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Local effects on triiodothyronine-treated polyglactin sutures on regeneration across peripheral nerve defects.

Scherman, Peter LU ; Kanje, Martin LU and Dahlin, Lars LU (2004) In Tissue Engineering 10(3-4). p.455-464
Abstract
We have previously described a new and simple method for nerve repair in which continuous longitudinal polyglactin sutures alone are used to bridge limited nerve defects in rats. Here we examined whether such sutures could be used to deliver a growth-promoting substance, triiodothyronine (T3), and enhance regeneration of the rat sciatic nerve. Sutures were pretreated in highly concentrated solutions of T3 for 24 h. In vitro measurements showed that such sutures released T3 with an initial rapid phase followed by a slow-release phase lasting at least 3 weeks. Bilateral sciatic nerve defects (7 mm) in rats were bridged by either T3- or saline-incubated sutures. Immunocytochemistry for Schwann cells and axons at 2 weeks showed no differences... (More)
We have previously described a new and simple method for nerve repair in which continuous longitudinal polyglactin sutures alone are used to bridge limited nerve defects in rats. Here we examined whether such sutures could be used to deliver a growth-promoting substance, triiodothyronine (T3), and enhance regeneration of the rat sciatic nerve. Sutures were pretreated in highly concentrated solutions of T3 for 24 h. In vitro measurements showed that such sutures released T3 with an initial rapid phase followed by a slow-release phase lasting at least 3 weeks. Bilateral sciatic nerve defects (7 mm) in rats were bridged by either T3- or saline-incubated sutures. Immunocytochemistry for Schwann cells and axons at 2 weeks showed no differences in Schwann cell distribution or axonal outgrowth length. Morphometric analysis 4 and 12 weeks after the repair revealed a slight but significant (p < 0.05) increase in the mean myelin area in T3-treated nerves. No differences were seen in the number of axons or return of force in the gastrocnemius muscle at 12 weeks. The results show that sutures can be used both for the bridging of defects in peripheral nerves and for the delivery of a growth-promoting substance to regenerating nerve structures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Tissue Engineering
volume
10
issue
3-4
pages
455 - 464
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000221105000015
  • scopus:2342503834
ISSN
1076-3279
DOI
10.1089/107632704323061816
language
English
LU publication?
yes
id
f2ec82b3-cc1e-44d8-98e6-299d5838524f (old id 124533)
alternative location
http://www.liebertonline.com/doi/abs/10.1089/107632704323061816
date added to LUP
2007-07-23 13:14:13
date last changed
2017-04-16 04:14:38
@article{f2ec82b3-cc1e-44d8-98e6-299d5838524f,
  abstract     = {We have previously described a new and simple method for nerve repair in which continuous longitudinal polyglactin sutures alone are used to bridge limited nerve defects in rats. Here we examined whether such sutures could be used to deliver a growth-promoting substance, triiodothyronine (T3), and enhance regeneration of the rat sciatic nerve. Sutures were pretreated in highly concentrated solutions of T3 for 24 h. In vitro measurements showed that such sutures released T3 with an initial rapid phase followed by a slow-release phase lasting at least 3 weeks. Bilateral sciatic nerve defects (7 mm) in rats were bridged by either T3- or saline-incubated sutures. Immunocytochemistry for Schwann cells and axons at 2 weeks showed no differences in Schwann cell distribution or axonal outgrowth length. Morphometric analysis 4 and 12 weeks after the repair revealed a slight but significant (p &lt; 0.05) increase in the mean myelin area in T3-treated nerves. No differences were seen in the number of axons or return of force in the gastrocnemius muscle at 12 weeks. The results show that sutures can be used both for the bridging of defects in peripheral nerves and for the delivery of a growth-promoting substance to regenerating nerve structures.},
  author       = {Scherman, Peter and Kanje, Martin and Dahlin, Lars},
  issn         = {1076-3279},
  language     = {eng},
  number       = {3-4},
  pages        = {455--464},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Tissue Engineering},
  title        = {Local effects on triiodothyronine-treated polyglactin sutures on regeneration across peripheral nerve defects.},
  url          = {http://dx.doi.org/10.1089/107632704323061816},
  volume       = {10},
  year         = {2004},
}