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Neuronal and glial differentiation within expanded glial cultures derived from the lateral and medial ganglionic eminences.

Eriksson Linsmeier, Cecilia LU and Wictorin, Klas LU (2003) In Experimental Neurology 184(2). p.1058-1063
Abstract
Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of βIII-tubulin-positive neurons were generated in both the LGE (about 7% of total cell numbers) and the MGE (around 2%). However, only few βIII-tubulin-positive cells with neuronal morphologies were detected in the differentiated Cx cultures. The newly formed neurons were to a large extent GABAergic, and many of the MGE-derived, but not the LGE-derived, cells... (More)
Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of βIII-tubulin-positive neurons were generated in both the LGE (about 7% of total cell numbers) and the MGE (around 2%). However, only few βIII-tubulin-positive cells with neuronal morphologies were detected in the differentiated Cx cultures. The newly formed neurons were to a large extent GABAergic, and many of the MGE-derived, but not the LGE-derived, cells expressed the MGE-marker NKX2.1. Most cells in all cultures still appeared astroglial-like, expressing glial fibrillary acidic protein (GFAP), but in addition, CNPase-positive cells with oligodendroglial morphologies were present in the MGE (0.68%), and, to a lesser extent (0.2%), in the LGE cultures. The present results demonstrate that cells of expanded glial cultures from both the LGE and MGE can give rise to significant and, to a certain extent, region-specific neuronal and glial cell types under differentiating conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Differentiation, Expansion, EGF, Astroglial cells, Telencephalon, Neurons, Oligodendrocytes
in
Experimental Neurology
volume
184
issue
2
pages
1058 - 1063
publisher
Academic Press
external identifiers
  • wos:000187634400055
  • pmid:14769401
  • scopus:0842277722
ISSN
0014-4886
DOI
10.1016/j.expneurol.2003.08.014
language
English
LU publication?
yes
id
d8733036-8457-45cd-80bd-2062cc10ad71 (old id 120655)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14769401&dopt=Abstract
date added to LUP
2007-07-12 08:30:58
date last changed
2018-10-03 10:50:20
@article{d8733036-8457-45cd-80bd-2062cc10ad71,
  abstract     = {Attached glial-like cell cultures were established from the lateral and medial ganglionic eminences (LGE and MGE) and from the neocortex (Cx) of E13.5 mouse embryos, and expanded over four to five passages under epidermal growth factor (EGF) stimulation. Following removal of EGF and serum, we analysed the generation of neurons and glial cells within the cultures. Significant numbers of βIII-tubulin-positive neurons were generated in both the LGE (about 7% of total cell numbers) and the MGE (around 2%). However, only few βIII-tubulin-positive cells with neuronal morphologies were detected in the differentiated Cx cultures. The newly formed neurons were to a large extent GABAergic, and many of the MGE-derived, but not the LGE-derived, cells expressed the MGE-marker NKX2.1. Most cells in all cultures still appeared astroglial-like, expressing glial fibrillary acidic protein (GFAP), but in addition, CNPase-positive cells with oligodendroglial morphologies were present in the MGE (0.68%), and, to a lesser extent (0.2%), in the LGE cultures. The present results demonstrate that cells of expanded glial cultures from both the LGE and MGE can give rise to significant and, to a certain extent, region-specific neuronal and glial cell types under differentiating conditions.},
  author       = {Eriksson Linsmeier, Cecilia and Wictorin, Klas},
  issn         = {0014-4886},
  keyword      = {Differentiation,Expansion,EGF,Astroglial cells,Telencephalon,Neurons,Oligodendrocytes},
  language     = {eng},
  number       = {2},
  pages        = {1058--1063},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Neuronal and glial differentiation within expanded glial cultures derived from the lateral and medial ganglionic eminences.},
  url          = {http://dx.doi.org/10.1016/j.expneurol.2003.08.014},
  volume       = {184},
  year         = {2003},
}