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Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054

Rudolf, Andreas LU ; Baudel, Henrique; Zacchi, Guido LU ; Hahn-Hägerdal, Bärbel LU and Lidén, Gunnar LU (2008) In Biotechnology and Bioengineering 99(4). p.783-790
Abstract
Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400... (More)
Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400 consumed all glucose and large fraction of the xylose in SSF. Almost complete xylose conversion could be achieved at 5% WIS and 32 degrees C. Fermentation did not occur with P. stipitis CBS6054 at pH 5.0. However, at pH 6.0, complete glucose conversion and high xylose conversion (> 70%) was obtained. Microaeration was required for P. stipitis CBS6054. This was not necessary for S. cerevisiae TMB3400. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
strain comparison, Pichia stipitis CBS6054, Saccharomyces TMB3400, xylose fermentation, SSF, sugar cane bagasse
in
Biotechnology and Bioengineering
volume
99
issue
4
pages
783 - 790
publisher
John Wiley & Sons
external identifiers
  • wos:000253277300005
  • scopus:39549104535
ISSN
1097-0290
DOI
10.1002/bit.21636
language
English
LU publication?
yes
id
dfc2e1ef-8e6a-45a8-b051-f377df50dec8 (old id 1196972)
date added to LUP
2008-09-10 12:58:49
date last changed
2017-08-06 03:37:41
@article{dfc2e1ef-8e6a-45a8-b051-f377df50dec8,
  abstract     = {Sugarcane bagasse-a residue from sugar and ethanol production from sugar cane-is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol, In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using Saccharomyces cerevisiae TMB3400 a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400 consumed all glucose and large fraction of the xylose in SSF. Almost complete xylose conversion could be achieved at 5% WIS and 32 degrees C. Fermentation did not occur with P. stipitis CBS6054 at pH 5.0. However, at pH 6.0, complete glucose conversion and high xylose conversion (> 70%) was obtained. Microaeration was required for P. stipitis CBS6054. This was not necessary for S. cerevisiae TMB3400.},
  author       = {Rudolf, Andreas and Baudel, Henrique and Zacchi, Guido and Hahn-Hägerdal, Bärbel and Lidén, Gunnar},
  issn         = {1097-0290},
  keyword      = {strain comparison,Pichia stipitis CBS6054,Saccharomyces TMB3400,xylose fermentation,SSF,sugar cane bagasse},
  language     = {eng},
  number       = {4},
  pages        = {783--790},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054},
  url          = {http://dx.doi.org/10.1002/bit.21636},
  volume       = {99},
  year         = {2008},
}