenBank. Our study confirmed previous results that the major fungal ingrowth in mesh bags was of EM origin. On average 13 species were detected in each bag. Tylospora fibrillosa, Amphinema sp., Tomentellopsis submollis, and Xerocomus badius made up almost 80% of the EM sequences. High biomass was related to increased dominance of specific species. There were no statistically significant differences in biomass production estimated from PLFA 18:2omega6, 9, or between fungal communities of apatite-amended and control bags estimated from DNA after one growth season. The potential of the mesh bag method in studies of functional diversity of EM mycelia in the field is discussed.},
  author       = {Hedh, Jenny and Wallander, Håkan and Erland, Susanne},
  issn         = {1469-8102},
  language     = {eng},
  number       = {6},
  pages        = {681--688},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {Ectomycorrhizal mycelial species composition in apatite amended and non-amended mesh bags buried in a phosphorus-poor spruce forest.},
  url          = {http://dx.doi.org/10.1016/j.mycres.2007.11.008},
  volume       = {112},
  year         = {2008},
}