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Contrasting Short-Term Antibiotic Effects on Respiration and Bacterial Growth Compromises the Validity of the Selective Respiratory Inhibition Technique to Distinguish Fungi and Bacteria.

Rousk, Johannes LU ; Aldén, Louise LU and Bååth, Erland LU (2009) In Microbial Ecology 58(1). p.75-85
Abstract
The selective inhibition (SI) technique has been widely used to resolve fungal and bacterial biomass. By studying bacterial growth (leucine/thymidine incorporation) and respiration simultaneously, this study demonstrates that the inhibitors the SI technique is based on do not efficiently or specifically resolve fungal and bacterial contributions to respiration. At concentrations that completely inhibited bacterial growth, the bactericide streptomycin had no influence on the SI technique's respiration measurement, and complete inhibition of bacterial growth using oxytetracycline resulted in marginal respiration reductions. The fungicides captan and benomyl severely inhibited non-target bacterial growth. Cycloheximide did not reduce... (More)
The selective inhibition (SI) technique has been widely used to resolve fungal and bacterial biomass. By studying bacterial growth (leucine/thymidine incorporation) and respiration simultaneously, this study demonstrates that the inhibitors the SI technique is based on do not efficiently or specifically resolve fungal and bacterial contributions to respiration. At concentrations that completely inhibited bacterial growth, the bactericide streptomycin had no influence on the SI technique's respiration measurement, and complete inhibition of bacterial growth using oxytetracycline resulted in marginal respiration reductions. The fungicides captan and benomyl severely inhibited non-target bacterial growth. Cycloheximide did not reduce bacterial growth at moderate concentrations, but the cycloheximide respiration reduction was no higher in a soil with more fungal biomass, casting doubt on its ability to discriminate fungal respiration contribution. Conclusions regarding bacteria and fungi based on the SI technique using these inhibitors are thus compromised. The inhibition of glucose-activated respiration by the bactericide bronopol appeared to correlate with bacterial growth inhibition, however. Bronopol, combined with growth-based techniques, could aid development of a new framework to resolve decomposer ecology in soil. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Ecology
volume
58
issue
1
pages
75 - 85
publisher
Springer
external identifiers
  • wos:000266913700008
  • scopus:67649174179
ISSN
1432-184X
DOI
10.1007/s00248-008-9444-1
project
Effect of environmental factors on fungal and bacterial growth in soil
Interaction between fungi and bacteria in soil
Microbial carbon-use efficiency
language
English
LU publication?
yes
id
78932fda-1c22-49df-8db3-ecbf4b529265 (old id 1242915)
date added to LUP
2008-10-22 15:38:47
date last changed
2017-07-30 04:01:46
@article{78932fda-1c22-49df-8db3-ecbf4b529265,
  abstract     = {The selective inhibition (SI) technique has been widely used to resolve fungal and bacterial biomass. By studying bacterial growth (leucine/thymidine incorporation) and respiration simultaneously, this study demonstrates that the inhibitors the SI technique is based on do not efficiently or specifically resolve fungal and bacterial contributions to respiration. At concentrations that completely inhibited bacterial growth, the bactericide streptomycin had no influence on the SI technique's respiration measurement, and complete inhibition of bacterial growth using oxytetracycline resulted in marginal respiration reductions. The fungicides captan and benomyl severely inhibited non-target bacterial growth. Cycloheximide did not reduce bacterial growth at moderate concentrations, but the cycloheximide respiration reduction was no higher in a soil with more fungal biomass, casting doubt on its ability to discriminate fungal respiration contribution. Conclusions regarding bacteria and fungi based on the SI technique using these inhibitors are thus compromised. The inhibition of glucose-activated respiration by the bactericide bronopol appeared to correlate with bacterial growth inhibition, however. Bronopol, combined with growth-based techniques, could aid development of a new framework to resolve decomposer ecology in soil.},
  author       = {Rousk, Johannes and Aldén, Louise and Bååth, Erland},
  issn         = {1432-184X},
  language     = {eng},
  number       = {1},
  pages        = {75--85},
  publisher    = {Springer},
  series       = {Microbial Ecology},
  title        = {Contrasting Short-Term Antibiotic Effects on Respiration and Bacterial Growth Compromises the Validity of the Selective Respiratory Inhibition Technique to Distinguish Fungi and Bacteria.},
  url          = {http://dx.doi.org/10.1007/s00248-008-9444-1},
  volume       = {58},
  year         = {2009},
}