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Monitoring of Saccharomyces cerevisiae Cell Proliferation on Thiol-Modified Planar Gold Microelectrodes Using Impedance Spectroscopy.

Heiskanen, Arto LU ; Spegel, Christer LU ; Kostesha, Natalie LU ; Ruzgas, Tautgirdas LU and Emnéus, Jenny LU (2008) In Langmuir 24(16). p.9066-9073
Abstract
An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process of [Fe(CN)6] (3-/4-) showed a linear relationship to the number of cells even beyond the monolayer coverage. R ct showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R ct showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated... (More)
An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process of [Fe(CN)6] (3-/4-) showed a linear relationship to the number of cells even beyond the monolayer coverage. R ct showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R ct showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated that real-time monitoring of S. cerevisiae proliferation, with frequency-normalized imaginary admittance (real capacitance) as the indicator, was possible using a miniaturized culture system, ECIS Cultureware, with integrated planar cysteamine-modified Au microelectrodes. A monolayer coverage was reached after 20-28 h of cultivation, observed as an approximately 15% decrease in the real capacitance of the system. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
24
issue
16
pages
9066 - 9073
publisher
The American Chemical Society
external identifiers
  • wos:000258377900102
  • pmid:18630975
  • scopus:50849092849
ISSN
0743-7463
DOI
10.1021/la800580f
language
English
LU publication?
yes
id
11851008-91ef-4026-985b-20ce3d063050 (old id 1181102)
date added to LUP
2008-10-16 16:10:37
date last changed
2017-08-27 04:16:44
@article{11851008-91ef-4026-985b-20ce3d063050,
  abstract     = {An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process of [Fe(CN)6] (3-/4-) showed a linear relationship to the number of cells even beyond the monolayer coverage. R ct showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R ct showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated that real-time monitoring of S. cerevisiae proliferation, with frequency-normalized imaginary admittance (real capacitance) as the indicator, was possible using a miniaturized culture system, ECIS Cultureware, with integrated planar cysteamine-modified Au microelectrodes. A monolayer coverage was reached after 20-28 h of cultivation, observed as an approximately 15% decrease in the real capacitance of the system.},
  author       = {Heiskanen, Arto and Spegel, Christer and Kostesha, Natalie and Ruzgas, Tautgirdas and Emnéus, Jenny},
  issn         = {0743-7463},
  language     = {eng},
  number       = {16},
  pages        = {9066--9073},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Monitoring of Saccharomyces cerevisiae Cell Proliferation on Thiol-Modified Planar Gold Microelectrodes Using Impedance Spectroscopy.},
  url          = {http://dx.doi.org/10.1021/la800580f},
  volume       = {24},
  year         = {2008},
}