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DNA compaction at hydrophobic surfaces induced by a cationic amphiphile

Cárdenas, Marité LU ; Braem, Alan LU ; Nylander, Tommy LU and Lindman, Björn LU (2003) In Langmuir 19(19). p.7712-7718
Abstract
Cetyltrimethylammonium bromide (CTAB) induces partially irreversible compaction of DNA-adsorbed layers on hydrophobic silica. Additionally, there is a synergistic increase in the adsorbed amount when both CTAB and DNA are present as compared to the surface excess concentration of either of the individual components. In this study of the DNA adsorption and DNA-CTAB coadsorption by ellipsometry, emphasis has been placed on the DNA molecular weight as well as its conformation (single and double stranded). The DNA molecular weight and conformation have a large effect on the surfactant-free DNA adsorption behavior but not on

he mixed DNA-CTAB adsorption behavior. Comparison between interfacial and bulk

complexation has been... (More)
Cetyltrimethylammonium bromide (CTAB) induces partially irreversible compaction of DNA-adsorbed layers on hydrophobic silica. Additionally, there is a synergistic increase in the adsorbed amount when both CTAB and DNA are present as compared to the surface excess concentration of either of the individual components. In this study of the DNA adsorption and DNA-CTAB coadsorption by ellipsometry, emphasis has been placed on the DNA molecular weight as well as its conformation (single and double stranded). The DNA molecular weight and conformation have a large effect on the surfactant-free DNA adsorption behavior but not on

he mixed DNA-CTAB adsorption behavior. Comparison between interfacial and bulk

complexation has been made where possible. The DNA-CTAB complexes at

the interface are neutral despite the large excess of DNA in the

bulk. The final structure of the adsorbed layer was found to be

dependent on the history of complex formation and DNA

size. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
19
issue
19
pages
7712 - 7718
publisher
The American Chemical Society
external identifiers
  • wos:000185293500005
  • scopus:0141508905
ISSN
0743-7463
DOI
10.1021/la026747f
language
English
LU publication?
yes
id
a7017d44-5202-483f-99bf-7ac6da8e0d68 (old id 121954)
date added to LUP
2007-07-11 08:32:31
date last changed
2018-01-07 05:45:40
@article{a7017d44-5202-483f-99bf-7ac6da8e0d68,
  abstract     = {Cetyltrimethylammonium bromide (CTAB) induces partially irreversible compaction of DNA-adsorbed layers on hydrophobic silica. Additionally, there is a synergistic increase in the adsorbed amount when both CTAB and DNA are present as compared to the surface excess concentration of either of the individual components. In this study of the DNA adsorption and DNA-CTAB coadsorption by ellipsometry, emphasis has been placed on the DNA molecular weight as well as its conformation (single and double stranded). The DNA molecular weight and conformation have a large effect on the surfactant-free DNA adsorption behavior but not on <br/><br>
he mixed DNA-CTAB adsorption behavior. Comparison between interfacial and bulk<br/><br>
	complexation has been made where possible. The DNA-CTAB complexes at<br/><br>
	the interface are neutral despite the large excess of DNA in the<br/><br>
	bulk. The final structure of the adsorbed layer was found to be<br/><br>
	dependent on the history of complex formation and DNA<br/><br>
	size.},
  author       = {Cárdenas, Marité and Braem, Alan and Nylander, Tommy and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {19},
  pages        = {7712--7718},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {DNA compaction at hydrophobic surfaces induced by a cationic amphiphile},
  url          = {http://dx.doi.org/10.1021/la026747f},
  volume       = {19},
  year         = {2003},
}