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Identification and genetic fingerprinting of Brachyspira species

Fellström, Claes; Råsback, Therese; Johansson, Karl-Erik; Olofsson, Tobias LU and Aspan, Anna (2008) In Journal of Microbiological Methods 72(2). p.133-140
Abstract
Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All... (More)
Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All five PCR systems accurately identified their targets, except for the nox gene-based B. intermedia-specific system, by which it was not possible to identify one of the presumed B. intermedia isolates, and the other B. intermedia-specific system, based on the 23S rRNA gene, gave a positive reaction for one B. innocens isolate. In an extended study, 46 additional isolates and the original eight isolates with the phenotypes of B. hyodysenteriae or B. intermedia were compared by PFGE and PCR. The PFGE results indicated a high genetic diversity of isolates with the phenotype of B. intermedia. Thirty-three of 34 tested isolates could be identified by one or both of the two B. intennedia-specific PCR systems used, however, only 19 of the 34 isolates were positive in both systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
identification, Brachyspira, fingerprinting, typing
in
Journal of Microbiological Methods
volume
72
issue
2
pages
133 - 140
publisher
Elsevier
external identifiers
  • wos:000253214600004
  • scopus:38049105807
ISSN
1872-8359
DOI
10.1016/j.mimet.2007.11.015
language
English
LU publication?
yes
id
9d785dcb-a7bf-48c2-a5ff-8ffbaa39c2c4 (old id 1196473)
date added to LUP
2008-09-10 11:30:45
date last changed
2017-07-09 03:38:09
@article{9d785dcb-a7bf-48c2-a5ff-8ffbaa39c2c4,
  abstract     = {Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All five PCR systems accurately identified their targets, except for the nox gene-based B. intermedia-specific system, by which it was not possible to identify one of the presumed B. intermedia isolates, and the other B. intermedia-specific system, based on the 23S rRNA gene, gave a positive reaction for one B. innocens isolate. In an extended study, 46 additional isolates and the original eight isolates with the phenotypes of B. hyodysenteriae or B. intermedia were compared by PFGE and PCR. The PFGE results indicated a high genetic diversity of isolates with the phenotype of B. intermedia. Thirty-three of 34 tested isolates could be identified by one or both of the two B. intennedia-specific PCR systems used, however, only 19 of the 34 isolates were positive in both systems.},
  author       = {Fellström, Claes and Råsback, Therese and Johansson, Karl-Erik and Olofsson, Tobias and Aspan, Anna},
  issn         = {1872-8359},
  keyword      = {identification,Brachyspira,fingerprinting,typing},
  language     = {eng},
  number       = {2},
  pages        = {133--140},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Identification and genetic fingerprinting of Brachyspira species},
  url          = {http://dx.doi.org/10.1016/j.mimet.2007.11.015},
  volume       = {72},
  year         = {2008},
}