Advanced

Experimental Colonic Obstruction Increases Collagen Degradation by Matrix Metalloproteinases in the Bowel Wall.

Syk, Ingvar LU ; Mirastschijski, Ursula; Jeppsson, Bengt LU and Ågren, Magnus (2003) In Diseases of the Colon & Rectum 46(9). p.1251-1259
Abstract
PURPOSE: Emergency resections for colonic obstruction are accompanied with increased risk of anastomotic dehiscence. Elevated local degradation of submucosal collagens by matrix metalloproteinases may predispose to anastomotic leakage. This study was designed to study the effect of colon obstruction and surgical trauma on matrix metalloproteinase activities and correlate these results to collagen concentration in the colon wall. METHODS: Colonic obstruction was induced in male, Sprague-Dawley rats (n = 58) by applying a constricting silicone ring around the left colon 3 cm above the peritoneal reflection. After four days of obstruction, 2-mm wide colonic segments were resected approximately 3 mm proximal and 3 mm distal to the stenosis for... (More)
PURPOSE: Emergency resections for colonic obstruction are accompanied with increased risk of anastomotic dehiscence. Elevated local degradation of submucosal collagens by matrix metalloproteinases may predispose to anastomotic leakage. This study was designed to study the effect of colon obstruction and surgical trauma on matrix metalloproteinase activities and correlate these results to collagen concentration in the colon wall. METHODS: Colonic obstruction was induced in male, Sprague-Dawley rats (n = 58) by applying a constricting silicone ring around the left colon 3 cm above the peritoneal reflection. After four days of obstruction, 2-mm wide colonic segments were resected approximately 3 mm proximal and 3 mm distal to the stenosis for biochemical analyses. Colonic segments at corresponding locations were obtained from sham-operated rats (n = 5) without obstruction but with silicone ring placed adjacent to colon and from normal, nontraumatized rats (n = 10). Matrix metalloproteinase activity was determined by liberation of fragmented collagens from homogenized colonic tissue incubated ex vivo. Matrix metalloproteinase-2 specifically was analyzed by gelatin zymography. RESULTS: Endogenous collagenolysis by matrix metalloproteinases increased (P < 0.001) in colon as a consequence of obstruction (4.1-fold) and trauma (1.7-fold) compared with normal colon. In the proximity of the colon stenosis, total matrix metalloproteinase activity and matrix metalloproteinase-2 were significantly (P < 0.05) higher above than below the obstruction. Total activity was 22.9 (13.1–32.9) units/mg collagen proximal and 16.6 (12.7–18.4) units/mg collagen distal to the stenosis. Collagen concentration correlated inversely (r = –0.76; P < 0.001) with total matrix metalloproteinase activity. CONCLUSION: Colonic obstruction and trauma up-regulated matrix metalloproteinases and decreased collagen concentration in colonic wall. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colonic obstruction, Matrix metalloproteinase, Collagenase, Gelatinase, Intestinal healing
in
Diseases of the Colon & Rectum
volume
46
issue
9
pages
1251 - 1259
publisher
Springer
external identifiers
  • wos:000185384700014
  • pmid:12972971
  • scopus:0141675076
ISSN
0012-3706
DOI
language
English
LU publication?
yes
id
68b9f9eb-3f82-473d-abff-a25c08315763 (old id 117771)
date added to LUP
2007-07-30 11:02:06
date last changed
2018-05-29 11:10:04
@article{68b9f9eb-3f82-473d-abff-a25c08315763,
  abstract     = {PURPOSE: Emergency resections for colonic obstruction are accompanied with increased risk of anastomotic dehiscence. Elevated local degradation of submucosal collagens by matrix metalloproteinases may predispose to anastomotic leakage. This study was designed to study the effect of colon obstruction and surgical trauma on matrix metalloproteinase activities and correlate these results to collagen concentration in the colon wall. METHODS: Colonic obstruction was induced in male, Sprague-Dawley rats (n = 58) by applying a constricting silicone ring around the left colon 3 cm above the peritoneal reflection. After four days of obstruction, 2-mm wide colonic segments were resected approximately 3 mm proximal and 3 mm distal to the stenosis for biochemical analyses. Colonic segments at corresponding locations were obtained from sham-operated rats (n = 5) without obstruction but with silicone ring placed adjacent to colon and from normal, nontraumatized rats (n = 10). Matrix metalloproteinase activity was determined by liberation of fragmented collagens from homogenized colonic tissue incubated ex vivo. Matrix metalloproteinase-2 specifically was analyzed by gelatin zymography. RESULTS: Endogenous collagenolysis by matrix metalloproteinases increased (P &lt; 0.001) in colon as a consequence of obstruction (4.1-fold) and trauma (1.7-fold) compared with normal colon. In the proximity of the colon stenosis, total matrix metalloproteinase activity and matrix metalloproteinase-2 were significantly (P &lt; 0.05) higher above than below the obstruction. Total activity was 22.9 (13.1–32.9) units/mg collagen proximal and 16.6 (12.7–18.4) units/mg collagen distal to the stenosis. Collagen concentration correlated inversely (r = –0.76; P &lt; 0.001) with total matrix metalloproteinase activity. CONCLUSION: Colonic obstruction and trauma up-regulated matrix metalloproteinases and decreased collagen concentration in colonic wall.},
  author       = {Syk, Ingvar and Mirastschijski, Ursula and Jeppsson, Bengt and Ågren, Magnus},
  issn         = {0012-3706},
  keyword      = {Colonic obstruction,Matrix metalloproteinase,Collagenase,Gelatinase,Intestinal healing},
  language     = {eng},
  number       = {9},
  pages        = {1251--1259},
  publisher    = {Springer},
  series       = {Diseases of the Colon & Rectum},
  title        = {Experimental Colonic Obstruction Increases Collagen Degradation by Matrix Metalloproteinases in the Bowel Wall.},
  url          = {http://dx.doi.org/},
  volume       = {46},
  year         = {2003},
}