Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054
(2008) In Biotechnology and Bioengineering 99(4). p.783-790- Abstract
- Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400... (More)
- Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400 consumed all glucose and large fraction of the xylose in SSF. Almost complete xylose conversion could be achieved at 5% WIS and 32 degrees C. Fermentation did not occur with P. stipitis CBS6054 at pH 5.0. However, at pH 6.0, complete glucose conversion and high xylose conversion (> 70%) was obtained. Microaeration was required for P. stipitis CBS6054. This was not necessary for S. cerevisiae TMB3400. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1196972
- author
- Rudolf, Andreas LU ; Baudel, Henrique ; Zacchi, Guido LU ; Hahn-Hägerdal, Bärbel LU and Lidén, Gunnar LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- strain comparison, Pichia stipitis CBS6054, Saccharomyces TMB3400, xylose fermentation, SSF, sugar cane bagasse
- in
- Biotechnology and Bioengineering
- volume
- 99
- issue
- 4
- pages
- 783 - 790
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000253277300005
- scopus:39549104535
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.21636
- language
- English
- LU publication?
- yes
- id
- dfc2e1ef-8e6a-45a8-b051-f377df50dec8 (old id 1196972)
- date added to LUP
- 2016-04-01 11:56:38
- date last changed
- 2023-11-11 06:51:46
@article{dfc2e1ef-8e6a-45a8-b051-f377df50dec8,
abstract = {{Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400 consumed all glucose and large fraction of the xylose in SSF. Almost complete xylose conversion could be achieved at 5% WIS and 32 degrees C. Fermentation did not occur with P. stipitis CBS6054 at pH 5.0. However, at pH 6.0, complete glucose conversion and high xylose conversion (> 70%) was obtained. Microaeration was required for P. stipitis CBS6054. This was not necessary for S. cerevisiae TMB3400.}},
author = {{Rudolf, Andreas and Baudel, Henrique and Zacchi, Guido and Hahn-Hägerdal, Bärbel and Lidén, Gunnar}},
issn = {{1097-0290}},
keywords = {{strain comparison; Pichia stipitis CBS6054; Saccharomyces TMB3400; xylose fermentation; SSF; sugar cane bagasse}},
language = {{eng}},
number = {{4}},
pages = {{783--790}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054}},
url = {{http://dx.doi.org/10.1002/bit.21636}},
doi = {{10.1002/bit.21636}},
volume = {{99}},
year = {{2008}},
}