Quantitative interaction effects of carbon dioxide, sodium chloride, and sodium nitrite on neurotoxin gene expression in nonproteolytic Clostridium botulinum type B.
(2004) In Applied and Environmental Microbiology 70(5). p.2928-2934- Abstract
- The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a tryptone-peptone-yeast extract (TPY) medium. Various concentrations of these selected food preservatives were studied by using a complete factorial design in order to quantitatively study interaction effects, as well as main effects, on the following responses: lag phase duration (LPD), growth rate, relative cntB expression, and extracellular BoNT/B production. Multiple linear regression was used to set up six statistical models to quantify and predict these responses. All combinations of NaCl and NaNO2 in the growth medium resulted in a prolonged... (More)
- The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a tryptone-peptone-yeast extract (TPY) medium. Various concentrations of these selected food preservatives were studied by using a complete factorial design in order to quantitatively study interaction effects, as well as main effects, on the following responses: lag phase duration (LPD), growth rate, relative cntB expression, and extracellular BoNT/B production. Multiple linear regression was used to set up six statistical models to quantify and predict these responses. All combinations of NaCl and NaNO2 in the growth medium resulted in a prolonged lag phase duration and in a reduction in the specific growth rate. In contrast, the relative BoNT/B gene expression was unchanged, as determined by the cntB-specific quantitative reverse transcription-PCR method. This was confirmed when we measured the extracellular BoNT/B concentration by an enzyme-linked immunosorbent assay. CO2 was found to have a major effect on gene expression when the cntB mRNA levels were monitored in the mid-exponential, late exponential, and late stationary growth phases. The expression of cntB relative to the expression of the 16S rRNA gene was stimulated by an elevated CO2 concentration; the cntB mRNA level was fivefold greater in a 70% CO2 atmosphere than in a 10% CO2 atmosphere. These findings were also confirmed when we analyzed the extracellular BoNT/B concentration; we found that the concentrations were 27 ng · ml–1 · unit of optical density–1 in the 10% CO2 atmosphere and 126 ng · ml–1 · unit of optical density–1 in the 70% CO2 atmosphere. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/123611
- author
- Lövenklev, Maria LU ; Artin, Ingrid LU ; Hagberg, Oskar LU ; Borch, Elisabeth ; Holst, Elisabet LU and Rådström, Peter LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 70
- issue
- 5
- pages
- 2928 - 2934
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000221340400049
- pmid:15128553
- scopus:2442701962
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.70.5.2928-2934.2004
- language
- English
- LU publication?
- yes
- id
- e6db9458-567a-4de2-9a69-fd844d7cee8e (old id 123611)
- date added to LUP
- 2016-04-01 11:37:36
- date last changed
- 2022-01-26 07:48:44
@article{e6db9458-567a-4de2-9a69-fd844d7cee8e,
abstract = {{The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a tryptone-peptone-yeast extract (TPY) medium. Various concentrations of these selected food preservatives were studied by using a complete factorial design in order to quantitatively study interaction effects, as well as main effects, on the following responses: lag phase duration (LPD), growth rate, relative cntB expression, and extracellular BoNT/B production. Multiple linear regression was used to set up six statistical models to quantify and predict these responses. All combinations of NaCl and NaNO2 in the growth medium resulted in a prolonged lag phase duration and in a reduction in the specific growth rate. In contrast, the relative BoNT/B gene expression was unchanged, as determined by the cntB-specific quantitative reverse transcription-PCR method. This was confirmed when we measured the extracellular BoNT/B concentration by an enzyme-linked immunosorbent assay. CO2 was found to have a major effect on gene expression when the cntB mRNA levels were monitored in the mid-exponential, late exponential, and late stationary growth phases. The expression of cntB relative to the expression of the 16S rRNA gene was stimulated by an elevated CO2 concentration; the cntB mRNA level was fivefold greater in a 70% CO2 atmosphere than in a 10% CO2 atmosphere. These findings were also confirmed when we analyzed the extracellular BoNT/B concentration; we found that the concentrations were 27 ng · ml–1 · unit of optical density–1 in the 10% CO2 atmosphere and 126 ng · ml–1 · unit of optical density–1 in the 70% CO2 atmosphere.}},
author = {{Lövenklev, Maria and Artin, Ingrid and Hagberg, Oskar and Borch, Elisabeth and Holst, Elisabet and Rådström, Peter}},
issn = {{0099-2240}},
language = {{eng}},
number = {{5}},
pages = {{2928--2934}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Quantitative interaction effects of carbon dioxide, sodium chloride, and sodium nitrite on neurotoxin gene expression in nonproteolytic Clostridium botulinum type B.}},
url = {{https://lup.lub.lu.se/search/files/2566806/624016.pdf}},
doi = {{10.1128/AEM.70.5.2928-2934.2004}},
volume = {{70}},
year = {{2004}},
}