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A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.

Ramström, Margareta; Hagman, Charlotte; Tsybin, Youri O; Markides, Karin E; Håkansson, Per; Salehi, S Albert LU ; Lundquist, Ingmar LU ; Håkanson, Rolf LU and Bergquist, Jonas (2003) In European Journal of Biochemistry 270(15). p.3146-3152
Abstract
Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their... (More)
Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Biochemistry
volume
270
issue
15
pages
3146 - 3152
publisher
Wiley-Blackwell
external identifiers
  • wos:000184267300004
  • pmid:12869189
  • scopus:0042268140
ISSN
0014-2956
DOI
10.1046/j.1432-1033.2003.03690.x
language
English
LU publication?
yes
id
b845f0e7-020b-4f09-8730-3555d2e7c9bf (old id 116377)
date added to LUP
2007-07-19 08:46:20
date last changed
2017-03-12 04:15:17
@article{b845f0e7-020b-4f09-8730-3555d2e7c9bf,
  abstract     = {Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts.},
  author       = {Ramström, Margareta and Hagman, Charlotte and Tsybin, Youri O and Markides, Karin E and Håkansson, Per and Salehi, S Albert and Lundquist, Ingmar and Håkanson, Rolf and Bergquist, Jonas},
  issn         = {0014-2956},
  language     = {eng},
  number       = {15},
  pages        = {3146--3152},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets.},
  url          = {http://dx.doi.org/10.1046/j.1432-1033.2003.03690.x},
  volume       = {270},
  year         = {2003},
}