Diffuse axonal damage, myelin impairment, astrocytosis and inflammatory response following microinjections of NMDA into the rat striatum
(2008) In Inflammation 31(1). p.24-35- Abstract
- White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred... (More)
- White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred at 1 and 7 days post-nmda injection, respectively. Diffuse damaged axons (beta-app + end-bulbs) were apparent at 7 days, concomitant with progressive myelin impairment and astrocytosis. Further studies using electron microscopy and blockers of inflammatory response and glutamatergic receptors should be performed to confirm and address the mechanisms of white matter damage following an excitotoxic lesion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1198684
- author
- Lima, Rafael R ; Guimaraes-Silva, Joanilson ; Oliveira, Jorge L ; Costa, Ana Maria R ; Souza-Rodrigues, Renata D ; Dos Santos, Claudia D ; Picanco-Diniz, Cristovam W and Gomes-Leal, Wallace LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- neutrophils, macrophages, excitotoxicity, white matter damage, basal ganglia
- in
- Inflammation
- volume
- 31
- issue
- 1
- pages
- 24 - 35
- publisher
- Springer
- external identifiers
-
- wos:000253007100003
- scopus:39049117404
- ISSN
- 0360-3997
- DOI
- 10.1007/s10753-007-9046-y
- language
- English
- LU publication?
- yes
- id
- 2ebeea3c-32dc-4489-9b93-18a60a6dcd76 (old id 1198684)
- date added to LUP
- 2016-04-01 12:12:28
- date last changed
- 2022-04-21 03:58:54
@article{2ebeea3c-32dc-4489-9b93-18a60a6dcd76,
abstract = {{White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred at 1 and 7 days post-nmda injection, respectively. Diffuse damaged axons (beta-app + end-bulbs) were apparent at 7 days, concomitant with progressive myelin impairment and astrocytosis. Further studies using electron microscopy and blockers of inflammatory response and glutamatergic receptors should be performed to confirm and address the mechanisms of white matter damage following an excitotoxic lesion.}},
author = {{Lima, Rafael R and Guimaraes-Silva, Joanilson and Oliveira, Jorge L and Costa, Ana Maria R and Souza-Rodrigues, Renata D and Dos Santos, Claudia D and Picanco-Diniz, Cristovam W and Gomes-Leal, Wallace}},
issn = {{0360-3997}},
keywords = {{neutrophils; macrophages; excitotoxicity; white matter damage; basal ganglia}},
language = {{eng}},
number = {{1}},
pages = {{24--35}},
publisher = {{Springer}},
series = {{Inflammation}},
title = {{Diffuse axonal damage, myelin impairment, astrocytosis and inflammatory response following microinjections of NMDA into the rat striatum}},
url = {{http://dx.doi.org/10.1007/s10753-007-9046-y}},
doi = {{10.1007/s10753-007-9046-y}},
volume = {{31}},
year = {{2008}},
}