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The contribution of photosynthetic pigments to the development of biochemical separation methods: 1900-1980

Albertsson, Per-Åke LU (2003) In Photosynthesis Research 76(1-3). p.217-225
Abstract
The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracentrifuge in the 1920s. A. Tiselius improved electrophoresis in the 1930s and developed chromatography of proteins in the 1940s and 1950s. Others of `The Uppsala school in separation science' include J. Porath, P. Flodin and S. Hjertén who further developed various gel chromatographic methods. Hjertén introduced free zone electrophoresis in narrow tubes, a forerunner of capillary electrophoresis. Two proteins, phycoerythrin and... (More)
The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracentrifuge in the 1920s. A. Tiselius improved electrophoresis in the 1930s and developed chromatography of proteins in the 1940s and 1950s. Others of `The Uppsala school in separation science' include J. Porath, P. Flodin and S. Hjertén who further developed various gel chromatographic methods. Hjertén introduced free zone electrophoresis in narrow tubes, a forerunner of capillary electrophoresis. Two proteins, phycoerythrin and phycocyanin, were used as test substances in all these methodological studies. Aqueous two-phase partitioning as a separation method was introduced in 1956 by the author. In this work, chloroplast particles were used, and the method was applied for the separation and purification of intact chloroplasts, inside-out thylakoid vesicles and plasma membranes. My research was carried out in cooperation with G. Blomquist, G. Johansson, C. Larsson, B. Andersson and H.-E. Akerlund during a 20-year period, 1960-1980. (Less)
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published
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keywords
J. Porath, C. Larsson, S. Hjertén, P. Flodin, electrophoresis, T. Svedberg, chromatography, centrifugation, aqueous two-phase partition, B. Andersson, P-A. Albertsson, chloroplast, H-E. Akerlund, A. Tiselius, M. Tswett, ultracentrifuge
in
Photosynthesis Research
volume
76
issue
1-3
pages
217 - 225
publisher
Springer
external identifiers
  • wos:000184422000019
  • scopus:0042697620
ISSN
0166-8595
DOI
10.1023/A:1024944606930
language
English
LU publication?
yes
id
eab55347-e1cf-49aa-a6a9-6308d05f97c3 (old id 124594)
date added to LUP
2007-06-26 10:42:31
date last changed
2018-05-29 10:55:53
@article{eab55347-e1cf-49aa-a6a9-6308d05f97c3,
  abstract     = {The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracentrifuge in the 1920s. A. Tiselius improved electrophoresis in the 1930s and developed chromatography of proteins in the 1940s and 1950s. Others of `The Uppsala school in separation science' include J. Porath, P. Flodin and S. Hjertén who further developed various gel chromatographic methods. Hjertén introduced free zone electrophoresis in narrow tubes, a forerunner of capillary electrophoresis. Two proteins, phycoerythrin and phycocyanin, were used as test substances in all these methodological studies. Aqueous two-phase partitioning as a separation method was introduced in 1956 by the author. In this work, chloroplast particles were used, and the method was applied for the separation and purification of intact chloroplasts, inside-out thylakoid vesicles and plasma membranes. My research was carried out in cooperation with G. Blomquist, G. Johansson, C. Larsson, B. Andersson and H.-E. Akerlund during a 20-year period, 1960-1980.},
  author       = {Albertsson, Per-Åke},
  issn         = {0166-8595},
  keyword      = {J. Porath,C. Larsson,S. Hjertén,P. Flodin,electrophoresis,T. Svedberg,chromatography,centrifugation,aqueous two-phase partition,B. Andersson,P-A. Albertsson,chloroplast,H-E. Akerlund,A. Tiselius,M. Tswett,ultracentrifuge},
  language     = {eng},
  number       = {1-3},
  pages        = {217--225},
  publisher    = {Springer},
  series       = {Photosynthesis Research},
  title        = {The contribution of photosynthetic pigments to the development of biochemical separation methods: 1900-1980},
  url          = {http://dx.doi.org/10.1023/A:1024944606930},
  volume       = {76},
  year         = {2003},
}