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Differentiated S100A7 expression in infected tonsils and tonsils from allergic individuals.

Bryborn, Malin LU ; Månsson, Anne; Cardell, Lars-Olaf LU and Adner, Mikael LU (2008) In Pathogens and Disease 53. p.413-420
Abstract
Palatine tonsils are continuously exposed to microorganisms and antigens and secrete antimicrobial peptides as a first line of defense. S100A7 is a protein with antimicrobial and chemotactic properties. Our aim was to investigate how the expression of S100A7 in human palatine tonsils is affected by inflammatory processes. Tonsils obtained from 109 patients undergoing tonsillectomy were divided into groups of infected and noninfected as well as allergic and nonallergic, based on the results from tonsillar core culture tests and Phadiatop analysis, respectively. Western blot and immunohistochemistry were used to assess protein expression and real-time PCR was used to quantify mRNA levels. To explore the induction of S100A7, tonsils were... (More)
Palatine tonsils are continuously exposed to microorganisms and antigens and secrete antimicrobial peptides as a first line of defense. S100A7 is a protein with antimicrobial and chemotactic properties. Our aim was to investigate how the expression of S100A7 in human palatine tonsils is affected by inflammatory processes. Tonsils obtained from 109 patients undergoing tonsillectomy were divided into groups of infected and noninfected as well as allergic and nonallergic, based on the results from tonsillar core culture tests and Phadiatop analysis, respectively. Western blot and immunohistochemistry were used to assess protein expression and real-time PCR was used to quantify mRNA levels. To explore the induction of S100A7, tonsils were stimulated with lipopolysaccharide in vitro. The immunohistochemical staining for S100A7 was most intense in the tonsillar epithelium, but the protein was also detected in B- and T-cell regions, which was confirmed with Western blot on isolated B and T cells. The S100A7 expression appeared to be the highest in CD8(+) T cells. Reduced mRNA levels of S100A7 were detected in infected tonsils as well as in tonsils from allergic individuals. In vitro stimulation of tonsils with lipopolysaccharide did not have any effect on the expression. The results suggest a role for S100A7 in recurrent tonsillitis and allergic disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pathogens and Disease
volume
53
pages
413 - 420
publisher
Wiley-Blackwell
external identifiers
  • wos:000258072600015
  • pmid:18625016
  • scopus:48449106952
ISSN
2049-632X
DOI
10.1111/j.1574-695X.2008.00444.x
language
English
LU publication?
yes
id
6e9ac2ee-ee70-4ed8-8c98-8395e01041b1 (old id 1181175)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18625016?dopt=Abstract
date added to LUP
2008-08-08 15:59:50
date last changed
2017-06-04 04:30:38
@article{6e9ac2ee-ee70-4ed8-8c98-8395e01041b1,
  abstract     = {Palatine tonsils are continuously exposed to microorganisms and antigens and secrete antimicrobial peptides as a first line of defense. S100A7 is a protein with antimicrobial and chemotactic properties. Our aim was to investigate how the expression of S100A7 in human palatine tonsils is affected by inflammatory processes. Tonsils obtained from 109 patients undergoing tonsillectomy were divided into groups of infected and noninfected as well as allergic and nonallergic, based on the results from tonsillar core culture tests and Phadiatop analysis, respectively. Western blot and immunohistochemistry were used to assess protein expression and real-time PCR was used to quantify mRNA levels. To explore the induction of S100A7, tonsils were stimulated with lipopolysaccharide in vitro. The immunohistochemical staining for S100A7 was most intense in the tonsillar epithelium, but the protein was also detected in B- and T-cell regions, which was confirmed with Western blot on isolated B and T cells. The S100A7 expression appeared to be the highest in CD8(+) T cells. Reduced mRNA levels of S100A7 were detected in infected tonsils as well as in tonsils from allergic individuals. In vitro stimulation of tonsils with lipopolysaccharide did not have any effect on the expression. The results suggest a role for S100A7 in recurrent tonsillitis and allergic disease.},
  author       = {Bryborn, Malin and Månsson, Anne and Cardell, Lars-Olaf and Adner, Mikael},
  issn         = {2049-632X},
  language     = {eng},
  pages        = {413--420},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {Differentiated S100A7 expression in infected tonsils and tonsils from allergic individuals.},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.2008.00444.x},
  volume       = {53},
  year         = {2008},
}