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Verapamil Inhibits L-type Calcium Channel Mediated Apoptosis in Human Colon Cancer Cells.

Zawadzki, Antoni LU ; Liu, Qing LU ; Wang, Yusheng LU ; Melander, Arne LU ; Jeppsson, Bengt LU and Thorlacius, Henrik LU (2008) In Diseases of the Colon & Rectum 51. p.1696-1702
Abstract
PURPOSE: Treatment with calcium channel blockers have been associated with increased colon cancer mortality in epidemiologic studies. We examined the potential expression and function of calcium channels in two human colon cancer cell lines. METHODS: Both primary (collected at operation) and commercially-available human colon cancer cell lines were used. The colon cancer cells were incubated with a calcium channel blocker (verapamil) and a calcium channel agonist (BayK 8644) at clinically relevant concentrations. L-type calcium channel mRNA was determined by reverse-transcription polymerase chain reaction. Intracellular calcium ion levels were measured with fluorometry and apoptosis with flow cytometry. RESULTS: Both types of cells... (More)
PURPOSE: Treatment with calcium channel blockers have been associated with increased colon cancer mortality in epidemiologic studies. We examined the potential expression and function of calcium channels in two human colon cancer cell lines. METHODS: Both primary (collected at operation) and commercially-available human colon cancer cell lines were used. The colon cancer cells were incubated with a calcium channel blocker (verapamil) and a calcium channel agonist (BayK 8644) at clinically relevant concentrations. L-type calcium channel mRNA was determined by reverse-transcription polymerase chain reaction. Intracellular calcium ion levels were measured with fluorometry and apoptosis with flow cytometry. RESULTS: Both types of cells expressed L-type calcium channel mRNA, comprising an alpha-1D and a beta-3 subunit, whereas the cells were negative for N-type and P-type channels. The selective calcium channel agonist (BayK 8644), dose-dependently increased intracellular calcium ion levels and the level of apoptosis in primary human colon cancer cells. Pretreatment with verapamil completely abolished both calcium channel agonist-induced influx of calcium and apoptosis in these cells. CONCLUSIONS: These data demonstrate that human colon cancer cells express L-type calcium channels that mediate calcium influx and apoptosis, which warrants further studies to determine whether calcium channel blockers may promote colon cancer growth. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diseases of the Colon & Rectum
volume
51
pages
1696 - 1702
publisher
Springer
external identifiers
  • wos:000260371500019
  • pmid:18575938
  • scopus:54949157133
ISSN
0012-3706
DOI
10.1007/s10350-008-9372-7
language
English
LU publication?
yes
id
cabdbcbb-5165-48fd-bde6-0bb3ef875c99 (old id 1168475)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18575938?dopt=Abstract
date added to LUP
2008-07-02 13:19:41
date last changed
2017-07-30 04:49:28
@article{cabdbcbb-5165-48fd-bde6-0bb3ef875c99,
  abstract     = {PURPOSE: Treatment with calcium channel blockers have been associated with increased colon cancer mortality in epidemiologic studies. We examined the potential expression and function of calcium channels in two human colon cancer cell lines. METHODS: Both primary (collected at operation) and commercially-available human colon cancer cell lines were used. The colon cancer cells were incubated with a calcium channel blocker (verapamil) and a calcium channel agonist (BayK 8644) at clinically relevant concentrations. L-type calcium channel mRNA was determined by reverse-transcription polymerase chain reaction. Intracellular calcium ion levels were measured with fluorometry and apoptosis with flow cytometry. RESULTS: Both types of cells expressed L-type calcium channel mRNA, comprising an alpha-1D and a beta-3 subunit, whereas the cells were negative for N-type and P-type channels. The selective calcium channel agonist (BayK 8644), dose-dependently increased intracellular calcium ion levels and the level of apoptosis in primary human colon cancer cells. Pretreatment with verapamil completely abolished both calcium channel agonist-induced influx of calcium and apoptosis in these cells. CONCLUSIONS: These data demonstrate that human colon cancer cells express L-type calcium channels that mediate calcium influx and apoptosis, which warrants further studies to determine whether calcium channel blockers may promote colon cancer growth.},
  author       = {Zawadzki, Antoni and Liu, Qing and Wang, Yusheng and Melander, Arne and Jeppsson, Bengt and Thorlacius, Henrik},
  issn         = {0012-3706},
  language     = {eng},
  pages        = {1696--1702},
  publisher    = {Springer},
  series       = {Diseases of the Colon & Rectum},
  title        = {Verapamil Inhibits L-type Calcium Channel Mediated Apoptosis in Human Colon Cancer Cells.},
  url          = {http://dx.doi.org/10.1007/s10350-008-9372-7},
  volume       = {51},
  year         = {2008},
}