Disturbed alpha-cell function in mice with beta-cell specific overexpression of human islet amyloid polypeptide.
(2008) In Experimental Diabetes Research 2008.- Abstract
- Exogenous administration of islet amyloid polypeptide (IAPP) has been shown to inhibit both insulin and glucagon secretion. This study examined alpha-cell function in mice with beta-cell specific overexpression of human IAPP (hIAPP) after an oral protein gavage (75 mg whey protein/mouse). Baseline glucagon levels were higher in transgenic mice (41 +/- 4.0 pg/mL, n = 6) than in wildtype animals (19 +/- 5.1 pg/mL, n = 5, P = .015). In contrast, the glucagon response to protein was impaired in transgenic animals (21 +/- 2.7 pg/mL in transgenic mice versus 38 +/- 5.7 pg/mL in wildtype mice at 15 minutes; P = .027). Baseline insulin levels did not differ between the groups, while the insulin response, as the glucagon response, was impaired... (More)
- Exogenous administration of islet amyloid polypeptide (IAPP) has been shown to inhibit both insulin and glucagon secretion. This study examined alpha-cell function in mice with beta-cell specific overexpression of human IAPP (hIAPP) after an oral protein gavage (75 mg whey protein/mouse). Baseline glucagon levels were higher in transgenic mice (41 +/- 4.0 pg/mL, n = 6) than in wildtype animals (19 +/- 5.1 pg/mL, n = 5, P = .015). In contrast, the glucagon response to protein was impaired in transgenic animals (21 +/- 2.7 pg/mL in transgenic mice versus 38 +/- 5.7 pg/mL in wildtype mice at 15 minutes; P = .027). Baseline insulin levels did not differ between the groups, while the insulin response, as the glucagon response, was impaired after protein challenge (P = .018). Glucose levels were not different between the groups and did not change significantly after protein gavage. Acetaminophen was given through gavage to the animals (2 mg/mouse) to estimate gastric emptying. The plasma acetaminophen profile was similar in the two groups of mice. We conclude that disturbances in glucagon secretion exist in mice with beta-cell specific overexpression of human IAPP, which are not secondary to changes in gastric emptying. The reduced glucagon response to protein challenge may reflect a direct inhibitory influence of hIAPP on glucagon secretion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1181328
- author
- Ahrén, Bo LU and Sörhede Winzell, Maria LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Experimental Diabetes Research
- volume
- 2008
- article number
- 304513
- publisher
- Hindawi Limited
- external identifiers
-
- wos:000267260500001
- pmid:18615201
- scopus:52149087748
- ISSN
- 1687-5214
- DOI
- 10.1155/2008/304513
- language
- English
- LU publication?
- yes
- id
- 1c59e750-9ae9-443a-be15-06c4a700016a (old id 1181328)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18615201?dopt=Abstract
- date added to LUP
- 2016-04-04 08:33:17
- date last changed
- 2024-01-12 05:11:53
@article{1c59e750-9ae9-443a-be15-06c4a700016a,
abstract = {{Exogenous administration of islet amyloid polypeptide (IAPP) has been shown to inhibit both insulin and glucagon secretion. This study examined alpha-cell function in mice with beta-cell specific overexpression of human IAPP (hIAPP) after an oral protein gavage (75 mg whey protein/mouse). Baseline glucagon levels were higher in transgenic mice (41 +/- 4.0 pg/mL, n = 6) than in wildtype animals (19 +/- 5.1 pg/mL, n = 5, P = .015). In contrast, the glucagon response to protein was impaired in transgenic animals (21 +/- 2.7 pg/mL in transgenic mice versus 38 +/- 5.7 pg/mL in wildtype mice at 15 minutes; P = .027). Baseline insulin levels did not differ between the groups, while the insulin response, as the glucagon response, was impaired after protein challenge (P = .018). Glucose levels were not different between the groups and did not change significantly after protein gavage. Acetaminophen was given through gavage to the animals (2 mg/mouse) to estimate gastric emptying. The plasma acetaminophen profile was similar in the two groups of mice. We conclude that disturbances in glucagon secretion exist in mice with beta-cell specific overexpression of human IAPP, which are not secondary to changes in gastric emptying. The reduced glucagon response to protein challenge may reflect a direct inhibitory influence of hIAPP on glucagon secretion.}},
author = {{Ahrén, Bo and Sörhede Winzell, Maria}},
issn = {{1687-5214}},
language = {{eng}},
publisher = {{Hindawi Limited}},
series = {{Experimental Diabetes Research}},
title = {{Disturbed alpha-cell function in mice with beta-cell specific overexpression of human islet amyloid polypeptide.}},
url = {{http://dx.doi.org/10.1155/2008/304513}},
doi = {{10.1155/2008/304513}},
volume = {{2008}},
year = {{2008}},
}