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Different cell cycle kinetic effects of N1,N11-diethylnorspermine-induced polyamine depletion in four human breast cancer cell lines.

Myhre, Louise LU ; Alm, Kersti LU ; Hegardt, Cecilia LU ; Staaf, Johan LU ; Jönsson, Göran B LU ; Larsson, Sara LU and Oredsson, Stina LU (2008) In Anti-Cancer Drugs 19(4). p.359-368
Abstract
Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a... (More)
Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a number of cell cycle regulatory proteins such as cyclin E1, cyclin A2, and cyclin B1 were lowered by DENSPM treatment, which may explain the effects on cell cycle kinetics. The two cell lines that were most sensitive to DENSPM treatment belong to the basal-like subtype of breast cancer and they were deficient with respect to p53, BRCA1, and RB1. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Anti-Cancer Drugs
volume
19
issue
4
pages
359 - 368
publisher
Rapid Communications
external identifiers
  • wos:000254247000004
  • pmid:18454046
  • scopus:47949093670
ISSN
0959-4973
DOI
10.1097/CAD.0b013e3282f7f518
language
English
LU publication?
yes
id
3485bb93-05ae-4570-9523-e00b49cb3c0c (old id 1154588)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18454046?dopt=Abstract
date added to LUP
2008-06-04 12:11:44
date last changed
2017-10-08 04:30:16
@article{3485bb93-05ae-4570-9523-e00b49cb3c0c,
  abstract     = {Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a number of cell cycle regulatory proteins such as cyclin E1, cyclin A2, and cyclin B1 were lowered by DENSPM treatment, which may explain the effects on cell cycle kinetics. The two cell lines that were most sensitive to DENSPM treatment belong to the basal-like subtype of breast cancer and they were deficient with respect to p53, BRCA1, and RB1.},
  author       = {Myhre, Louise and Alm, Kersti and Hegardt, Cecilia and Staaf, Johan and Jönsson, Göran B and Larsson, Sara and Oredsson, Stina},
  issn         = {0959-4973},
  language     = {eng},
  number       = {4},
  pages        = {359--368},
  publisher    = {Rapid Communications},
  series       = {Anti-Cancer Drugs},
  title        = {Different cell cycle kinetic effects of N1,N11-diethylnorspermine-induced polyamine depletion in four human breast cancer cell lines.},
  url          = {http://dx.doi.org/10.1097/CAD.0b013e3282f7f518},
  volume       = {19},
  year         = {2008},
}