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Direct heterogeneous electron transfer reactions of Bacillus halodurans bacterial blue multicopper oxidase

Shleev, Sergey LU ; Wang, Yan; Gorbacheva, Marina; Christenson, Andreas LU ; Haltrich, Dietmar; Ludwig, Roland; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2008) In Electroanalysis 20(9). p.963-969
Abstract
Direct electron transfer reactions of Bacillus halodurans bacterial multicopper oxidase on bare spectrographic graphite, as well as bare and thiol-modified gold electrodes were studied using cyclic voltammetry, potentiometry, amperometry, and spectroelectrochemistry. The redox potential of the T1 site of the enzyme was measured using mediatorless redox titration and found to be 325 mV +/- 10 mV vs. NHE. From measurements with a mercaptopropionic acid-modified gold electrode under aerobic conditions a midpoint potential of 360 mV vs. NHE for the T2/T3 copper cluster is deduced. Differing from most other characterized laccases of fungal and plant origins this bacterial enzyme exhibits bioelectrocatalytic activity at neutral pH and tolerates... (More)
Direct electron transfer reactions of Bacillus halodurans bacterial multicopper oxidase on bare spectrographic graphite, as well as bare and thiol-modified gold electrodes were studied using cyclic voltammetry, potentiometry, amperometry, and spectroelectrochemistry. The redox potential of the T1 site of the enzyme was measured using mediatorless redox titration and found to be 325 mV +/- 10 mV vs. NHE. From measurements with a mercaptopropionic acid-modified gold electrode under aerobic conditions a midpoint potential of 360 mV vs. NHE for the T2/T3 copper cluster is deduced. Differing from most other characterized laccases of fungal and plant origins this bacterial enzyme exhibits bioelectrocatalytic activity at neutral pH and tolerates high chloride concentrations (200 mM), conditions that usually strongly inhibit catalysis. Moreover, it has the very high affinity towards molecular oxygen both in solution and in the adsorbed state (K-M <= 50 mu M). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
T1 site, redox potential, bacterial multicopper oxidase, carbon, gold
in
Electroanalysis
volume
20
issue
9
pages
963 - 969
publisher
John Wiley & Sons
external identifiers
  • wos:000255770800005
  • scopus:54549122413
ISSN
1040-0397
DOI
10.1002/elan.200704116
language
English
LU publication?
yes
id
d265650e-c3f0-4272-8952-9648515be3c0 (old id 1204228)
date added to LUP
2008-09-17 12:27:54
date last changed
2017-01-01 06:04:54
@article{d265650e-c3f0-4272-8952-9648515be3c0,
  abstract     = {Direct electron transfer reactions of Bacillus halodurans bacterial multicopper oxidase on bare spectrographic graphite, as well as bare and thiol-modified gold electrodes were studied using cyclic voltammetry, potentiometry, amperometry, and spectroelectrochemistry. The redox potential of the T1 site of the enzyme was measured using mediatorless redox titration and found to be 325 mV +/- 10 mV vs. NHE. From measurements with a mercaptopropionic acid-modified gold electrode under aerobic conditions a midpoint potential of 360 mV vs. NHE for the T2/T3 copper cluster is deduced. Differing from most other characterized laccases of fungal and plant origins this bacterial enzyme exhibits bioelectrocatalytic activity at neutral pH and tolerates high chloride concentrations (200 mM), conditions that usually strongly inhibit catalysis. Moreover, it has the very high affinity towards molecular oxygen both in solution and in the adsorbed state (K-M &lt;= 50 mu M).},
  author       = {Shleev, Sergey and Wang, Yan and Gorbacheva, Marina and Christenson, Andreas and Haltrich, Dietmar and Ludwig, Roland and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {T1 site,redox potential,bacterial multicopper oxidase,carbon,gold},
  language     = {eng},
  number       = {9},
  pages        = {963--969},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Direct heterogeneous electron transfer reactions of Bacillus halodurans bacterial blue multicopper oxidase},
  url          = {http://dx.doi.org/10.1002/elan.200704116},
  volume       = {20},
  year         = {2008},
}