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Cytokine repertoire of epithelial cells lining the human urinary tract

Hang, L LU ; Wullt, B LU ; Shen, Z LU ; Karpman, D LU and Svanborg, C LU (1998) In The Journal of urology 159(6). p.92-2185
Abstract

PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.

MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.

RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in... (More)

PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.

MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.

RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in disease-free tissues and sections with cancer or interstitial cystitis (IC). In contrast, staining for IL-1beta, IL-4, IL-6 and IFNgamma varied with the disease state of the patient. Epithelial IL-1beta staining was absent (0%) in sections from healthy bladder, but positive in tissues with IC or cancer-associated pathology (50 to 100%). IL-6 staining was detected in the epithelial layer of several patients with IC or cancer related pathology, but only in cells with non-epithelial morphology and not in disease-free tissues. IFNgamma and IL-4 staining were only observed in patients with IC and only in cells with non-epithelial morphology.

CONCLUSIONS: The results show that epithelial cells from all parts of the urinary tract constitutively produce IL-8 and TGFbeta and suggest that the production of other cytokines varies with the disease of the patient. Constitutive cytokine production provides the basis for a rapid host response, in the defense against mucosal attack by microbes or toxic agents.

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published
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keywords
Adult, Aged, Antibodies, Monoclonal, Cytokines, Epithelial Cells, Female, Humans, Immunohistochemistry, Interleukin-8, Male, Middle Aged, Urinary Tract, Urologic Neoplasms, Journal Article, Research Support, Non-U.S. Gov't
in
The Journal of urology
volume
159
issue
6
pages
8 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0032431731
ISSN
0022-5347
DOI
10.1016/S0022-5347(01)63303-2
language
English
LU publication?
yes
id
11548123-d3b5-45f2-babb-9fd8705b31b2
date added to LUP
2017-02-08 16:02:03
date last changed
2017-07-09 04:58:01
@article{11548123-d3b5-45f2-babb-9fd8705b31b2,
  abstract     = {<p>PURPOSE: To examine the cytokine profile of epithelial cells lining the human urinary tract with the aim of differentiating between the constitutive and disease-related cytokine production in these tissues.</p><p>MATERIALS AND METHODS: Sections from the renal pelvis, ureter, bladder or urethra were obtained from 22 patients undergoing urinary tract surgery and were stained with monoclonal antibodies to interleukin(IL)-1beta, IL-4, IL-6, IL-8, interferon gamma (IFNgamma) and transforming growth factor beta (TGFbeta). Sections were classified according to the presence or absence of disease in the tissue.</p><p>RESULTS: Epithelial cells lining the renal pelvis, ureter, bladder or urethra all stained for IL-8 and TGFbeta (100%) in disease-free tissues and sections with cancer or interstitial cystitis (IC). In contrast, staining for IL-1beta, IL-4, IL-6 and IFNgamma varied with the disease state of the patient. Epithelial IL-1beta staining was absent (0%) in sections from healthy bladder, but positive in tissues with IC or cancer-associated pathology (50 to 100%). IL-6 staining was detected in the epithelial layer of several patients with IC or cancer related pathology, but only in cells with non-epithelial morphology and not in disease-free tissues. IFNgamma and IL-4 staining were only observed in patients with IC and only in cells with non-epithelial morphology.</p><p>CONCLUSIONS: The results show that epithelial cells from all parts of the urinary tract constitutively produce IL-8 and TGFbeta and suggest that the production of other cytokines varies with the disease of the patient. Constitutive cytokine production provides the basis for a rapid host response, in the defense against mucosal attack by microbes or toxic agents.</p>},
  author       = {Hang, L and Wullt, B and Shen, Z and Karpman, D and Svanborg, C},
  issn         = {0022-5347},
  keyword      = {Adult,Aged,Antibodies, Monoclonal,Cytokines,Epithelial Cells,Female,Humans,Immunohistochemistry,Interleukin-8,Male,Middle Aged,Urinary Tract,Urologic Neoplasms,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {6},
  pages        = {92--2185},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {The Journal of urology},
  title        = {Cytokine repertoire of epithelial cells lining the human urinary tract},
  url          = {http://dx.doi.org/10.1016/S0022-5347(01)63303-2},
  volume       = {159},
  year         = {1998},
}