The effect of Tween-20 on simultaneous saccharification and fermentation of softwood to ethanol
(2003) In Enzyme and Microbial Technology 33(1). p.71-78- Abstract
- Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time... (More)
- Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time required to attain maximum ethanol concentration was reduced. Surfactants as an additive in SSF can significantly lower the operational cost of the process. However, less expensive surfactants must be investigated. (C) 2003 Elsevier Science Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/120863
- author
- Alkasrawi, Malek LU ; Eriksson, Torny LU ; Börjesson, Johan LU ; Wingren, Anders LU ; Galbe, Mats LU ; Tjerneld, Folke LU and Zacchi, Guido LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ethanol production, SSF, Tween-20, enzyme recovery, softwood
- in
- Enzyme and Microbial Technology
- volume
- 33
- issue
- 1
- pages
- 71 - 78
- publisher
- Elsevier
- external identifiers
-
- wos:000183967800010
- scopus:0037666266
- ISSN
- 0141-0229
- DOI
- 10.1016/S0141-0229(03)00087-5
- language
- English
- LU publication?
- yes
- id
- a95bc4d2-a121-4779-abd8-7572dab80298 (old id 120863)
- date added to LUP
- 2016-04-01 12:33:13
- date last changed
- 2023-11-26 17:49:29
@article{a95bc4d2-a121-4779-abd8-7572dab80298, abstract = {{Simultaneous sacchatification and fermentation (SSF) of steam-pretreated wood constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition in SSF contributes to a high process cost. In this study we explore the effect of the non-ionic surfactant Tween-20 as an additive in SSE Tween-20 addition at 2.5 g/l had several positive effects on SSF: (i) the ethanol yield was increased by 8%; (ii) the amount of enzyme loading could be reduced by 50%, while maintaining a constant yield; (iii) the enzyme activity increased in the liquid fraction at the end of SSF, probably by preventing unproductive binding of the cellulases to lignin, which could facilitate enzyme recovery; (iv) the time required to attain maximum ethanol concentration was reduced. Surfactants as an additive in SSF can significantly lower the operational cost of the process. However, less expensive surfactants must be investigated. (C) 2003 Elsevier Science Inc. All rights reserved.}}, author = {{Alkasrawi, Malek and Eriksson, Torny and Börjesson, Johan and Wingren, Anders and Galbe, Mats and Tjerneld, Folke and Zacchi, Guido}}, issn = {{0141-0229}}, keywords = {{ethanol production; SSF; Tween-20; enzyme recovery; softwood}}, language = {{eng}}, number = {{1}}, pages = {{71--78}}, publisher = {{Elsevier}}, series = {{Enzyme and Microbial Technology}}, title = {{The effect of Tween-20 on simultaneous saccharification and fermentation of softwood to ethanol}}, url = {{http://dx.doi.org/10.1016/S0141-0229(03)00087-5}}, doi = {{10.1016/S0141-0229(03)00087-5}}, volume = {{33}}, year = {{2003}}, }