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Acoustic microfluidic chip technology to facilitate automation of phage display selection

Persson, Jonas LU ; Augustsson, Per LU ; Laurell, Thomas LU and Ohlin, Mats LU (2008) In The FEBS Journal 275(22). p.5657-5666
Abstract
Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the... (More)
Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The FEBS Journal
volume
275
issue
22
pages
5657 - 5666
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:18959751
  • wos:000260348600016
  • scopus:54849437088
ISSN
1742-464X
DOI
10.1111/j.1742-4658.2008.06691.x
project
CREATE Health
language
English
LU publication?
yes
id
574e63fd-423f-47e6-8b25-3ecd33551914 (old id 1245883)
date added to LUP
2008-10-14 10:23:10
date last changed
2017-09-03 04:03:10
@article{574e63fd-423f-47e6-8b25-3ecd33551914,
  abstract     = {Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders.},
  author       = {Persson, Jonas and Augustsson, Per and Laurell, Thomas and Ohlin, Mats},
  issn         = {1742-464X},
  language     = {eng},
  number       = {22},
  pages        = {5657--5666},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {The FEBS Journal},
  title        = {Acoustic microfluidic chip technology to facilitate automation of phage display selection},
  url          = {http://dx.doi.org/10.1111/j.1742-4658.2008.06691.x},
  volume       = {275},
  year         = {2008},
}