Identification and genetic fingerprinting of Brachyspira species
(2008) In Journal of Microbiological Methods 72(2). p.133-140- Abstract
- Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All... (More)
- Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All five PCR systems accurately identified their targets, except for the nox gene-based B. intermedia-specific system, by which it was not possible to identify one of the presumed B. intermedia isolates, and the other B. intermedia-specific system, based on the 23S rRNA gene, gave a positive reaction for one B. innocens isolate. In an extended study, 46 additional isolates and the original eight isolates with the phenotypes of B. hyodysenteriae or B. intermedia were compared by PFGE and PCR. The PFGE results indicated a high genetic diversity of isolates with the phenotype of B. intermedia. Thirty-three of 34 tested isolates could be identified by one or both of the two B. intennedia-specific PCR systems used, however, only 19 of the 34 isolates were positive in both systems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1196473
- author
- Fellström, Claes ; Råsback, Therese ; Johansson, Karl-Erik ; Olofsson, Tobias LU and Aspan, Anna
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- identification, Brachyspira, fingerprinting, typing
- in
- Journal of Microbiological Methods
- volume
- 72
- issue
- 2
- pages
- 133 - 140
- publisher
- Elsevier
- external identifiers
-
- wos:000253214600004
- scopus:38049105807
- ISSN
- 1872-8359
- DOI
- 10.1016/j.mimet.2007.11.015
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biology building (Closed 2011) (011008000)
- id
- 9d785dcb-a7bf-48c2-a5ff-8ffbaa39c2c4 (old id 1196473)
- date added to LUP
- 2016-04-01 12:02:26
- date last changed
- 2022-01-26 21:56:16
@article{9d785dcb-a7bf-48c2-a5ff-8ffbaa39c2c4,
abstract = {{Six Brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine Brachyspira spp. were compared by different molecular methods. Sequence analysis of the 16S rRNA and the nox genes, pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) were used in the study. In addition the isolates were analysed by five species-specific PCR systems. The topologies of the dendrograms obtained from each of the four typing systems were different. The B. pilosicoli isolates formed monophyletic clusters in all dendrograms, but with different sister lines. All five porcine Brachyspira species formed monophyletic clusters in the nox gene-based dendrogram only. All five PCR systems accurately identified their targets, except for the nox gene-based B. intermedia-specific system, by which it was not possible to identify one of the presumed B. intermedia isolates, and the other B. intermedia-specific system, based on the 23S rRNA gene, gave a positive reaction for one B. innocens isolate. In an extended study, 46 additional isolates and the original eight isolates with the phenotypes of B. hyodysenteriae or B. intermedia were compared by PFGE and PCR. The PFGE results indicated a high genetic diversity of isolates with the phenotype of B. intermedia. Thirty-three of 34 tested isolates could be identified by one or both of the two B. intennedia-specific PCR systems used, however, only 19 of the 34 isolates were positive in both systems.}},
author = {{Fellström, Claes and Råsback, Therese and Johansson, Karl-Erik and Olofsson, Tobias and Aspan, Anna}},
issn = {{1872-8359}},
keywords = {{identification; Brachyspira; fingerprinting; typing}},
language = {{eng}},
number = {{2}},
pages = {{133--140}},
publisher = {{Elsevier}},
series = {{Journal of Microbiological Methods}},
title = {{Identification and genetic fingerprinting of Brachyspira species}},
url = {{http://dx.doi.org/10.1016/j.mimet.2007.11.015}},
doi = {{10.1016/j.mimet.2007.11.015}},
volume = {{72}},
year = {{2008}},
}