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Optical projection tomography for rapid whole mouse brain imaging

Nguyen, David; Marchand, Paul J.; Planchette, Arielle L.; Nilsson, Julia LU ; Sison, Miguel; Extermann, Jérôme; Lopez, Antonio; Sylwestrzak, Marcin; Sordet-Dessimoz, Jessica and Schmidt-Christensen, Anja LU , et al. (2017) In Biomedical Optics Express 8(12). p.5637-5650
Abstract

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.

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publication status
published
subject
in
Biomedical Optics Express
volume
8
issue
12
pages
14 pages
publisher
Optical Society of America
external identifiers
  • scopus:85037340369
ISSN
2156-7085
DOI
10.1364/BOE.8.005637
language
English
LU publication?
yes
id
1203305c-fc79-41bd-9a34-3222a4b198a4
date added to LUP
2017-12-21 12:15:54
date last changed
2018-01-07 12:29:35
@article{1203305c-fc79-41bd-9a34-3222a4b198a4,
  abstract     = {<p>In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.</p>},
  articleno    = {#301816},
  author       = {Nguyen, David and Marchand, Paul J. and Planchette, Arielle L. and Nilsson, Julia and Sison, Miguel and Extermann, Jérôme and Lopez, Antonio and Sylwestrzak, Marcin and Sordet-Dessimoz, Jessica and Schmidt-Christensen, Anja and Holmberg, Dan and Van De Ville, Dimitri and Lasser, Theo},
  issn         = {2156-7085},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {5637--5650},
  publisher    = {Optical Society of America},
  series       = {Biomedical Optics Express},
  title        = {Optical projection tomography for rapid whole mouse brain imaging},
  url          = {http://dx.doi.org/10.1364/BOE.8.005637},
  volume       = {8},
  year         = {2017},
}