Deciphering developmental stages of adult myelopoiesis.
(2008) In Cell Cycle 7(6). p.706-713- Abstract
- The ability to subfractionate minor cellular subsets by multiparameter flow cytometry and to evaluate such cells for functional properties has been used to ascertain lineal relationships and detail developmental hierarchies in the hematopoietic system for more than 20 years. However, steady advances in technology combined with the use of novel cell surface markers continues to redefine the developmental landscape as novel subpopulations are purified and characterized. We recently used such an approach to stage progenitor cell hierarchy involved in myeloid development with the use of two markers, Slamf1 and Endoglin that have recently been shown to be associated with hematopoietic stem cells. Here, we provide additional characterization of... (More)
- The ability to subfractionate minor cellular subsets by multiparameter flow cytometry and to evaluate such cells for functional properties has been used to ascertain lineal relationships and detail developmental hierarchies in the hematopoietic system for more than 20 years. However, steady advances in technology combined with the use of novel cell surface markers continues to redefine the developmental landscape as novel subpopulations are purified and characterized. We recently used such an approach to stage progenitor cell hierarchy involved in myeloid development with the use of two markers, Slamf1 and Endoglin that have recently been shown to be associated with hematopoietic stem cells. Here, we provide additional characterization of these cellular subsets to further refine their developmental potential. Little or no alterations in lineage potential were observed in these subsets when evaluated in a BCL2 transgenic setting or in response to various growth factor combinations, although BCL2 significantly enhanced their in vitro readout. Gene expression patterns of functionally opposing transcription factors that are known to play key roles for the appropriate development into separate myeloid lineages were associated with the functional activity of prospectively isolated subsets. Multiple genes traditionally associated with early lymphopoiesis were observed in early candidate granulocyte/monocyte, but not early megakaryocytic and/or erythroid progenitor cells. When functionally evaluated, such early granulocyte/monocyte precursors displayed a latent lymphoid activity, which was pronounced in subsets bearing high expression of the tyrosine kinase receptor FLT3. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1154495
- author
- Pronk, Cornelis J ; Attema, Joanne LU ; Rossi, Derrick J ; Sigvardsson, Mikael LU and Bryder, David LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Cycle
- volume
- 7
- issue
- 6
- pages
- 706 - 713
- publisher
- Landes Bioscience
- external identifiers
-
- wos:000254771400004
- pmid:18461720
- scopus:42049120265
- ISSN
- 1551-4005
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020)
- id
- 3b91b184-b1c2-4018-9b8d-875924ba1bdf (old id 1154495)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18461720?dopt=Abstract
- date added to LUP
- 2016-04-04 07:40:21
- date last changed
- 2022-01-29 02:27:26
@article{3b91b184-b1c2-4018-9b8d-875924ba1bdf,
abstract = {{The ability to subfractionate minor cellular subsets by multiparameter flow cytometry and to evaluate such cells for functional properties has been used to ascertain lineal relationships and detail developmental hierarchies in the hematopoietic system for more than 20 years. However, steady advances in technology combined with the use of novel cell surface markers continues to redefine the developmental landscape as novel subpopulations are purified and characterized. We recently used such an approach to stage progenitor cell hierarchy involved in myeloid development with the use of two markers, Slamf1 and Endoglin that have recently been shown to be associated with hematopoietic stem cells. Here, we provide additional characterization of these cellular subsets to further refine their developmental potential. Little or no alterations in lineage potential were observed in these subsets when evaluated in a BCL2 transgenic setting or in response to various growth factor combinations, although BCL2 significantly enhanced their in vitro readout. Gene expression patterns of functionally opposing transcription factors that are known to play key roles for the appropriate development into separate myeloid lineages were associated with the functional activity of prospectively isolated subsets. Multiple genes traditionally associated with early lymphopoiesis were observed in early candidate granulocyte/monocyte, but not early megakaryocytic and/or erythroid progenitor cells. When functionally evaluated, such early granulocyte/monocyte precursors displayed a latent lymphoid activity, which was pronounced in subsets bearing high expression of the tyrosine kinase receptor FLT3.}},
author = {{Pronk, Cornelis J and Attema, Joanne and Rossi, Derrick J and Sigvardsson, Mikael and Bryder, David}},
issn = {{1551-4005}},
language = {{eng}},
number = {{6}},
pages = {{706--713}},
publisher = {{Landes Bioscience}},
series = {{Cell Cycle}},
title = {{Deciphering developmental stages of adult myelopoiesis.}},
url = {{http://www.ncbi.nlm.nih.gov/pubmed/18461720?dopt=Abstract}},
volume = {{7}},
year = {{2008}},
}