A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.
(2003) In European Journal of Biochemistry 270(15). p.3146-3152- Abstract
- Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their... (More)
- Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/116377
- author
- Ramström, Margareta ; Hagman, Charlotte ; Tsybin, Youri O ; Markides, Karin E ; Håkansson, Per ; Salehi, S Albert LU ; Lundquist, Ingmar LU ; Håkanson, Rolf LU and Bergquist, Jonas
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Biochemistry
- volume
- 270
- issue
- 15
- pages
- 3146 - 3152
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000184267300004
- pmid:12869189
- scopus:0042268140
- ISSN
- 0014-2956
- DOI
- 10.1046/j.1432-1033.2003.03690.x
- language
- English
- LU publication?
- yes
- id
- b845f0e7-020b-4f09-8730-3555d2e7c9bf (old id 116377)
- date added to LUP
- 2016-04-01 17:07:16
- date last changed
- 2022-01-29 00:28:28
@article{b845f0e7-020b-4f09-8730-3555d2e7c9bf, abstract = {{Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts.}}, author = {{Ramström, Margareta and Hagman, Charlotte and Tsybin, Youri O and Markides, Karin E and Håkansson, Per and Salehi, S Albert and Lundquist, Ingmar and Håkanson, Rolf and Bergquist, Jonas}}, issn = {{0014-2956}}, language = {{eng}}, number = {{15}}, pages = {{3146--3152}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Biochemistry}}, title = {{A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.}}, url = {{https://lup.lub.lu.se/search/files/4880684/623834.pdf}}, doi = {{10.1046/j.1432-1033.2003.03690.x}}, volume = {{270}}, year = {{2003}}, }