Polyamine Depletion with Two Different Polyamine Analogues Causes DNA Damage in Human Breast Cancer Cell Lines.
(2008) In DNA and Cell Biology 27(9). p.511-516- Abstract
- It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-depleted cells. In the present study we have used the comet assay to investigate polyamine depletion-induced DNA strand breaks. Three breast cancer cell lines and one normal-like breast cell line were treated with the polyamine analogue N(1),N(11)-diethylnorspermine or with the polyamine biosynthesis inhibitor 4-amidinoindan-1-one 2'-amidinohydrazone (CGP 48664). The comet assay showed that polyamine depletion resulted in DNA... (More)
- It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-depleted cells. In the present study we have used the comet assay to investigate polyamine depletion-induced DNA strand breaks. Three breast cancer cell lines and one normal-like breast cell line were treated with the polyamine analogue N(1),N(11)-diethylnorspermine or with the polyamine biosynthesis inhibitor 4-amidinoindan-1-one 2'-amidinohydrazone (CGP 48664). The comet assay showed that polyamine depletion resulted in DNA strand breaks. We also show that these DNA strand breaks occurred in cells where there was no expression of gamma-H2AX, which is a marker of DNA double-strand breaks. Thus, our conclusion is that polyamine depletion causes DNA single-strand breaks, which may be the cause for the observed delay in S phase progression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1168809
- author
- Johansson, Veronica LU ; Oredsson, Stina LU and Alm, Kersti LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- DNA and Cell Biology
- volume
- 27
- issue
- 9
- pages
- 511 - 516
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- wos:000259126800007
- scopus:50849135141
- pmid:18554080
- ISSN
- 1044-5498
- DOI
- 10.1089/dna.2008.0750
- language
- English
- LU publication?
- yes
- id
- d2cf5e69-7f94-402c-a10b-ddd5e2443648 (old id 1168809)
- date added to LUP
- 2016-04-01 13:19:23
- date last changed
- 2024-07-01 11:25:17
@article{d2cf5e69-7f94-402c-a10b-ddd5e2443648, abstract = {{It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-depleted cells. In the present study we have used the comet assay to investigate polyamine depletion-induced DNA strand breaks. Three breast cancer cell lines and one normal-like breast cell line were treated with the polyamine analogue N(1),N(11)-diethylnorspermine or with the polyamine biosynthesis inhibitor 4-amidinoindan-1-one 2'-amidinohydrazone (CGP 48664). The comet assay showed that polyamine depletion resulted in DNA strand breaks. We also show that these DNA strand breaks occurred in cells where there was no expression of gamma-H2AX, which is a marker of DNA double-strand breaks. Thus, our conclusion is that polyamine depletion causes DNA single-strand breaks, which may be the cause for the observed delay in S phase progression.}}, author = {{Johansson, Veronica and Oredsson, Stina and Alm, Kersti}}, issn = {{1044-5498}}, language = {{eng}}, number = {{9}}, pages = {{511--516}}, publisher = {{Mary Ann Liebert, Inc.}}, series = {{DNA and Cell Biology}}, title = {{Polyamine Depletion with Two Different Polyamine Analogues Causes DNA Damage in Human Breast Cancer Cell Lines.}}, url = {{http://dx.doi.org/10.1089/dna.2008.0750}}, doi = {{10.1089/dna.2008.0750}}, volume = {{27}}, year = {{2008}}, }