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Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.

Jönsson, Daniel LU ; Andersson, G; Ekblad, Eva LU ; Liang, Min LU ; Bratthall, G and Nilsson, Bengt-Olof LU (2004) In Archives of Oral Biology 49(1). p.85-88
Abstract
Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Periodontal ligament cells, Estrogen receptors, Immunocytochemistry
in
Archives of Oral Biology
volume
49
issue
1
pages
85 - 88
publisher
Elsevier
external identifiers
  • wos:000188418200011
  • scopus:0842280811
ISSN
1879-1506
DOI
10.1016/S0003-9969(03)00198-5
language
English
LU publication?
yes
id
41c8c7a9-64a5-4984-97df-5f2ccff660ae (old id 119399)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14693201&dopt=Abstract
date added to LUP
2007-07-16 11:10:27
date last changed
2017-11-05 03:40:11
@article{41c8c7a9-64a5-4984-97df-5f2ccff660ae,
  abstract     = {Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.},
  author       = {Jönsson, Daniel and Andersson, G and Ekblad, Eva and Liang, Min and Bratthall, G and Nilsson, Bengt-Olof},
  issn         = {1879-1506},
  keyword      = {Periodontal ligament cells,Estrogen receptors,Immunocytochemistry},
  language     = {eng},
  number       = {1},
  pages        = {85--88},
  publisher    = {Elsevier},
  series       = {Archives of Oral Biology},
  title        = {Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.},
  url          = {http://dx.doi.org/10.1016/S0003-9969(03)00198-5},
  volume       = {49},
  year         = {2004},
}