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Amplification of chromosome 1 sequences in lipomatous tumors and other sarcomas

Nilsson, Malin A LU ; Meza-Zepeda, Leonardo A; Mertens, Fredrik LU ; Forus, Anne; Myklebost, Ola and Mandahl, Nils LU (2004) In International Journal of Cancer 109(3). p.363-369
Abstract

Amplifications and gains involving 1q are common abnormalities in solid tumors. Recently, an amplicon originating from 1q21-23, containing the candidate oncogenes COAS1, COAS2 and COAS3 (Chromosome One Amplified Sequence) was identified. The presence, distribution and copy number level of extra COAS sequences were investigated in 48 bone and soft tissue tumor (BSTT) samples using metaphase FISH analysis. Amplification was seen in 27/48 (56%) samples. With few exceptions, all 3 genes were involved, but on average COAS2 exhibited higher copy numbers. The presence of extra COAS signals, irrespective of copy numbers, was found at similar frequencies in different histologic tumor subtypes. However, medium or high level amplification was... (More)

Amplifications and gains involving 1q are common abnormalities in solid tumors. Recently, an amplicon originating from 1q21-23, containing the candidate oncogenes COAS1, COAS2 and COAS3 (Chromosome One Amplified Sequence) was identified. The presence, distribution and copy number level of extra COAS sequences were investigated in 48 bone and soft tissue tumor (BSTT) samples using metaphase FISH analysis. Amplification was seen in 27/48 (56%) samples. With few exceptions, all 3 genes were involved, but on average COAS2 exhibited higher copy numbers. The presence of extra COAS signals, irrespective of copy numbers, was found at similar frequencies in different histologic tumor subtypes. However, medium or high level amplification was common in lipomatous tumors but rare in other, nonlipomatous tumors (9/21 vs. 2/27 samples). The most common localization of extra COAS signals in lipomatous tumors was in supernumerary ring and giant marker chromosomes. Among nonlipomatous tumors, the distribution of extra COAS genes was more disperse, being located in various unidentified chromosomal structures, including double minutes, and only rarely in ring chromosomes. Because MDM2 is known to be amplified frequently in BSTTs, and in particular in atypical lipomatous tumors, cases with extra copies of COAS were studied also with an MDM2 probe. Twelve out of 18 lipomatous tumors had extra copies of both COAS and MDM2, and the 2 genes were found to be coamplified and interspersed exclusively in ring and giant marker chromosomes. Also 12 out of 18 nonlipomatous tumors exhibited simultaneous gain of COAS and MDM2, but colocalization in the same chromosome was less frequent. The role of the frequent coamplification of COAS, or some other yet unknown gene in the 1q21-23 region, and MDM2 remains to be elucidated.

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keywords
Adult, Aged, Bone Neoplasms, Chromosome Banding, Chromosome Mapping, Chromosomes, Human, Pair 1, Female, Gene Amplification, Genes, Tumor Suppressor, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Lipoma, Liposarcoma, Male, Middle Aged, Nuclear Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-mdm2, Sarcoma, Journal Article, Research Support, Non-U.S. Gov't
in
International Journal of Cancer
volume
109
issue
3
pages
7 pages
publisher
John Wiley & Sons
external identifiers
  • wos:000189245300008
  • pmid:14961574
  • scopus:1342267079
ISSN
0020-7136
DOI
10.1002/ijc.11716
language
English
LU publication?
yes
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7cddd611-1c07-4af9-bfcd-7eac53f89912 (old id 120578)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14961574&dopt=Abstract
date added to LUP
2007-07-09 08:37:12
date last changed
2017-10-08 03:38:22
@article{7cddd611-1c07-4af9-bfcd-7eac53f89912,
  abstract     = {<p>Amplifications and gains involving 1q are common abnormalities in solid tumors. Recently, an amplicon originating from 1q21-23, containing the candidate oncogenes COAS1, COAS2 and COAS3 (Chromosome One Amplified Sequence) was identified. The presence, distribution and copy number level of extra COAS sequences were investigated in 48 bone and soft tissue tumor (BSTT) samples using metaphase FISH analysis. Amplification was seen in 27/48 (56%) samples. With few exceptions, all 3 genes were involved, but on average COAS2 exhibited higher copy numbers. The presence of extra COAS signals, irrespective of copy numbers, was found at similar frequencies in different histologic tumor subtypes. However, medium or high level amplification was common in lipomatous tumors but rare in other, nonlipomatous tumors (9/21 vs. 2/27 samples). The most common localization of extra COAS signals in lipomatous tumors was in supernumerary ring and giant marker chromosomes. Among nonlipomatous tumors, the distribution of extra COAS genes was more disperse, being located in various unidentified chromosomal structures, including double minutes, and only rarely in ring chromosomes. Because MDM2 is known to be amplified frequently in BSTTs, and in particular in atypical lipomatous tumors, cases with extra copies of COAS were studied also with an MDM2 probe. Twelve out of 18 lipomatous tumors had extra copies of both COAS and MDM2, and the 2 genes were found to be coamplified and interspersed exclusively in ring and giant marker chromosomes. Also 12 out of 18 nonlipomatous tumors exhibited simultaneous gain of COAS and MDM2, but colocalization in the same chromosome was less frequent. The role of the frequent coamplification of COAS, or some other yet unknown gene in the 1q21-23 region, and MDM2 remains to be elucidated.</p>},
  author       = {Nilsson, Malin A and Meza-Zepeda, Leonardo A and Mertens, Fredrik and Forus, Anne and Myklebost, Ola and Mandahl, Nils},
  issn         = {0020-7136},
  keyword      = {Adult,Aged,Bone Neoplasms,Chromosome Banding,Chromosome Mapping,Chromosomes, Human, Pair 1,Female,Gene Amplification,Genes, Tumor Suppressor,Humans,In Situ Hybridization, Fluorescence,Karyotyping,Lipoma,Liposarcoma,Male,Middle Aged,Nuclear Proteins,Proto-Oncogene Proteins,Proto-Oncogene Proteins c-mdm2,Sarcoma,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {04},
  number       = {3},
  pages        = {363--369},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Amplification of chromosome 1 sequences in lipomatous tumors and other sarcomas},
  url          = {http://dx.doi.org/10.1002/ijc.11716},
  volume       = {109},
  year         = {2004},
}