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No long-term persistence of bacterial pollution-induced community tolerance in tylosin-polluted soil

Aldén, Louise LU and Bååth, Erland LU (2008) In Environmental Science & Technology 42(18). p.6917-6921
Abstract
Pollution-induced community tolerance (PICT) of soil bacteria to the antibiotic tylosin was studied over 95 days. Tylosin was added at increasing concentrations, together with different amounts of alfalfa to study the effects of substrate addition on PICT and bacterial growth in soil. The leucine incorporation technique was used to estimate bacterial growth and as a detection method in the PICT concept. Direct inhibition of the bacterial growth rates, resulting in a dose-response curve, was found above 50 mg of tylosin kg(-1) of soil two days after tylosin addition (IC50 value of 960 mg tylosin kg(-1)). After 10 days of exposure to at least 50 mg of tylosin kg(-1), the PICT was observed and correlated to inhibition of bacterial growth by... (More)
Pollution-induced community tolerance (PICT) of soil bacteria to the antibiotic tylosin was studied over 95 days. Tylosin was added at increasing concentrations, together with different amounts of alfalfa to study the effects of substrate addition on PICT and bacterial growth in soil. The leucine incorporation technique was used to estimate bacterial growth and as a detection method in the PICT concept. Direct inhibition of the bacterial growth rates, resulting in a dose-response curve, was found above 50 mg of tylosin kg(-1) of soil two days after tylosin addition (IC50 value of 960 mg tylosin kg(-1)). After 10 days of exposure to at least 50 mg of tylosin kg(-1), the PICT was observed and correlated to inhibition of bacterial growth by tylosin. A return of the PICT to control levels was found over time, and after 95 days at 1500 mg of tylosin kg(-1), essentially no PICT was found, as compared to the unpolluted control soil. The return of PICT to pre-exposure levels was not totally reflected in the recovery of bacterial growth. Alfalfa addition did not affect the inhibitory effect of tylosin on bacterial growth rates; neither did it alter the PICT. Since tylosin is relatively rapidly degraded in soil, our results indicate that the PICT will return to prepollution levels when the selective pressure of the toxicant is removed and will thus be a useful technique for monitoring remediation measures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Science & Technology
volume
42
issue
18
pages
6917 - 6921
publisher
The American Chemical Society
external identifiers
  • wos:000259139400026
  • scopus:52349101937
ISSN
1520-5851
DOI
10.1021/es8004706
language
English
LU publication?
yes
id
6227e40b-b381-4091-9b71-7d43f619c958 (old id 1246674)
date added to LUP
2008-11-18 14:00:48
date last changed
2017-06-04 03:55:19
@article{6227e40b-b381-4091-9b71-7d43f619c958,
  abstract     = {Pollution-induced community tolerance (PICT) of soil bacteria to the antibiotic tylosin was studied over 95 days. Tylosin was added at increasing concentrations, together with different amounts of alfalfa to study the effects of substrate addition on PICT and bacterial growth in soil. The leucine incorporation technique was used to estimate bacterial growth and as a detection method in the PICT concept. Direct inhibition of the bacterial growth rates, resulting in a dose-response curve, was found above 50 mg of tylosin kg(-1) of soil two days after tylosin addition (IC50 value of 960 mg tylosin kg(-1)). After 10 days of exposure to at least 50 mg of tylosin kg(-1), the PICT was observed and correlated to inhibition of bacterial growth by tylosin. A return of the PICT to control levels was found over time, and after 95 days at 1500 mg of tylosin kg(-1), essentially no PICT was found, as compared to the unpolluted control soil. The return of PICT to pre-exposure levels was not totally reflected in the recovery of bacterial growth. Alfalfa addition did not affect the inhibitory effect of tylosin on bacterial growth rates; neither did it alter the PICT. Since tylosin is relatively rapidly degraded in soil, our results indicate that the PICT will return to prepollution levels when the selective pressure of the toxicant is removed and will thus be a useful technique for monitoring remediation measures.},
  author       = {Aldén, Louise and Bååth, Erland},
  issn         = {1520-5851},
  language     = {eng},
  number       = {18},
  pages        = {6917--6921},
  publisher    = {The American Chemical Society},
  series       = {Environmental Science & Technology},
  title        = {No long-term persistence of bacterial pollution-induced community tolerance in tylosin-polluted soil},
  url          = {http://dx.doi.org/10.1021/es8004706},
  volume       = {42},
  year         = {2008},
}