Acoustic microfluidic chip technology to facilitate automation of phage display selection
Persson, Jonas LU ; Augustsson, Per LU ; Laurell, Thomas LU and Ohlin, Mats LU
(2008)
In The FEBS Journal
275(22).
p.5657-5666
- Abstract
- Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the... (More)
- Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1245883
- author
- Persson, Jonas
LU
; Augustsson, Per
LU
; Laurell, Thomas
LU
and Ohlin, Mats
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The FEBS Journal
- volume
- 275
- issue
- 22
- pages
- 5657 - 5666
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:18959751
- wos:000260348600016
- scopus:54849437088
- pmid:18959751
- ISSN
- 1742-464X
- DOI
- 10.1111/j.1742-4658.2008.06691.x
- project
- Human IgE repertoires and an anti-allergome resource
- language
- English
- LU publication?
- yes
- id
- 574e63fd-423f-47e6-8b25-3ecd33551914 (old id 1245883)
- date added to LUP
- 2016-04-01 13:12:02
- date last changed
- 2022-01-27 17:56:26
@article{574e63fd-423f-47e6-8b25-3ecd33551914,
abstract = {{Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders.}},
author = {{Persson, Jonas and Augustsson, Per and Laurell, Thomas and Ohlin, Mats}},
issn = {{1742-464X}},
language = {{eng}},
number = {{22}},
pages = {{5657--5666}},
publisher = {{Wiley-Blackwell}},
series = {{The FEBS Journal}},
title = {{Acoustic microfluidic chip technology to facilitate automation of phage display selection}},
url = {{http://dx.doi.org/10.1111/j.1742-4658.2008.06691.x}},
doi = {{10.1111/j.1742-4658.2008.06691.x}},
volume = {{275}},
year = {{2008}},
}