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Reprogramming of neonatal SVZ progenitors by Islet-1 and Neurogenin-2.

Rogelius, Nina LU ; Hebsgaard, Josephine B; Lundberg, Cecilia LU and Parmar, Malin LU (2008) In Molecular and Cellular Neuroscience 38(3). p.453-459
Abstract
The subventricular zone (SVZ) lining the lateral walls of the lateral ventricles is one of the major neurogenic areas in the postnatal brain. Precursor cells in the SVZ migrate via the rostral migratory stream to the olfactory bulb where they differentiate into neurons. Cell replacement strategies utilizing the recruitment of these endogenous progenitors and their progeny to different areas of the brain hold great promise for the future, but much research is needed in order to understand the sequence of molecular signals necessary to induce proliferation, migration and site-specific differentiation of these cells. In this study we show that the SVZ cells can be redirected from their normal migration route and directed towards other brain... (More)
The subventricular zone (SVZ) lining the lateral walls of the lateral ventricles is one of the major neurogenic areas in the postnatal brain. Precursor cells in the SVZ migrate via the rostral migratory stream to the olfactory bulb where they differentiate into neurons. Cell replacement strategies utilizing the recruitment of these endogenous progenitors and their progeny to different areas of the brain hold great promise for the future, but much research is needed in order to understand the sequence of molecular signals necessary to induce proliferation, migration and site-specific differentiation of these cells. In this study we show that the SVZ cells can be redirected from their normal migration route and directed towards other brain regions when they are infected with retroviruses encoding the developmentally important transcription factors Islet-1 and Neurogenin-2. After co-transduction with these transcription factors, transduced cells could be detected in several areas of the brain. When located in the striatum, the reprogrammed cells displayed neuroblast-like morphology. Once removed from the striatal parenchyma and allowed to further differentiation in vitro they developed into beta-III-tubulin positive neurons. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Neuroscience
volume
38
issue
3
pages
453 - 459
publisher
Elsevier
external identifiers
  • wos:000257553300014
  • pmid:18524626
  • scopus:45649085508
ISSN
1044-7431
DOI
10.1016/j.mcn.2008.04.008
language
English
LU publication?
yes
id
0de8be3c-6f91-4440-965c-5b64bf979847 (old id 1169183)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18524626?dopt=Abstract
date added to LUP
2008-07-02 11:44:42
date last changed
2017-01-01 07:30:16
@article{0de8be3c-6f91-4440-965c-5b64bf979847,
  abstract     = {The subventricular zone (SVZ) lining the lateral walls of the lateral ventricles is one of the major neurogenic areas in the postnatal brain. Precursor cells in the SVZ migrate via the rostral migratory stream to the olfactory bulb where they differentiate into neurons. Cell replacement strategies utilizing the recruitment of these endogenous progenitors and their progeny to different areas of the brain hold great promise for the future, but much research is needed in order to understand the sequence of molecular signals necessary to induce proliferation, migration and site-specific differentiation of these cells. In this study we show that the SVZ cells can be redirected from their normal migration route and directed towards other brain regions when they are infected with retroviruses encoding the developmentally important transcription factors Islet-1 and Neurogenin-2. After co-transduction with these transcription factors, transduced cells could be detected in several areas of the brain. When located in the striatum, the reprogrammed cells displayed neuroblast-like morphology. Once removed from the striatal parenchyma and allowed to further differentiation in vitro they developed into beta-III-tubulin positive neurons.},
  author       = {Rogelius, Nina and Hebsgaard, Josephine B and Lundberg, Cecilia and Parmar, Malin},
  issn         = {1044-7431},
  language     = {eng},
  number       = {3},
  pages        = {453--459},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Reprogramming of neonatal SVZ progenitors by Islet-1 and Neurogenin-2.},
  url          = {http://dx.doi.org/10.1016/j.mcn.2008.04.008},
  volume       = {38},
  year         = {2008},
}