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Combined lack of estrogen receptors alpha and beta affects vascular iNOS protein expression.

Liang, Min LU ; Ekblad, Eva LU ; Lydrup, M.-L. and Nilsson, Bengt-Olof LU (2003) In Cell and Tissue Research1974-01-01+01:00 313(1). p.63-70
Abstract
Endothelial and vascular smooth muscle cells express both estrogen receptor (ER) agr and beta. Recent findings indicate that vascular ERbeta and ERagr may substitute for one another. Here, we investigate vascular morphology, contractility and protein expression in intact aorta from adult (4 months old) female mice lacking both ERagr and ERbeta (DERKO). The body weights were 17% higher (P<0.01) in DERKO than in wild-type mice. Vascular morphology, investigated in paraffin sections from aorta stained with hematoxylin-eosin or van Gieson, was identical in DERKO and wild-type mice. Endothelial cells were clearly visible in aorta of both DERKO and wild-type animals. Morphometric analysis of media thickness and wall to lumen ratio using a... (More)
Endothelial and vascular smooth muscle cells express both estrogen receptor (ER) agr and beta. Recent findings indicate that vascular ERbeta and ERagr may substitute for one another. Here, we investigate vascular morphology, contractility and protein expression in intact aorta from adult (4 months old) female mice lacking both ERagr and ERbeta (DERKO). The body weights were 17% higher (P<0.01) in DERKO than in wild-type mice. Vascular morphology, investigated in paraffin sections from aorta stained with hematoxylin-eosin or van Gieson, was identical in DERKO and wild-type mice. Endothelial cells were clearly visible in aorta of both DERKO and wild-type animals. Morphometric analysis of media thickness and wall to lumen ratio using a computerized image analyzing system demonstrated no differences between the two groups of mice. The vascular expression of endothelial nitric oxide synthase (eNOS, NOS III) and inducible nitric oxide synthase (iNOS, NOS II) was investigated using Western blotting. Aorta from both DERKO and wild-type mice expressed iNOS protein, but the iNOS expression was 3 times lower (P<0.05) in DERKO compared to wild-type mice. No difference in eNOS protein level between the two groups of animals was observed. Force responses to noradrenaline, determined either in the absence or in the presence of the nitric oxide synthase inhibitor l-NAME and the cyclo-oxygenase inhibitor indomethacin, were unaffected by the lack of functional ERagr/ERbeta. In summary, combined lack of functional ERagr and ERbeta lowers the vascular expression of iNOS but has no effects on morphology, eNOS expression, and noradrenaline sensitivity in the intact aorta. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aorta, Nitric oxide synthase, Noradrenaline, Vascular morphology, Estrogen receptor knockout, Mouse (DERKO)
in
Cell and Tissue Research1974-01-01+01:00
volume
313
issue
1
pages
63 - 70
publisher
Springer
external identifiers
  • wos:000184839000007
  • pmid:12827494
  • scopus:0041573043
ISSN
1432-0878
DOI
10.1007/s00441-003-0731-3
language
English
LU publication?
yes
id
fa901ff8-bd33-4537-a2fe-41091ec36596 (old id 115652)
date added to LUP
2007-07-16 15:09:26
date last changed
2018-05-29 12:21:00
@article{fa901ff8-bd33-4537-a2fe-41091ec36596,
  abstract     = {Endothelial and vascular smooth muscle cells express both estrogen receptor (ER) agr and beta. Recent findings indicate that vascular ERbeta and ERagr may substitute for one another. Here, we investigate vascular morphology, contractility and protein expression in intact aorta from adult (4 months old) female mice lacking both ERagr and ERbeta (DERKO). The body weights were 17% higher (P&lt;0.01) in DERKO than in wild-type mice. Vascular morphology, investigated in paraffin sections from aorta stained with hematoxylin-eosin or van Gieson, was identical in DERKO and wild-type mice. Endothelial cells were clearly visible in aorta of both DERKO and wild-type animals. Morphometric analysis of media thickness and wall to lumen ratio using a computerized image analyzing system demonstrated no differences between the two groups of mice. The vascular expression of endothelial nitric oxide synthase (eNOS, NOS III) and inducible nitric oxide synthase (iNOS, NOS II) was investigated using Western blotting. Aorta from both DERKO and wild-type mice expressed iNOS protein, but the iNOS expression was 3 times lower (P&lt;0.05) in DERKO compared to wild-type mice. No difference in eNOS protein level between the two groups of animals was observed. Force responses to noradrenaline, determined either in the absence or in the presence of the nitric oxide synthase inhibitor l-NAME and the cyclo-oxygenase inhibitor indomethacin, were unaffected by the lack of functional ERagr/ERbeta. In summary, combined lack of functional ERagr and ERbeta lowers the vascular expression of iNOS but has no effects on morphology, eNOS expression, and noradrenaline sensitivity in the intact aorta.},
  author       = {Liang, Min and Ekblad, Eva and Lydrup, M.-L. and Nilsson, Bengt-Olof},
  issn         = {1432-0878},
  keyword      = {Aorta,Nitric oxide synthase,Noradrenaline,Vascular morphology,Estrogen receptor knockout,Mouse (DERKO)},
  language     = {eng},
  number       = {1},
  pages        = {63--70},
  publisher    = {Springer},
  series       = {Cell and Tissue Research1974-01-01+01:00},
  title        = {Combined lack of estrogen receptors alpha and beta affects vascular iNOS protein expression.},
  url          = {http://dx.doi.org/10.1007/s00441-003-0731-3},
  volume       = {313},
  year         = {2003},
}