Advanced

Quantification, of photosystem I and II in different parts of the thylakoid membrane from spinach

Danielsson, Ravi LU ; Albertsson, Per-Åke LU ; Mamedov, Fikret LU and Styring, Stenbjörn LU (2004) In Biochimica et Biophysica Acta - Bioenergetics 1608(1). p.53-61
Abstract
Abstract:

Electron paramagnetic resonance (EPR) was used to quantify Photosystem I (PSI) and PSII in vesicles originating from a series of well-defined but different domains of the thylakoid membrane in spinach prepared by non-detergent techniques. Thylakoids from spinach were fragmented by sonication and separated by aqueous polymer two-phase partitioning into vesicles originating from grana and stroma lamellae. The grana vesicles were further sonicated and separated into two vesicle preparations originating from the grana margins and the appressed domains of grana (the grana core), respectively. PSI and PSII were determined in the same samples from the maximal size of the EPR signal from P700(+) and Y-D(.), respectively. The... (More)
Abstract:

Electron paramagnetic resonance (EPR) was used to quantify Photosystem I (PSI) and PSII in vesicles originating from a series of well-defined but different domains of the thylakoid membrane in spinach prepared by non-detergent techniques. Thylakoids from spinach were fragmented by sonication and separated by aqueous polymer two-phase partitioning into vesicles originating from grana and stroma lamellae. The grana vesicles were further sonicated and separated into two vesicle preparations originating from the grana margins and the appressed domains of grana (the grana core), respectively. PSI and PSII were determined in the same samples from the maximal size of the EPR signal from P700(+) and Y-D(.), respectively. The following PSI/PSII ratios were found: thylakoids, 1.13; grana vesicles, 0.43; grana core, 0.25; grana margins, 1.28; stroma lamellae 3.10. In a sub-fraction of the stroma lamellae, denoted Y-100, PSI was highly enriched and the PSI/PSII ratio was 13. The antenna size of the respective photosystems was calculated from the experimental data and the assumption that a PSII center in the stroma lamellae (PSIIbeta) has an antenna size of 100 Ch1. This gave the following results: PSI in grana margins (PSIalpha) 300, PSI (PSIbeta) in stroma lamellae 214, PSII in grana core (PSIIalpha) 280. The results suggest that PSI in grana margins have two additional light-harvesting complex 11 (LHCII) trimers per reaction center compared to PSI in stroma lamellae, and that PSII in grana has four LHCII trimers per monomer compared to PSII in stroma lamellae. Calculation of the total chlorophyll associated with PSI and PSII, respectively, suggests that more chlorophyll (about 10%) is associated with PSI than with PSII. (C) 2003 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
EPR, photosystem antennae, photosystem II, thylakoid membrane, phase partition, photosystem I
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1608
issue
1
pages
53 - 61
publisher
Elsevier
external identifiers
  • wos:000220477500006
  • pmid:14741585
  • scopus:1642559319
ISSN
0005-2728
DOI
10.1016/j.bbabio.2003.10.005
language
English
LU publication?
yes
id
6d38bb41-70a7-4d4a-adad-d280534124a3 (old id 122336)
date added to LUP
2007-07-04 15:56:42
date last changed
2017-12-10 04:27:13
@article{6d38bb41-70a7-4d4a-adad-d280534124a3,
  abstract     = {Abstract:<br/><br>
Electron paramagnetic resonance (EPR) was used to quantify Photosystem I (PSI) and PSII in vesicles originating from a series of well-defined but different domains of the thylakoid membrane in spinach prepared by non-detergent techniques. Thylakoids from spinach were fragmented by sonication and separated by aqueous polymer two-phase partitioning into vesicles originating from grana and stroma lamellae. The grana vesicles were further sonicated and separated into two vesicle preparations originating from the grana margins and the appressed domains of grana (the grana core), respectively. PSI and PSII were determined in the same samples from the maximal size of the EPR signal from P700(+) and Y-D(.), respectively. The following PSI/PSII ratios were found: thylakoids, 1.13; grana vesicles, 0.43; grana core, 0.25; grana margins, 1.28; stroma lamellae 3.10. In a sub-fraction of the stroma lamellae, denoted Y-100, PSI was highly enriched and the PSI/PSII ratio was 13. The antenna size of the respective photosystems was calculated from the experimental data and the assumption that a PSII center in the stroma lamellae (PSIIbeta) has an antenna size of 100 Ch1. This gave the following results: PSI in grana margins (PSIalpha) 300, PSI (PSIbeta) in stroma lamellae 214, PSII in grana core (PSIIalpha) 280. The results suggest that PSI in grana margins have two additional light-harvesting complex 11 (LHCII) trimers per reaction center compared to PSI in stroma lamellae, and that PSII in grana has four LHCII trimers per monomer compared to PSII in stroma lamellae. Calculation of the total chlorophyll associated with PSI and PSII, respectively, suggests that more chlorophyll (about 10%) is associated with PSI than with PSII. (C) 2003 Elsevier B.V. All rights reserved.},
  author       = {Danielsson, Ravi and Albertsson, Per-Åke and Mamedov, Fikret and Styring, Stenbjörn},
  issn         = {0005-2728},
  keyword      = {EPR,photosystem antennae,photosystem II,thylakoid membrane,phase partition,photosystem I},
  language     = {eng},
  number       = {1},
  pages        = {53--61},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {Quantification, of photosystem I and II in different parts of the thylakoid membrane from spinach},
  url          = {http://dx.doi.org/10.1016/j.bbabio.2003.10.005},
  volume       = {1608},
  year         = {2004},
}