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The effect of Tween-20 on simultaneous saccharification and fermentation of softwood to ethanol

Alkasrawi, Malek LU ; Eriksson, Torny LU ; Börjesson, Johan LU ; Wingren, Anders LU ; Galbe, Mats LU ; Tjerneld, Folke LU and Zacchi, Guido LU (2003) In Enzyme and Microbial Technology 33(1). p.71-78
Abstract
Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time... (More)
Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time required to attain maximum ethanol concentration was reduced. Surfactants as an additive in SSF can significantly lower the operational cost of the process. However, less expensive surfactants must be investigated. (C) 2003 Elsevier Science Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethanol production, SSF, Tween-20, enzyme recovery, softwood
in
Enzyme and Microbial Technology
volume
33
issue
1
pages
71 - 78
publisher
Elsevier
external identifiers
  • wos:000183967800010
  • scopus:0037666266
ISSN
0141-0229
DOI
10.1016/S0141-0229(03)00087-5
language
English
LU publication?
yes
id
a95bc4d2-a121-4779-abd8-7572dab80298 (old id 120863)
date added to LUP
2007-06-25 09:42:06
date last changed
2018-10-14 03:39:00
@article{a95bc4d2-a121-4779-abd8-7572dab80298,
  abstract     = {Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time required to attain maximum ethanol concentration was reduced. Surfactants as an additive in SSF can significantly lower the operational cost of the process. However, less expensive surfactants must be investigated. (C) 2003 Elsevier Science Inc. All rights reserved.},
  author       = {Alkasrawi, Malek and Eriksson, Torny and Börjesson, Johan and Wingren, Anders and Galbe, Mats and Tjerneld, Folke and Zacchi, Guido},
  issn         = {0141-0229},
  keyword      = {ethanol production,SSF,Tween-20,enzyme recovery,softwood},
  language     = {eng},
  number       = {1},
  pages        = {71--78},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {The effect of Tween-20 on simultaneous saccharification and fermentation of softwood to ethanol},
  url          = {http://dx.doi.org/10.1016/S0141-0229(03)00087-5},
  volume       = {33},
  year         = {2003},
}