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Lipase-catalysed enrichment of DHA and EPA in acylglycerols resulting from squid oil ethanolysis

Lyberg, Ann-Marie LU and Adlercreutz, Patrick LU (2008) In European Journal of Lipid Science and Technology 110(4). p.317-324
Abstract
The fatty acid specificity of four lipases towards eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was evaluated when performing ethanolysis of squid oil. During the first part of ethanolysis, no DHA ethyl esters were detected when using the lipases from Thermomyces lanuginosus, Pseudomonas cepacia or Pseudomonas fluorescens (in the case of the second and third lipases, no EPA ethyl esters were detected either). This indicates that these three lipases could not catalyse the conversion of DHA located in a triacylglycerol to ethyl ester, and that the Pseudomonas lipases could not catalyse the conversion of EPA either. This pattern was not found for the lipase from Rhizomucor miehei. The lipase from Thermomyces lanuginosus showed... (More)
The fatty acid specificity of four lipases towards eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was evaluated when performing ethanolysis of squid oil. During the first part of ethanolysis, no DHA ethyl esters were detected when using the lipases from Thermomyces lanuginosus, Pseudomonas cepacia or Pseudomonas fluorescens (in the case of the second and third lipases, no EPA ethyl esters were detected either). This indicates that these three lipases could not catalyse the conversion of DHA located in a triacylglycerol to ethyl ester, and that the Pseudomonas lipases could not catalyse the conversion of EPA either. This pattern was not found for the lipase from Rhizomucor miehei. The lipase from Thermomyces lanuginosus showed the lowest specificity towards DHA and the highest DHA recovery during DHA enrichment in the acylglycerol fraction. It was thus used to catalyse the ethanolysis of squid oil on a larger scale. The ethyl esters formed were removed using short-path distillation, resulting in a product containing mainly mono- and diacylglycerols. The product contained 34 mol-% DHA and 17 mol-% EPA, compared with 19 mol-% DHA and 12 mol-% EPA in the original squid oil. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethanolysis, eicosapentaenoic acid, competitive factor, docosahexaenoic acid, lipase
in
European Journal of Lipid Science and Technology
volume
110
issue
4
pages
317 - 324
publisher
John Wiley & Sons
external identifiers
  • wos:000255323000004
  • scopus:54549105296
ISSN
1438-7697
DOI
10.1002/ejlt.200700217
language
English
LU publication?
yes
id
ee682588-768d-4718-b62b-56dabc934b88 (old id 1205304)
date added to LUP
2008-09-18 14:09:48
date last changed
2017-09-17 04:51:41
@article{ee682588-768d-4718-b62b-56dabc934b88,
  abstract     = {The fatty acid specificity of four lipases towards eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was evaluated when performing ethanolysis of squid oil. During the first part of ethanolysis, no DHA ethyl esters were detected when using the lipases from Thermomyces lanuginosus, Pseudomonas cepacia or Pseudomonas fluorescens (in the case of the second and third lipases, no EPA ethyl esters were detected either). This indicates that these three lipases could not catalyse the conversion of DHA located in a triacylglycerol to ethyl ester, and that the Pseudomonas lipases could not catalyse the conversion of EPA either. This pattern was not found for the lipase from Rhizomucor miehei. The lipase from Thermomyces lanuginosus showed the lowest specificity towards DHA and the highest DHA recovery during DHA enrichment in the acylglycerol fraction. It was thus used to catalyse the ethanolysis of squid oil on a larger scale. The ethyl esters formed were removed using short-path distillation, resulting in a product containing mainly mono- and diacylglycerols. The product contained 34 mol-% DHA and 17 mol-% EPA, compared with 19 mol-% DHA and 12 mol-% EPA in the original squid oil.},
  author       = {Lyberg, Ann-Marie and Adlercreutz, Patrick},
  issn         = {1438-7697},
  keyword      = {ethanolysis,eicosapentaenoic acid,competitive factor,docosahexaenoic acid,lipase},
  language     = {eng},
  number       = {4},
  pages        = {317--324},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Lipid Science and Technology},
  title        = {Lipase-catalysed enrichment of DHA and EPA in acylglycerols resulting from squid oil ethanolysis},
  url          = {http://dx.doi.org/10.1002/ejlt.200700217},
  volume       = {110},
  year         = {2008},
}