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A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2

Burbelo, Peter D. ; Hirai, Hiroki ; Leahy, Hannah ; Lernmark, Ake LU orcid ; Ivarsson, Sten LU ; Iadarola, Michael J. and Notkins, Abner Louis (2008) In Diabetes Care 31(9). p.1824-1826
Abstract
OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison... (More)
OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes Care
volume
31
issue
9
pages
1824 - 1826
publisher
American Diabetes Association
external identifiers
  • wos:000258868800022
  • scopus:56149084386
  • pmid:18535195
ISSN
1935-5548
DOI
10.2337/dc08-0286
language
English
LU publication?
yes
id
72626f22-9a99-47f9-b7f8-52106152e979 (old id 1247841)
date added to LUP
2016-04-01 13:47:42
date last changed
2025-04-04 14:25:55
@article{72626f22-9a99-47f9-b7f8-52106152e979,
  abstract     = {{OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.}},
  author       = {{Burbelo, Peter D. and Hirai, Hiroki and Leahy, Hannah and Lernmark, Ake and Ivarsson, Sten and Iadarola, Michael J. and Notkins, Abner Louis}},
  issn         = {{1935-5548}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1824--1826}},
  publisher    = {{American Diabetes Association}},
  series       = {{Diabetes Care}},
  title        = {{A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2}},
  url          = {{http://dx.doi.org/10.2337/dc08-0286}},
  doi          = {{10.2337/dc08-0286}},
  volume       = {{31}},
  year         = {{2008}},
}