Advanced

Antimicrobial activities of heparin-binding peptides.

Andersson, Emma; Rydengård, Victoria LU ; Sonesson, Andreas LU ; Mörgelin, Matthias LU ; Björck, Lars LU and Schmidtchen, Artur LU (2004) In European Journal of Biochemistry 271(6). p.1219-1226
Abstract
Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human antimicrobial peptides alpha-defensin and LL-37 bind to glycosaminoglycans (heparin and dermatan sulphate). Here we demonstrate the obverse, i.e. structural motifs associated with heparin affinity (cationicity, amphipaticity, and consensus regions) may confer antimicrobial properties to a given peptide. Thus, heparin-binding peptides derived from laminin isoforms, von Willebrand factor, vitronectin, protein C inhibitor, and fibronectin, exerted antimicrobial activities against Gram-positive and Gram-negative bacteria. Similar results were obtained using heparin-binding peptides derived from complement factor C3 as well as consensus... (More)
Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human antimicrobial peptides alpha-defensin and LL-37 bind to glycosaminoglycans (heparin and dermatan sulphate). Here we demonstrate the obverse, i.e. structural motifs associated with heparin affinity (cationicity, amphipaticity, and consensus regions) may confer antimicrobial properties to a given peptide. Thus, heparin-binding peptides derived from laminin isoforms, von Willebrand factor, vitronectin, protein C inhibitor, and fibronectin, exerted antimicrobial activities against Gram-positive and Gram-negative bacteria. Similar results were obtained using heparin-binding peptides derived from complement factor C3 as well as consensus sequences for heparin-binding (Cardin and Weintraub motifs). These sequence motifs, and additional peptides, also killed the fungus Candida albicans. These data will have implications for the search for novel antimicrobial peptides and utilization of heparin-protein interactions should be helpful in the identification and purification of novel antimicrobial peptides from complex biological mixtures. Finally, consensus regions may serve as templates for de novo synthesis of novel antimicrobial molecules. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
heparin binding, defensin, antimicrobial, cathelicidin, glycosaminoglycan
in
European Journal of Biochemistry
volume
271
issue
6
pages
1219 - 1226
publisher
Wiley-Blackwell
external identifiers
  • wos:000220006500016
  • pmid:15009200
  • scopus:1642281222
ISSN
0014-2956
DOI
10.1111/j.1432-1033.2004.04035.x
language
English
LU publication?
yes
id
01b74850-44fd-450e-bff8-cb120ab89be6 (old id 121424)
date added to LUP
2007-06-26 08:54:25
date last changed
2017-10-01 04:43:40
@article{01b74850-44fd-450e-bff8-cb120ab89be6,
  abstract     = {Antimicrobial peptides are effector molecules of the innate immune system. We recently showed that the human antimicrobial peptides alpha-defensin and LL-37 bind to glycosaminoglycans (heparin and dermatan sulphate). Here we demonstrate the obverse, i.e. structural motifs associated with heparin affinity (cationicity, amphipaticity, and consensus regions) may confer antimicrobial properties to a given peptide. Thus, heparin-binding peptides derived from laminin isoforms, von Willebrand factor, vitronectin, protein C inhibitor, and fibronectin, exerted antimicrobial activities against Gram-positive and Gram-negative bacteria. Similar results were obtained using heparin-binding peptides derived from complement factor C3 as well as consensus sequences for heparin-binding (Cardin and Weintraub motifs). These sequence motifs, and additional peptides, also killed the fungus Candida albicans. These data will have implications for the search for novel antimicrobial peptides and utilization of heparin-protein interactions should be helpful in the identification and purification of novel antimicrobial peptides from complex biological mixtures. Finally, consensus regions may serve as templates for de novo synthesis of novel antimicrobial molecules.},
  author       = {Andersson, Emma and Rydengård, Victoria and Sonesson, Andreas and Mörgelin, Matthias and Björck, Lars and Schmidtchen, Artur},
  issn         = {0014-2956},
  keyword      = {heparin
binding,defensin,antimicrobial,cathelicidin,glycosaminoglycan},
  language     = {eng},
  number       = {6},
  pages        = {1219--1226},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {Antimicrobial activities of heparin-binding peptides.},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.2004.04035.x},
  volume       = {271},
  year         = {2004},
}