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Inhibition of Rho kinase decreases hydraulic and protein microvascular permeability in cat skeletal muscle.

Lundblad, Cornelia LU ; Bentzer, Peter LU and Grände, Per-Olof LU (2003) In Microvascular Research 66(2). p.126-133
Abstract
Rho-associated kinases are involved in regulation of actin–myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influence microvascular permeability. The aim of the study was therefore to investigate whether Rho kinases influence hydraulic and protein microvascular permeability. The study was performed on the autoperfused cat skeletal muscle. A capillary filtration coefficient (CFC) technique was used to evaluate changes in hydraulic permeability, and protein permeability was evaluated by estimation of the change in the reflection coefficient... (More)
Rho-associated kinases are involved in regulation of actin–myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influence microvascular permeability. The aim of the study was therefore to investigate whether Rho kinases influence hydraulic and protein microvascular permeability. The study was performed on the autoperfused cat skeletal muscle. A capillary filtration coefficient (CFC) technique was used to evaluate changes in hydraulic permeability, and protein permeability was evaluated by estimation of the change in the reflection coefficient for albumin. In the first part of each experiment, the effects on CFC of three doses of the Rho kinase inhibitor Y-27632 of about 0.35, 0.70, and 1.05 μg/h per ml plasma flow were determined. There was a reduction in CFC at the lowest dose, and a tendency to further reduction at the higher doses used, reaching a decrease in CFC of 20%. The effects on CFC of the high and the middle dose did not differ. The reflection coefficient for albumin was increased by 31% following infusion of the highest dose of Y-27632. We conclude that hydraulic and protein microvascular permeability increase by Rho kinase activation, and that Rho kinase is involved in regulation of microvascular permeability. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Vascular permeability, Capillary filtration coefficient, Reflection coefficient, Proteins, Rho kinase, Rho kinase inhibition, Vascular resistance
in
Microvascular Research
volume
66
issue
2
pages
126 - 133
publisher
Academic Press
external identifiers
  • pmid:12935770
  • wos:000185050100006
  • scopus:0041620333
ISSN
1095-9319
DOI
language
English
LU publication?
yes
id
392de50d-8830-44db-88be-b730d63add1b (old id 117128)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12935770&dopt=Abstract
date added to LUP
2007-07-17 10:57:25
date last changed
2018-05-29 09:34:30
@article{392de50d-8830-44db-88be-b730d63add1b,
  abstract     = {Rho-associated kinases are involved in regulation of actin–myosin contractility and the organization of the actin cytoskeleton in both endothelial and smooth muscle cells. By influencing the contraction of the intraendothelial filaments, Rho kinases may affect the size of the interendothelial gaps and thereby influence microvascular permeability. The aim of the study was therefore to investigate whether Rho kinases influence hydraulic and protein microvascular permeability. The study was performed on the autoperfused cat skeletal muscle. A capillary filtration coefficient (CFC) technique was used to evaluate changes in hydraulic permeability, and protein permeability was evaluated by estimation of the change in the reflection coefficient for albumin. In the first part of each experiment, the effects on CFC of three doses of the Rho kinase inhibitor Y-27632 of about 0.35, 0.70, and 1.05 μg/h per ml plasma flow were determined. There was a reduction in CFC at the lowest dose, and a tendency to further reduction at the higher doses used, reaching a decrease in CFC of 20%. The effects on CFC of the high and the middle dose did not differ. The reflection coefficient for albumin was increased by 31% following infusion of the highest dose of Y-27632. We conclude that hydraulic and protein microvascular permeability increase by Rho kinase activation, and that Rho kinase is involved in regulation of microvascular permeability.},
  author       = {Lundblad, Cornelia and Bentzer, Peter and Grände, Per-Olof},
  issn         = {1095-9319},
  keyword      = {Vascular permeability,Capillary filtration coefficient,Reflection coefficient,Proteins,Rho kinase,Rho kinase inhibition,Vascular resistance},
  language     = {eng},
  number       = {2},
  pages        = {126--133},
  publisher    = {Academic Press},
  series       = {Microvascular Research},
  title        = {Inhibition of Rho kinase decreases hydraulic and protein microvascular permeability in cat skeletal muscle.},
  url          = {http://dx.doi.org/},
  volume       = {66},
  year         = {2003},
}