Trichoderma atroviride mutants with enhanced production of cellulase and beta-glucosidase on pretreated willow
(2008) In Enzyme and Microbial Technology 43(1). p.48-55- Abstract
- Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains... (More)
- Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains were measured in both shake flask and lab-scale fermenters and compared with Trichoderma reesei Rut C30. Also, hydrolytic capacities of fermentation supernatants of T reesei Rut C30, the parent strain (F-1505) and the best mutants were compared on pretreated willow as carbon source and hydrolysis substrate. The T atroviride mutants produced high levels of extracellular cellulases as well as beta-glucosidase, rendering the need for beta-glucosidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary. On the contrary, beta-glucosidase supplementations were essential in order to obtain good glucose yields for all other cellulase preparations tested. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1186973
- author
- Kovacs, Krisztina LU ; Megyeri, Laszlo ; Szakacs, George ; Kubicek, Christian P ; Galbe, Mats LU and Zacchi, Guido LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- enzymatic hydrolysis of, cellulose, fermentation, pretreated willow, production, beta-glucosidase, Trichoderma atroviride, cellulase production
- in
- Enzyme and Microbial Technology
- volume
- 43
- issue
- 1
- pages
- 48 - 55
- publisher
- Elsevier
- external identifiers
-
- wos:000257154400008
- scopus:44149103853
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2008.02.006
- language
- English
- LU publication?
- yes
- id
- 80810174-4c62-4e76-a782-65762bdcea85 (old id 1186973)
- date added to LUP
- 2016-04-01 11:50:50
- date last changed
- 2023-09-01 09:33:42
@article{80810174-4c62-4e76-a782-65762bdcea85,
abstract = {{Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains were measured in both shake flask and lab-scale fermenters and compared with Trichoderma reesei Rut C30. Also, hydrolytic capacities of fermentation supernatants of T reesei Rut C30, the parent strain (F-1505) and the best mutants were compared on pretreated willow as carbon source and hydrolysis substrate. The T atroviride mutants produced high levels of extracellular cellulases as well as beta-glucosidase, rendering the need for beta-glucosidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary. On the contrary, beta-glucosidase supplementations were essential in order to obtain good glucose yields for all other cellulase preparations tested.}},
author = {{Kovacs, Krisztina and Megyeri, Laszlo and Szakacs, George and Kubicek, Christian P and Galbe, Mats and Zacchi, Guido}},
issn = {{0141-0229}},
keywords = {{enzymatic hydrolysis of; cellulose; fermentation; pretreated willow; production; beta-glucosidase; Trichoderma atroviride; cellulase production}},
language = {{eng}},
number = {{1}},
pages = {{48--55}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Trichoderma atroviride mutants with enhanced production of cellulase and beta-glucosidase on pretreated willow}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2008.02.006}},
doi = {{10.1016/j.enzmictec.2008.02.006}},
volume = {{43}},
year = {{2008}},
}