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Oxytocin mRNA content in the endometrium of non-pregnant women.

Steinwall, Margareta LU ; Hansson, Stefan LU ; Bossmar, Thomas LU ; Larsson, Irene LU ; Pilka, Radovan and Åkerlund, Mats LU (2004) In BJOG: An International Journal of Obstetrics & Gynaecology2000-01-01+01:00 111(3). p.266-270
Abstract
Objective To study oxytocin mRNA in the human endometrium at different phases of the menstrual cycle.



Design An exploratory study in non-pregnant women.



Setting The Department of Obstetrics and Gynecology, Lund University Hospital, Sweden.



Participants Thirty-three women of fertile age undergoing hysterectomy or endometrial curettage on routine benign gynaecologic indications.



Methods Endometrial tissue was obtained throughout the menstrual cycle. The presence of oxytocin mRNA was investigated by in situ hybridisation and by real time PCR.



Main outcome measures Oxytocin mRNA signalling intensity found by in situ hybridisation of tissue obtained... (More)
Objective To study oxytocin mRNA in the human endometrium at different phases of the menstrual cycle.



Design An exploratory study in non-pregnant women.



Setting The Department of Obstetrics and Gynecology, Lund University Hospital, Sweden.



Participants Thirty-three women of fertile age undergoing hysterectomy or endometrial curettage on routine benign gynaecologic indications.



Methods Endometrial tissue was obtained throughout the menstrual cycle. The presence of oxytocin mRNA was investigated by in situ hybridisation and by real time PCR.



Main outcome measures Oxytocin mRNA signalling intensity found by in situ hybridisation of tissue obtained at different times of the menstrual cycle. Relative amounts of oxytocin mRNA measured by real time PCR.



Results The signal for oxytocin mRNA obtained by in situ hybridisation was more pronounced in glandular epithelial cells than in stromal cells. Furthermore, it was most marked around mid-cycle. The expression of oxytocin mRNA was confirmed by real time PCR.



Conclusions The results indicate that oxytocin may be synthesised in the endometrium of non-pregnant women, particularly in the glandular epithelial cells. Hormone released from these sources may have a paracrine action on the uterus. Oxytocin mRNA expression seems to be ovarian hormone dependent with the highest concentration around mid-cycle. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BJOG: An International Journal of Obstetrics & Gynaecology2000-01-01+01:00
volume
111
issue
3
pages
266 - 270
publisher
Wiley-Blackwell
external identifiers
  • wos:000189354100013
  • pmid:14961889
  • scopus:1542314793
ISSN
1471-0528
DOI
10.1111/j.1471-0528.2004.00049.x
language
English
LU publication?
yes
id
22c20ebe-13b6-453a-805c-1dc17ce2fb38 (old id 120562)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=14961889&dopt=Abstract
date added to LUP
2007-07-18 11:36:42
date last changed
2017-01-01 06:39:35
@article{22c20ebe-13b6-453a-805c-1dc17ce2fb38,
  abstract     = {Objective To study oxytocin mRNA in the human endometrium at different phases of the menstrual cycle.<br/><br>
<br/><br>
Design An exploratory study in non-pregnant women.<br/><br>
<br/><br>
Setting The Department of Obstetrics and Gynecology, Lund University Hospital, Sweden.<br/><br>
<br/><br>
Participants Thirty-three women of fertile age undergoing hysterectomy or endometrial curettage on routine benign gynaecologic indications.<br/><br>
<br/><br>
Methods Endometrial tissue was obtained throughout the menstrual cycle. The presence of oxytocin mRNA was investigated by in situ hybridisation and by real time PCR.<br/><br>
<br/><br>
Main outcome measures Oxytocin mRNA signalling intensity found by in situ hybridisation of tissue obtained at different times of the menstrual cycle. Relative amounts of oxytocin mRNA measured by real time PCR.<br/><br>
<br/><br>
Results The signal for oxytocin mRNA obtained by in situ hybridisation was more pronounced in glandular epithelial cells than in stromal cells. Furthermore, it was most marked around mid-cycle. The expression of oxytocin mRNA was confirmed by real time PCR.<br/><br>
<br/><br>
Conclusions The results indicate that oxytocin may be synthesised in the endometrium of non-pregnant women, particularly in the glandular epithelial cells. Hormone released from these sources may have a paracrine action on the uterus. Oxytocin mRNA expression seems to be ovarian hormone dependent with the highest concentration around mid-cycle.},
  author       = {Steinwall, Margareta and Hansson, Stefan and Bossmar, Thomas and Larsson, Irene and Pilka, Radovan and Åkerlund, Mats},
  issn         = {1471-0528},
  language     = {eng},
  number       = {3},
  pages        = {266--270},
  publisher    = {Wiley-Blackwell},
  series       = {BJOG: An International Journal of Obstetrics & Gynaecology2000-01-01+01:00},
  title        = {Oxytocin mRNA content in the endometrium of non-pregnant women.},
  url          = {http://dx.doi.org/10.1111/j.1471-0528.2004.00049.x},
  volume       = {111},
  year         = {2004},
}