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Effect of polyamine deficiency on proteins involved in Okazaki fragment maturation.

Johansson, Veronica LU ; Falck Miniotis, Maria; Hegardt, Cecilia LU ; Jönsson, Göran B LU ; Staaf, Johan LU ; Berntsson, Pia LU ; Oredsson, Stina LU and Alm, Kersti LU (2008) In Cell Biology International 32. p.1467-1477
Abstract
Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion... (More)
Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion sensitivity, something that can be useful for cancer treatment with polyamine analogues. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CGP 48664, DENSPM, human breast cancer, FEN1, DNA ligase I
in
Cell Biology International
volume
32
pages
1467 - 1477
publisher
Elsevier
external identifiers
  • wos:000261001100001
  • scopus:55949114642
ISSN
1095-8355
DOI
10.1016/j.cellbi.2008.08.018
language
English
LU publication?
yes
id
0daf9ffa-f3ad-4d3b-a803-9e10fb76def5 (old id 1243097)
date added to LUP
2008-11-06 12:12:22
date last changed
2017-01-01 05:07:54
@article{0daf9ffa-f3ad-4d3b-a803-9e10fb76def5,
  abstract     = {Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion sensitivity, something that can be useful for cancer treatment with polyamine analogues. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis.},
  author       = {Johansson, Veronica and Falck Miniotis, Maria and Hegardt, Cecilia and Jönsson, Göran B and Staaf, Johan and Berntsson, Pia and Oredsson, Stina and Alm, Kersti},
  issn         = {1095-8355},
  keyword      = {CGP 48664,DENSPM,human breast cancer,FEN1,DNA ligase I},
  language     = {eng},
  pages        = {1467--1477},
  publisher    = {Elsevier},
  series       = {Cell Biology International},
  title        = {Effect of polyamine deficiency on proteins involved in Okazaki fragment maturation.},
  url          = {http://dx.doi.org/10.1016/j.cellbi.2008.08.018},
  volume       = {32},
  year         = {2008},
}