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Cystatin C in the anterior segment of rat and mouse eyes.

Wasselius, Johan LU ; Håkansson, Katarina LU ; Abrahamson, Magnus LU and Ehinger, Berndt LU (2004) In Acta Ophthalmologica Scandinavica1998-01-01+01:002008-01-01+01:00 82(1). p.68-75
Abstract
Purpose: Cystatin C is a mammalian cysteine protease inhibitor. This study describes the localization of cystatin C in the anterior segment of normal rat and mouse eyes. Cysteine proteases play an important role in protein degradation (e.g. of photoreceptor outer segments in the retinal pigment epithelium) and the balance between these proteases and their specific inhibitors is therefore of great interest.



Methods: Cells containing cystatin C were identified by immunohistochemistry and quantified by ELISA. Messenger RNA levels were analysed by quantitative real-time polymerase chain reaction.



Results: Cystatin C is present at biologically significant levels in the corneal epithelium, endothelium and... (More)
Purpose: Cystatin C is a mammalian cysteine protease inhibitor. This study describes the localization of cystatin C in the anterior segment of normal rat and mouse eyes. Cysteine proteases play an important role in protein degradation (e.g. of photoreceptor outer segments in the retinal pigment epithelium) and the balance between these proteases and their specific inhibitors is therefore of great interest.



Methods: Cells containing cystatin C were identified by immunohistochemistry and quantified by ELISA. Messenger RNA levels were analysed by quantitative real-time polymerase chain reaction.



Results: Cystatin C is present at biologically significant levels in the corneal epithelium, endothelium and stromal keratinocytes, lens epithelium, epithelial cells in the ciliary processes, aqueous humour and iris stromal cells. In the rat anterior segment, the highest cystatin C concentrations were found in the ciliary epithelium.



Conclusions: Cystatin C is present in several cell types and is probably locally produced. The inhibitor is likely to be an important regulator of cysteine proteases in the retinal pigment epithelium, ciliary epithelium, aqueous humour, lens epithelium and in the corneal endothelium and epithelium. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Ophthalmologica Scandinavica1998-01-01+01:002008-01-01+01:00
volume
82
issue
1
pages
68 - 75
publisher
Wiley-Blackwell
external identifiers
  • wos:000188409800014
  • scopus:1242275336
ISSN
1395-3907
DOI
10.1046/j.1600-0420.2003.00178.x
language
English
LU publication?
yes
id
bf5ec83e-34de-4e7a-852d-487c5804c0cb (old id 120009)
date added to LUP
2007-07-06 10:15:49
date last changed
2017-01-01 07:27:54
@article{bf5ec83e-34de-4e7a-852d-487c5804c0cb,
  abstract     = {Purpose: Cystatin C is a mammalian cysteine protease inhibitor. This study describes the localization of cystatin C in the anterior segment of normal rat and mouse eyes. Cysteine proteases play an important role in protein degradation (e.g. of photoreceptor outer segments in the retinal pigment epithelium) and the balance between these proteases and their specific inhibitors is therefore of great interest.<br/><br>
<br/><br>
Methods: Cells containing cystatin C were identified by immunohistochemistry and quantified by ELISA. Messenger RNA levels were analysed by quantitative real-time polymerase chain reaction.<br/><br>
<br/><br>
Results: Cystatin C is present at biologically significant levels in the corneal epithelium, endothelium and stromal keratinocytes, lens epithelium, epithelial cells in the ciliary processes, aqueous humour and iris stromal cells. In the rat anterior segment, the highest cystatin C concentrations were found in the ciliary epithelium.<br/><br>
<br/><br>
Conclusions: Cystatin C is present in several cell types and is probably locally produced. The inhibitor is likely to be an important regulator of cysteine proteases in the retinal pigment epithelium, ciliary epithelium, aqueous humour, lens epithelium and in the corneal endothelium and epithelium.},
  author       = {Wasselius, Johan and Håkansson, Katarina and Abrahamson, Magnus and Ehinger, Berndt},
  issn         = {1395-3907},
  language     = {eng},
  number       = {1},
  pages        = {68--75},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Ophthalmologica Scandinavica1998-01-01+01:002008-01-01+01:00},
  title        = {Cystatin C in the anterior segment of rat and mouse eyes.},
  url          = {http://dx.doi.org/10.1046/j.1600-0420.2003.00178.x},
  volume       = {82},
  year         = {2004},
}