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Saccharomyces castellii: A new model organism for telomere research

Astromskas, Eimantas LU (2008)
Abstract
Telomeres are natural ends of eukaryotic chromosomes and perform a major role in cell biology. They protect chromosomes from degradation, fusions and the end replication problem. To study telomeres different model organisms are used including a number of yeast species. In this work I present a new species in yeast telomere studies – Saccharomyces castellii. This yeast exhibits a lot of interesting features that makes this species one of the most promising models for future telomere research. Thus, this work was focused on developing and optimization of genetic assays and tools to make telomere research possible in S. castellii.

We analyzed a number of basic tools and assays that are already used for Saccharomyces cerevisiae... (More)
Telomeres are natural ends of eukaryotic chromosomes and perform a major role in cell biology. They protect chromosomes from degradation, fusions and the end replication problem. To study telomeres different model organisms are used including a number of yeast species. In this work I present a new species in yeast telomere studies – Saccharomyces castellii. This yeast exhibits a lot of interesting features that makes this species one of the most promising models for future telomere research. Thus, this work was focused on developing and optimization of genetic assays and tools to make telomere research possible in S. castellii.

We analyzed a number of basic tools and assays that are already used for Saccharomyces cerevisiae research and found that these tools can be directly applied to study S. castellii genetics. Furthermore, using our newly developed assays we analyzed the structure and properties of S. castellii telomeric 3’ overhangs and found that S. castellii exhibits not only short but also a fraction of long 3’ overhangs.

In summary, we found that S. castellii is a new promising model organism for different studies and especially for telomere biology. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • McEachern, Michael, Associate Professor
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Naumovia castellii, Saccharomyces castellii, yeast, genetic tools, gene targeting, telomere, CDC13., telomeric overhang
pages
112 pages
defense location
Sölvegatan 35, Lund
defense date
2008-10-08 10:00
ISBN
978-91-85067-47-3
language
English
LU publication?
yes
id
ecf960d5-3502-4410-825f-7bf0f57f17ab (old id 1233223)
date added to LUP
2008-09-12 10:34:05
date last changed
2016-09-19 08:45:19
@phdthesis{ecf960d5-3502-4410-825f-7bf0f57f17ab,
  abstract     = {Telomeres are natural ends of eukaryotic chromosomes and perform a major role in cell biology. They protect chromosomes from degradation, fusions and the end replication problem. To study telomeres different model organisms are used including a number of yeast species. In this work I present a new species in yeast telomere studies – Saccharomyces castellii. This yeast exhibits a lot of interesting features that makes this species one of the most promising models for future telomere research. Thus, this work was focused on developing and optimization of genetic assays and tools to make telomere research possible in S. castellii. <br/><br>
We analyzed a number of basic tools and assays that are already used for Saccharomyces cerevisiae research and found that these tools can be directly applied to study S. castellii genetics. Furthermore, using our newly developed assays we analyzed the structure and properties of S. castellii telomeric 3’ overhangs and found that S. castellii exhibits not only short but also a fraction of long 3’ overhangs. <br/><br>
In summary, we found that S. castellii is a new promising model organism for different studies and especially for telomere biology.},
  author       = {Astromskas, Eimantas},
  isbn         = {978-91-85067-47-3},
  keyword      = {Naumovia castellii,Saccharomyces castellii,yeast,genetic tools,gene targeting,telomere,CDC13.,telomeric overhang},
  language     = {eng},
  pages        = {112},
  school       = {Lund University},
  title        = {Saccharomyces castellii: A new model organism for telomere research},
  year         = {2008},
}