Lower active force generation and improved fatigue resistance in skeletal muscle from desmin deficient mice.
(2003) In Journal of Muscle Research and Cell Motility 24(7). p.453-459- Abstract
- The mechanical effects of the intermediate filament protein desmin was examined in desmin deficient mice (Des-/-) and their wild type control (Des+/+). Active force generation was determined in intact soleus muscles and in skinned single fibres from soleus and psoas. A decreased force generation of skinned muscle fibres from Des-/- mice and a tendency towards decreased active force in intact soleus muscle were detected. Concentrations of the contractile protein actin and myosin were not altered in Des-/- muscles. Ca(2+)-sensitivity of skinned single fibres in Des-/- muscles was unchanged compared to Des+/+. Using a protocol with repeated short tetani an increased fatigue resistance was found in the intact soleus muscles from Des-/- mice.... (More)
- The mechanical effects of the intermediate filament protein desmin was examined in desmin deficient mice (Des-/-) and their wild type control (Des+/+). Active force generation was determined in intact soleus muscles and in skinned single fibres from soleus and psoas. A decreased force generation of skinned muscle fibres from Des-/- mice and a tendency towards decreased active force in intact soleus muscle were detected. Concentrations of the contractile protein actin and myosin were not altered in Des-/- muscles. Ca(2+)-sensitivity of skinned single fibres in Des-/- muscles was unchanged compared to Des+/+. Using a protocol with repeated short tetani an increased fatigue resistance was found in the intact soleus muscles from Des-/- mice. In conclusion, desmin intermediate filaments are required for optimal generation or transmission of active force in skeletal muscle. Although other studies have shown that the desmin intermediate filaments appear to influence Ca(2+)-handling, the Ca(2+)-sensitivity of the contractile filaments is not altered in skeletal muscle of Des-/- mice. Previous studies have reported a switch towards slower myosin isoforms in slow skeletal muscle of Des-/- mice. The increased fatigue resistance show that this change is reflected in the physiological function of the muscle. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/119601
- author
- Balogh, Johanna LU ; Li, Z ; Paulin, D and Arner, Anders LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Muscle Research and Cell Motility
- volume
- 24
- issue
- 7
- pages
- 453 - 459
- publisher
- Springer
- external identifiers
-
- wos:000186410900008
- pmid:14677648
- scopus:0344826002
- ISSN
- 0142-4319
- DOI
- 10.1023/A:1027353930229
- language
- English
- LU publication?
- yes
- id
- 16fddba6-8c2e-41fd-8561-f53f9e271045 (old id 119601)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14677648&dopt=Abstract
- date added to LUP
- 2016-04-01 16:55:33
- date last changed
- 2022-01-28 23:06:38
@article{16fddba6-8c2e-41fd-8561-f53f9e271045, abstract = {{The mechanical effects of the intermediate filament protein desmin was examined in desmin deficient mice (Des-/-) and their wild type control (Des+/+). Active force generation was determined in intact soleus muscles and in skinned single fibres from soleus and psoas. A decreased force generation of skinned muscle fibres from Des-/- mice and a tendency towards decreased active force in intact soleus muscle were detected. Concentrations of the contractile protein actin and myosin were not altered in Des-/- muscles. Ca(2+)-sensitivity of skinned single fibres in Des-/- muscles was unchanged compared to Des+/+. Using a protocol with repeated short tetani an increased fatigue resistance was found in the intact soleus muscles from Des-/- mice. In conclusion, desmin intermediate filaments are required for optimal generation or transmission of active force in skeletal muscle. Although other studies have shown that the desmin intermediate filaments appear to influence Ca(2+)-handling, the Ca(2+)-sensitivity of the contractile filaments is not altered in skeletal muscle of Des-/- mice. Previous studies have reported a switch towards slower myosin isoforms in slow skeletal muscle of Des-/- mice. The increased fatigue resistance show that this change is reflected in the physiological function of the muscle.}}, author = {{Balogh, Johanna and Li, Z and Paulin, D and Arner, Anders}}, issn = {{0142-4319}}, language = {{eng}}, number = {{7}}, pages = {{453--459}}, publisher = {{Springer}}, series = {{Journal of Muscle Research and Cell Motility}}, title = {{Lower active force generation and improved fatigue resistance in skeletal muscle from desmin deficient mice.}}, url = {{http://dx.doi.org/10.1023/A:1027353930229}}, doi = {{10.1023/A:1027353930229}}, volume = {{24}}, year = {{2003}}, }