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Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.

Andersson, Alexandra LU ; Edlund, Ulrica; Sjöberg, John; Albertsson, Ann-Christine and Stålbrand, Henrik LU (2008) In Biomacromolecules 9(8). p.2104-2110
Abstract
O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95%... (More)
O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
9
issue
8
pages
2104 - 2110
publisher
The American Chemical Society
external identifiers
  • wos:000258400200003
  • pmid:18590309
  • scopus:52649167335
ISSN
1526-4602
DOI
10.1021/bm701399m
language
English
LU publication?
yes
id
a7ef8330-b365-4fdf-ba81-896cb5a04f70 (old id 1181741)
date added to LUP
2008-10-03 12:55:34
date last changed
2017-07-23 03:54:08
@article{a7ef8330-b365-4fdf-ba81-896cb5a04f70,
  abstract     = {O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery.},
  author       = {Andersson, Alexandra and Edlund, Ulrica and Sjöberg, John and Albertsson, Ann-Christine and Stålbrand, Henrik},
  issn         = {1526-4602},
  language     = {eng},
  number       = {8},
  pages        = {2104--2110},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.},
  url          = {http://dx.doi.org/10.1021/bm701399m},
  volume       = {9},
  year         = {2008},
}