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LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells.

Jönsson, Daniel LU ; Nebel, Daniel LU ; Bratthall, Gunilla and Nilsson, Bengt-Olof LU (2008) In Archives of Oral Biology Jun 11. p.896-902
Abstract
OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptors but the functional importance of oestrogen in PDL cells exposed to bacterial endotoxins is not known. Here we investigate if the inflammation promoter lipopolysaccharide (LPS) affects PDL cell production of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), C-reactive protein (CRP) and/or normal functional PDL cell characteristics such as collagen synthesis and cell proliferation and if oestrogen modulates the effects of LPS. METHODS: PDL cells were obtained from periodontal ligament of premolars. PDL cells were treated with Escherichia coli LPS in the absence or presence of oestrogen (17beta-oestradiol, E(2)). Cellular concentration of IL-6, MCP-1 and... (More)
OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptors but the functional importance of oestrogen in PDL cells exposed to bacterial endotoxins is not known. Here we investigate if the inflammation promoter lipopolysaccharide (LPS) affects PDL cell production of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), C-reactive protein (CRP) and/or normal functional PDL cell characteristics such as collagen synthesis and cell proliferation and if oestrogen modulates the effects of LPS. METHODS: PDL cells were obtained from periodontal ligament of premolars. PDL cells were treated with Escherichia coli LPS in the absence or presence of oestrogen (17beta-oestradiol, E(2)). Cellular concentration of IL-6, MCP-1 and CRP was determined by enzyme-linked immunosorbent assay (ELISA). Collagen synthesis was determined by l-[(3)H]proline incorporation. Cell proliferation was assessed by DNA synthesis measurement using [(3)H]thymidine incorporation. RESULTS: Stimulation with LPS (500ng/ml to 10mug/ml) increased IL-6 production in a concentration-dependent manner. Lower concentration (100ng/ml) of LPS had no effect. LPS-induced stimulation of IL-6 was not reversed by a physiologically high concentration (100nM) of E(2). LPS increased also MCP-1 production which was unaffected by E(2). Treatment with E(2) alone had no effect on either IL-6 or MCP-1. Stimulation with LPS had no effect on CRP. LPS did not affect collagen synthesis and cell proliferation, reflecting normal physiological properties of PDL cells. CONCLUSIONS: LPS stimulates PDL cell IL-6 and MCP-1 production but has no effect on the normal physiological properties of PDL cells. LPS-induced IL-6 and MCP-1 is not reversed by oestrogen suggesting that oestrogen exerts no anti-inflammatory effect via this mechanism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Oral Biology
volume
Jun 11
pages
896 - 902
publisher
Elsevier
external identifiers
  • wos:000258474100013
  • pmid:18554572
  • scopus:47249116915
ISSN
1879-1506
DOI
10.1016/j.archoralbio.2008.05.001
language
English
LU publication?
yes
id
61d36364-0b11-4c96-b081-f087b8f035de (old id 1168802)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18554572?dopt=Abstract
date added to LUP
2008-07-03 12:55:56
date last changed
2017-08-13 04:37:03
@article{61d36364-0b11-4c96-b081-f087b8f035de,
  abstract     = {OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptors but the functional importance of oestrogen in PDL cells exposed to bacterial endotoxins is not known. Here we investigate if the inflammation promoter lipopolysaccharide (LPS) affects PDL cell production of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), C-reactive protein (CRP) and/or normal functional PDL cell characteristics such as collagen synthesis and cell proliferation and if oestrogen modulates the effects of LPS. METHODS: PDL cells were obtained from periodontal ligament of premolars. PDL cells were treated with Escherichia coli LPS in the absence or presence of oestrogen (17beta-oestradiol, E(2)). Cellular concentration of IL-6, MCP-1 and CRP was determined by enzyme-linked immunosorbent assay (ELISA). Collagen synthesis was determined by l-[(3)H]proline incorporation. Cell proliferation was assessed by DNA synthesis measurement using [(3)H]thymidine incorporation. RESULTS: Stimulation with LPS (500ng/ml to 10mug/ml) increased IL-6 production in a concentration-dependent manner. Lower concentration (100ng/ml) of LPS had no effect. LPS-induced stimulation of IL-6 was not reversed by a physiologically high concentration (100nM) of E(2). LPS increased also MCP-1 production which was unaffected by E(2). Treatment with E(2) alone had no effect on either IL-6 or MCP-1. Stimulation with LPS had no effect on CRP. LPS did not affect collagen synthesis and cell proliferation, reflecting normal physiological properties of PDL cells. CONCLUSIONS: LPS stimulates PDL cell IL-6 and MCP-1 production but has no effect on the normal physiological properties of PDL cells. LPS-induced IL-6 and MCP-1 is not reversed by oestrogen suggesting that oestrogen exerts no anti-inflammatory effect via this mechanism.},
  author       = {Jönsson, Daniel and Nebel, Daniel and Bratthall, Gunilla and Nilsson, Bengt-Olof},
  issn         = {1879-1506},
  language     = {eng},
  pages        = {896--902},
  publisher    = {Elsevier},
  series       = {Archives of Oral Biology},
  title        = {LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells.},
  url          = {http://dx.doi.org/10.1016/j.archoralbio.2008.05.001},
  volume       = {Jun 11},
  year         = {2008},
}