Advanced

The early ontogeny of neuronal nitric oxide synthase systems in the zebrafish.

Holmqvist, Bo LU ; Ellingsen, B; Forsell, Johan LU ; Zhdanova, I and Alm, Per LU (2004) In Journal of Experimental Biology 207(Pt 6). p.923-935
Abstract
To examine a putative role for neuronal nitric oxide synthase (nNOS) in early vertebrate development we investigated nNOS mRNA expression and cGMP production during development of the zebrafish Danio rerio. The nNOS mRNA expression in the central nervous system (CNS) and periphery showed a distinct spatio-temporal pattern in developing zebrafish embryo and young larvae. nNOS mRNA expression was first detected at 19 h postfertilisation (h.p.f.), in a bilateral subpopulation of the embryonic ventrorostral cell cluster in the forebrain. The number of nNOS mRNA-expressing cells in the brain slowly increased, also appearing in the ventrocaudal cell cluster from about 26 h.p.f., and in the dorsorostral and hindbrain cell cluster and in the... (More)
To examine a putative role for neuronal nitric oxide synthase (nNOS) in early vertebrate development we investigated nNOS mRNA expression and cGMP production during development of the zebrafish Danio rerio. The nNOS mRNA expression in the central nervous system (CNS) and periphery showed a distinct spatio-temporal pattern in developing zebrafish embryo and young larvae. nNOS mRNA expression was first detected at 19 h postfertilisation (h.p.f.), in a bilateral subpopulation of the embryonic ventrorostral cell cluster in the forebrain. The number of nNOS mRNA-expressing cells in the brain slowly increased, also appearing in the ventrocaudal cell cluster from about 26 h.p.f., and in the dorsorostral and hindbrain cell cluster and in the medulla at 30 h.p.f. A major increase in nNOS mRNA expression started at about 40 h.p.f., and by 55 h.p.f. the expression constituted cell populations in differentiated central nuclei and in association with the proliferation zones of the brain, and in the medulla and retina. In parts of the skin, nNOS mRNA expression started at 20 h.p.f. and ended at 55 h.p.f. Between 40 and 55 h.p.f., nNOS mRNA expression started in peripheral organs, forming distinct populations after hatching within or in the vicinity of the presumptive swim bladder, enteric ganglia, and along the alimentary tract and nephritic ducts. Expression of nNOS mRNA correlated with the neuronal differentiation pattern and with the timing and degree of cGMP production. These studies indicate spatio-temporal actions by NO during embryogenesis in the formation of the central and peripheral nervous system, with possible involvement in processes such as neurogenesis, organogenesis and early physiology. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
morphogenesis, Danio rerio, brain, retina, gut, neuronal, regeneration, differentiation, development, in situ, hybridisation, intestine, zebrafish
in
Journal of Experimental Biology
volume
207
issue
Pt 6
pages
923 - 935
publisher
The Company of Biologists Ltd
external identifiers
  • pmid:14766951
  • wos:000220336500009
  • scopus:1642358516
ISSN
1477-9145
DOI
10.1242/jeb.00845
language
English
LU publication?
yes
id
73414caa-9975-4737-bf42-e085f044f99f (old id 120674)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14766951&dopt=Abstract
date added to LUP
2007-07-19 15:43:12
date last changed
2017-09-10 03:35:58
@article{73414caa-9975-4737-bf42-e085f044f99f,
  abstract     = {To examine a putative role for neuronal nitric oxide synthase (nNOS) in early vertebrate development we investigated nNOS mRNA expression and cGMP production during development of the zebrafish Danio rerio. The nNOS mRNA expression in the central nervous system (CNS) and periphery showed a distinct spatio-temporal pattern in developing zebrafish embryo and young larvae. nNOS mRNA expression was first detected at 19 h postfertilisation (h.p.f.), in a bilateral subpopulation of the embryonic ventrorostral cell cluster in the forebrain. The number of nNOS mRNA-expressing cells in the brain slowly increased, also appearing in the ventrocaudal cell cluster from about 26 h.p.f., and in the dorsorostral and hindbrain cell cluster and in the medulla at 30 h.p.f. A major increase in nNOS mRNA expression started at about 40 h.p.f., and by 55 h.p.f. the expression constituted cell populations in differentiated central nuclei and in association with the proliferation zones of the brain, and in the medulla and retina. In parts of the skin, nNOS mRNA expression started at 20 h.p.f. and ended at 55 h.p.f. Between 40 and 55 h.p.f., nNOS mRNA expression started in peripheral organs, forming distinct populations after hatching within or in the vicinity of the presumptive swim bladder, enteric ganglia, and along the alimentary tract and nephritic ducts. Expression of nNOS mRNA correlated with the neuronal differentiation pattern and with the timing and degree of cGMP production. These studies indicate spatio-temporal actions by NO during embryogenesis in the formation of the central and peripheral nervous system, with possible involvement in processes such as neurogenesis, organogenesis and early physiology.},
  author       = {Holmqvist, Bo and Ellingsen, B and Forsell, Johan and Zhdanova, I and Alm, Per},
  issn         = {1477-9145},
  keyword      = {morphogenesis,Danio rerio,brain,retina,gut,neuronal,regeneration,differentiation,development,in situ,hybridisation,intestine,zebrafish},
  language     = {eng},
  number       = {Pt 6},
  pages        = {923--935},
  publisher    = {The Company of Biologists Ltd},
  series       = {Journal of Experimental Biology},
  title        = {The early ontogeny of neuronal nitric oxide synthase systems in the zebrafish.},
  url          = {http://dx.doi.org/10.1242/jeb.00845},
  volume       = {207},
  year         = {2004},
}