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Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions

Lundqvist, Jon LU ; Jacobs, Anna; Palm, Magnus LU ; Zacchi, Guido LU ; Dahlman, Olof and Stålbrand, Henrik LU (2003) In Carbohydrate Polymers 51(2). p.203-211
Abstract
Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was... (More)
Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was obtained from water impregnated spruce chips heat-fractionated at 190oC for 5min (MW of 3800). The highest MW (14,000) was obtained from impregnation with 2% NaOH (190oC, 5min), but the yield of mannan was very low (3%). Impregnation with 0.025% NaOH and heat-fractionation at 190oC for 5min resulted in extraction of GGM with MW of 9500 and a mannan yield of 31%. When the spruce chips were impregnated with =<0.05% NaOH an O-acetyl-galactoglucomannan was extracted, whereas when higher NaOH charges were used in the impregnation, the extracted GGM lacked acetyl groups. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Spruce (Picea abies), Hemicellulose, O-acetyl-galactoglucomannan, Microwave oven, Size-exclusion chromatography, MALDI-MS
in
Carbohydrate Polymers
volume
51
issue
2
pages
203 - 211
publisher
Elsevier
external identifiers
  • wos:000178715500009
  • scopus:0037289902
ISSN
0144-8617
DOI
10.1016/S0144-8617(02)00111-X
language
English
LU publication?
yes
id
bd52b908-c2d1-4e20-9b5e-688e2595acfa (old id 124689)
date added to LUP
2007-07-06 10:14:14
date last changed
2018-10-03 11:25:57
@article{bd52b908-c2d1-4e20-9b5e-688e2595acfa,
  abstract     = {Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was obtained from water impregnated spruce chips heat-fractionated at 190oC for 5min (MW of 3800). The highest MW (14,000) was obtained from impregnation with 2% NaOH (190oC, 5min), but the yield of mannan was very low (3%). Impregnation with 0.025% NaOH and heat-fractionation at 190oC for 5min resulted in extraction of GGM with MW of 9500 and a mannan yield of 31%. When the spruce chips were impregnated with =&lt;0.05% NaOH an O-acetyl-galactoglucomannan was extracted, whereas when higher NaOH charges were used in the impregnation, the extracted GGM lacked acetyl groups.},
  author       = {Lundqvist, Jon and Jacobs, Anna and Palm, Magnus and Zacchi, Guido and Dahlman, Olof and Stålbrand, Henrik},
  issn         = {0144-8617},
  keyword      = {Spruce (Picea abies),Hemicellulose,O-acetyl-galactoglucomannan,Microwave oven,Size-exclusion chromatography,MALDI-MS},
  language     = {eng},
  number       = {2},
  pages        = {203--211},
  publisher    = {Elsevier},
  series       = {Carbohydrate Polymers},
  title        = {Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions},
  url          = {http://dx.doi.org/10.1016/S0144-8617(02)00111-X},
  volume       = {51},
  year         = {2003},
}