Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266
(2008) In Journal of Supercritical Fluids 45(1). p.51-56- Abstract
- The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used,... (More)
- The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1205253
- author
- Rybka, Jacek ; Grycko, Pawel ; da Cruz Francisco, José LU ; Gamian, Andrzej and Dey, Estera LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- vaccine, Salmonella, lipopolysaccharides (LPS), supercritical carbon dioxide (scCO(2))
- in
- Journal of Supercritical Fluids
- volume
- 45
- issue
- 1
- pages
- 51 - 56
- publisher
- Elsevier
- external identifiers
-
- wos:000255297400006
- scopus:40949108080
- ISSN
- 0896-8446
- DOI
- 10.1016/j.supflu.2007.11.011
- language
- English
- LU publication?
- yes
- id
- 757e3560-2db8-4142-96b9-c08f8d4a15dd (old id 1205253)
- date added to LUP
- 2016-04-01 12:16:54
- date last changed
- 2025-01-02 12:14:16
@article{757e3560-2db8-4142-96b9-c08f8d4a15dd, abstract = {{The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.}}, author = {{Rybka, Jacek and Grycko, Pawel and da Cruz Francisco, José and Gamian, Andrzej and Dey, Estera}}, issn = {{0896-8446}}, keywords = {{vaccine; Salmonella; lipopolysaccharides (LPS); supercritical carbon dioxide (scCO(2))}}, language = {{eng}}, number = {{1}}, pages = {{51--56}}, publisher = {{Elsevier}}, series = {{Journal of Supercritical Fluids}}, title = {{Application of supercritical carbon dioxide(scCO(2))for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp enterica PCM 2266}}, url = {{http://dx.doi.org/10.1016/j.supflu.2007.11.011}}, doi = {{10.1016/j.supflu.2007.11.011}}, volume = {{45}}, year = {{2008}}, }