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Trichoderma atroviride mutants with enhanced production of cellulase and beta-glucosidase on pretreated willow

Kovacs, Krisztina; Megyeri, Laszlo; Szakacs, George; Kubicek, Christian P; Galbe, Mats LU and Zacchi, Guido LU (2008) In Enzyme and Microbial Technology 43(1). p.48-55
Abstract
Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains... (More)
Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains were measured in both shake flask and lab-scale fermenters and compared with Trichoderma reesei Rut C30. Also, hydrolytic capacities of fermentation supernatants of T reesei Rut C30, the parent strain (F-1505) and the best mutants were compared on pretreated willow as carbon source and hydrolysis substrate. The T atroviride mutants produced high levels of extracellular cellulases as well as beta-glucosidase, rendering the need for beta-glucosidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary. On the contrary, beta-glucosidase supplementations were essential in order to obtain good glucose yields for all other cellulase preparations tested. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzymatic hydrolysis of, cellulose, fermentation, pretreated willow, production, beta-glucosidase, Trichoderma atroviride, cellulase production
in
Enzyme and Microbial Technology
volume
43
issue
1
pages
48 - 55
publisher
Elsevier
external identifiers
  • wos:000257154400008
  • scopus:44149103853
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2008.02.006
language
English
LU publication?
yes
id
80810174-4c62-4e76-a782-65762bdcea85 (old id 1186973)
date added to LUP
2008-09-04 10:27:15
date last changed
2017-09-24 03:33:14
@article{80810174-4c62-4e76-a782-65762bdcea85,
  abstract     = {Trichoderma atroviride mutants were developed by using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment and UV-Iight followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), beta-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains were measured in both shake flask and lab-scale fermenters and compared with Trichoderma reesei Rut C30. Also, hydrolytic capacities of fermentation supernatants of T reesei Rut C30, the parent strain (F-1505) and the best mutants were compared on pretreated willow as carbon source and hydrolysis substrate. The T atroviride mutants produced high levels of extracellular cellulases as well as beta-glucosidase, rendering the need for beta-glucosidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary. On the contrary, beta-glucosidase supplementations were essential in order to obtain good glucose yields for all other cellulase preparations tested.},
  author       = {Kovacs, Krisztina and Megyeri, Laszlo and Szakacs, George and Kubicek, Christian P and Galbe, Mats and Zacchi, Guido},
  issn         = {0141-0229},
  keyword      = {enzymatic hydrolysis of,cellulose,fermentation,pretreated willow,production,beta-glucosidase,Trichoderma atroviride,cellulase production},
  language     = {eng},
  number       = {1},
  pages        = {48--55},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Trichoderma atroviride mutants with enhanced production of cellulase and beta-glucosidase on pretreated willow},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2008.02.006},
  volume       = {43},
  year         = {2008},
}