Cytokine repertoire of epithelial cells lining the human urinary tract
Hang, L LU ; Wullt, B LU ; Shen, Z LU ; Karpman, D LU
and Svanborg, C
LU
(1998)
In The Journal of urology
159(6).
p.92-2185
- Abstract
PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.
MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.
RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in... (More)
PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.
MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.
RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in disease-free tissues and sections with cancer or interstitial cystitis (IC). In contrast, staining for IL-1beta, IL-4, IL-6 and IFNgamma varied with the disease state of the patient. Epithelial IL-1beta staining was absent (0%) in sections from healthy bladder, but positive in tissues with IC or cancer-associated pathology (50 to 100%). IL-6 staining was detected in the epithelial layer of several patients with IC or cancer related pathology, but only in cells with non-epithelial morphology and not in disease-free tissues. IFNgamma and IL-4 staining were only observed in patients with IC and only in cells with non-epithelial morphology.
CONCLUSIONS: The results show that epithelial cells from all parts of the urinary tract constitutively produce IL-8 and TGFbeta and suggest that the production of other cytokines varies with the disease of the patient. Constitutive cytokine production provides the basis for a rapid host response, in the defense against mucosal attack by microbes or toxic agents.
(Less)
- author
- Hang, L
LU
; Wullt, B
LU
; Shen, Z
LU
; Karpman, D
LU
and Svanborg, C
LU
- organization
- publishing date
- 1998-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adult, Aged, Antibodies, Monoclonal, Cytokines, Epithelial Cells, Female, Humans, Immunohistochemistry, Interleukin-8, Male, Middle Aged, Urinary Tract, Urologic Neoplasms, Journal Article, Research Support, Non-U.S. Gov't
- in
- The Journal of urology
- volume
- 159
- issue
- 6
- pages
- 8 pages
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:9598567
- scopus:0032431731
- ISSN
- 0022-5347
- DOI
- 10.1016/S0022-5347(01)63303-2
- language
- English
- LU publication?
- yes
- id
- 11548123-d3b5-45f2-babb-9fd8705b31b2
- date added to LUP
- 2017-02-08 16:02:03
- date last changed
- 2024-05-26 08:56:16
@article{11548123-d3b5-45f2-babb-9fd8705b31b2,
abstract = {{<p>PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.</p><p>MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.</p><p>RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in disease-free tissues and sections with cancer or interstitial cystitis (IC). In contrast, staining for IL-1beta, IL-4, IL-6 and IFNgamma varied with the disease state of the patient. Epithelial IL-1beta staining was absent (0%) in sections from healthy bladder, but positive in tissues with IC or cancer-associated pathology (50 to 100%). IL-6 staining was detected in the epithelial layer of several patients with IC or cancer related pathology, but only in cells with non-epithelial morphology and not in disease-free tissues. IFNgamma and IL-4 staining were only observed in patients with IC and only in cells with non-epithelial morphology.</p><p>CONCLUSIONS: The results show that epithelial cells from all parts of the urinary tract constitutively produce IL-8 and TGFbeta and suggest that the production of other cytokines varies with the disease of the patient. Constitutive cytokine production provides the basis for a rapid host response, in the defense against mucosal attack by microbes or toxic agents.</p>}},
author = {{Hang, L and Wullt, B and Shen, Z and Karpman, D and Svanborg, C}},
issn = {{0022-5347}},
keywords = {{Adult; Aged; Antibodies, Monoclonal; Cytokines; Epithelial Cells; Female; Humans; Immunohistochemistry; Interleukin-8; Male; Middle Aged; Urinary Tract; Urologic Neoplasms; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{6}},
pages = {{92--2185}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{The Journal of urology}},
title = {{Cytokine repertoire of epithelial cells lining the human urinary tract}},
url = {{http://dx.doi.org/10.1016/S0022-5347(01)63303-2}},
doi = {{10.1016/S0022-5347(01)63303-2}},
volume = {{159}},
year = {{1998}},
}