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Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266

Rybka, Jacek; Grycko, Pawel; da Cruz Francisco, José LU ; Gamian, Andrzej and Dey, Estera LU (2008) In Journal of Supercritical Fluids 45(1). p.51-56
Abstract
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used,... (More)
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vaccine, Salmonella, lipopolysaccharides (LPS), supercritical carbon dioxide (scCO(2))
in
Journal of Supercritical Fluids
volume
45
issue
1
pages
51 - 56
publisher
Elsevier
external identifiers
  • wos:000255297400006
  • scopus:40949108080
ISSN
0896-8446
DOI
10.1016/j.supflu.2007.11.011
language
English
LU publication?
yes
id
757e3560-2db8-4142-96b9-c08f8d4a15dd (old id 1205253)
date added to LUP
2008-09-18 13:55:28
date last changed
2017-01-01 05:00:20
@article{757e3560-2db8-4142-96b9-c08f8d4a15dd,
  abstract     = {The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.},
  author       = {Rybka, Jacek and Grycko, Pawel and da Cruz Francisco, José and Gamian, Andrzej and Dey, Estera},
  issn         = {0896-8446},
  keyword      = {vaccine,Salmonella,lipopolysaccharides (LPS),supercritical carbon dioxide (scCO(2))},
  language     = {eng},
  number       = {1},
  pages        = {51--56},
  publisher    = {Elsevier},
  series       = {Journal of Supercritical Fluids},
  title        = {Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266},
  url          = {http://dx.doi.org/10.1016/j.supflu.2007.11.011},
  volume       = {45},
  year         = {2008},
}