Advanced

Diffuse axonal damage, myelin impairment, astrocytosis and inflammatory response following microinjections of NMDA into the rat striatum

Lima, Rafael R; Guimaraes-Silva, Joanilson; Oliveira, Jorge L; Costa, Ana Maria R; Souza-Rodrigues, Renata D; Dos Santos, Claudia D; Picanco-Diniz, Cristovam W and Gomes-Leal, Wallace LU (2008) In Inflammation 31(1). p.24-35
Abstract
White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred... (More)
White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred at 1 and 7 days post-nmda injection, respectively. Diffuse damaged axons (beta-app + end-bulbs) were apparent at 7 days, concomitant with progressive myelin impairment and astrocytosis. Further studies using electron microscopy and blockers of inflammatory response and glutamatergic receptors should be performed to confirm and address the mechanisms of white matter damage following an excitotoxic lesion. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neutrophils, macrophages, excitotoxicity, white matter damage, basal ganglia
in
Inflammation
volume
31
issue
1
pages
24 - 35
publisher
Springer
external identifiers
  • wos:000253007100003
  • scopus:39049117404
ISSN
0360-3997
DOI
10.1007/s10753-007-9046-y
language
English
LU publication?
yes
id
2ebeea3c-32dc-4489-9b93-18a60a6dcd76 (old id 1198684)
date added to LUP
2008-09-10 16:19:36
date last changed
2017-01-22 03:32:12
@article{2ebeea3c-32dc-4489-9b93-18a60a6dcd76,
  abstract     = {White matter damage and inflammatory response are important secondary outcomes after acute neural disorders. Nevertheless, a few studies addressed the temporal outcomes of these pathological events using non-traumatic models of acute brain injury. In the present study, we describe acute inflammatory response and white matter neuropathology between 1 and 7 days after acute excitotoxic striatal damage. Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils (anti-mbs-1), activated macrophages/microglia (anti-ed1), astrocytes (anti-gfap), damaged axons (anti-beta app) and myelin basic protein (MBP). Recruitment peak of neutrophils and macrophages occurred at 1 and 7 days post-nmda injection, respectively. Diffuse damaged axons (beta-app + end-bulbs) were apparent at 7 days, concomitant with progressive myelin impairment and astrocytosis. Further studies using electron microscopy and blockers of inflammatory response and glutamatergic receptors should be performed to confirm and address the mechanisms of white matter damage following an excitotoxic lesion.},
  author       = {Lima, Rafael R and Guimaraes-Silva, Joanilson and Oliveira, Jorge L and Costa, Ana Maria R and Souza-Rodrigues, Renata D and Dos Santos, Claudia D and Picanco-Diniz, Cristovam W and Gomes-Leal, Wallace},
  issn         = {0360-3997},
  keyword      = {neutrophils,macrophages,excitotoxicity,white matter damage,basal ganglia},
  language     = {eng},
  number       = {1},
  pages        = {24--35},
  publisher    = {Springer},
  series       = {Inflammation},
  title        = {Diffuse axonal damage, myelin impairment, astrocytosis and inflammatory response following microinjections of NMDA into the rat striatum},
  url          = {http://dx.doi.org/10.1007/s10753-007-9046-y},
  volume       = {31},
  year         = {2008},
}